Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... soybean b-amylase (Cys82, Cys97, Cys208 and Cys343).
On the analogy of the soybean b-amylase, the active
site of the C. sepium b-amylase most probably consists
of a cleft located between the barrel ... method.
Inhibition of the enzyme activity by glucose, maltose and
cyclohexaamylose
For the study of the enzyme inhibition by glucose, maltose
and cyclohex...
... apical membranes of the
oxyntic-peptic cells [37]. The distribution of the ecto-
ATPDase on these epithelial cells is distinctly different from
theotherATPDaseintheE-ATPasefamily,theCD39s
[13,17,19].
Molecular ... ATPase
activity at either pH 7.4 or pH 6.4 (Fig. 3). At pH 7.4,
5m
M
azide inhibited ADP hydrolysis by 70% whereas
ATP hydrolysis was inhibited by only 10%. Inhibition...
... of the 47th Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 746–754,
Suntec, Singapore, 2-7 August 2009.
c
2009 ACL and AFNLP
What lies beneath: Semantic and syntactic analysis
of ... argument, the most common of
which were CAU and LOC.
Reconstructive substitutions
Q: How often do substitutions occur in the an-
alyzed data, and is there any...
... currently the pitch con-
tour and pauses, are extracted by hardware and
software. The hardware detects pitch and paus-
es from the speech waveform, while the software
determines the duration of ... words, morphemes and pauses) and
rhythm. While all of these are important cues, we
are currently focussing on pitch and pauses as
these are easily extracted from...
... hindrance to
the side chain of Lys9 and Hyp10 in forming
the tight loop as in conomarphin. The side
chain of Lys9, Hyp10 and
D-Phe13 of cono-
marphin is shown in stick mode, and the
side chain of
L-Phe13 ... in
supplementary Fig. S2A and P3 in supplementary
Fig. S2B).
To narrow the range of the possible position of the
d-amino acid, chymotrypsin was used to d...
... Legrain C, Boyen A & Glansdorff N (1998)
Genes and enzymes of the acetyl cycle of arginine
biosynthesis in the extreme thermophilic bacterium
Thermus thermophilus HB27. Microbiology 144,
479–492.
K. ... N-acetyltransferase
therefore recycles the acetyl moiety of N-acetyl-
ornithine, regenerating N-acetylglutamate in citrulline
and arginine biosynthesis. This enzyme is func...
... middle and C-terminal
region of the HdEG66, respectively, from the sequence
similarity to the termite cellulase. Then, a forward primer F1
was synthesized on the basis of the N-terminal sequence of
the ... ACNATHWSNAAYCAYTGGGA
LP1 DAYATTK
LP2 WRGDSALGDK
LP3 GDNGEDLTGGWY
LP4 TEVEGFFK
LP5 YPGIYSSSIQDAGQFYSSSGYK R1 RTARAAYTGNCCNGCRTCYTG
LP6 WAVEQMNYILGDNK R2 CATYTGYTCNACNGC...
... jacquemontii
lectin
The Paratelphusa lectin agglutinated only a limited range of
erythrocytes. Out of 12 erythrocyte types tested the lectin
could agglutinate only six erythrocyte types (Table 2). Our
study on the ... 9-O-acetyl and 8,9di-O-acetyl
forms [41] on de-O-acetylation lost its inhibitory potency
completely, thus suggesting the importance of O-acetyl
NeuAc in the bi...
... APDyTTPEMY-NH
2
were obtained from Dr A
˚
ke
Engstro
¨
m at the Peptide Synthesis and Analysis Laboratory
of the Department of Medical Biochemistry and Microbio-
logy, Uppsala University. The ... obtained from Promega, and LysC of
Achromobacter lytii from Wako. The O-phosphorylated
phosphopeptides EQTRsLDGR-NH
2
, DHTGFLtEY-
VATRWC-NH
2
, DADEyL-NH
2
, EGDNDyIIPL-N...
... of the A201A biosyn-
thetic pathway, may be related to their counterparts of the
puromycin and hygromycin A biosynthetic pathways,
respectively.
The puromycin biosynthetic gene cluster (pur )from
S. ... permitted the isola-
tion of Streptomyces antibioticus genes implicated in
oleandomycin biosynthesis [40].
Gene analyses and enzymatic assays suggest that the
biosynthetic...