Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf
Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf
... Both transitions to latent and substrate forms were strongly but differently influenced by the amino-acid composition of the shutter region. Surprisingly, we foundthat substitution of Lys335 to ... to as the shutter region [2]. By high resolution X-raycrystal structure analysis of the native form of the serpin plasminogen activator inhibitor-2 (PAI-2) and the P1–P10cleaved form of horse ... of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms Martin Hansen, Marta N. Busse and Peter A. AndreasenLaboratory of Cellular Protein Science, Department of Molecular and...
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Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf
... becausemethylation by Dnmt3a is the initial event for de novomethylation and due to its own de novo activity Dnmt1might be able to stimulate itself in an autocatalytic fashion,iftoomuchofthisenzymeisused.We ... development and evenin adult cells to silence acquired proviral DNA or to alter the developmental program of the cell. Aberrant de novomethylation may lead to hypermethylation of promotorregions of ... fast. Therefore, this effectcan only double the rate of DNA methylation, becauseevery turnover by Dnmt3a can trigger only one turnover byDnmt1 in the opposite strand of the DNA. Consequently,this...
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Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field pptx
... previously [20]. The serine, threonine and tyrosinecontents of the hydrolysates were corrected for destructionduring the hydrolysis by extrapolation to zero time hydro-lysis. The amino acid compositions, ... very similar to the WSM, and consequently the EDTA-IM. Hydroxyproline, hydroxy-lysine and phosphoserine were not detected in WSM, WIM,EDTA-SM and EDTA-IM. The comparison of the amino acid composition ... decalcificationstep. Another reason, related to the first one, may be the accessibility of the acidic molecules. Being easily solubilized,it was possible to use these molecules to perform tests in vitro for their...
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Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations influence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx
... decades of life and severely impair visualacuity in adulthood. Their therapy is restricted to keratopl-asty and phototherapeutic keratectomy by Excimer laser.Unfortunately, the benefits of these therapies ... degree of amyloid fibril formation.Cysteine has the capacity to form disulfide bonds. Asdisulfide bonding of the protein may participate in the appearance of aggregates and predispose to the formation of ... that Cys–Cys bondformation does facilitate amyloid precipitation, but is not the only factor responsible for the amyloidogenicity of C110)131 peptide. The participation of other factors inamyloidogenesis,...
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Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx
... liquidsamplesweretakenandanalysedbyGCandGC-MStodetermine the products formed.ElectrophoresisSDS/PAGE was used to assess purity of enzyme prepara-tions and determine the molecular mass of the purifiedenzyme ... as the standard.RESULTSInduction of citral lyaseInitially, the reproducibility of the results was hindered byvariations in the citral lyase activity of the P. digitatumspores. Remarkably, ... citral lyase also convertedmethyl-crotonaldehyde and decanal forming acetone and octanal, respectively. Furthermore, cinnemaldehyde wasconverted into benzaldehyde, one of the most frequentlyapplied...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... The phosphoryl and pyridoxyl moieties of the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solventexposed area of the P-pyridoxyl group is 0.39 and 0.082 for the ... 2002conformational changes caused by the ligand are easilysensed by the P-pyridoxyl chromophore. Figure 4 shows the modeled structure of the P-pyridoxyl-PEP carboxykinase in the free and substrate ... another substrate of the enzyme, also increased the P-pyridoxylfluorescence. The addition of this substrate to the proteinblue shifted the maximum by 5 nm and the fluorescenceFig. 3. Steady state...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... wererecently introduced for the preparation of C-6 aldehydederivatives of Glc [15]. The key step is the use of Collinsreagent for the selective oxidation of the primary trimethyl-silyl ether of the ... G. (1986) The oxidation of primary trimethylsilyl ethers to aldehydes: a selective conversion of a primary hydroxy group intoan aldehyde group in the presence of a secondary hydroxy group.J. ... inactivation. To the contrary, the presence of Glc slightly sensitized EIIGlc for inactivation by the isothiocyanates 1e and 3d. On the other hand, the rate of inactivation by bromoacetyl-Glc (1a)...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... 2 h and 20 h to determine the substrate specificity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed byHPAEC and MALDI-TOF MS. HPAEC analysis ... producingtransformant and was used for the purification of GALA.Growth of this transformant and subsequent purification of the enzyme were performed as described in Materials and methods. Purity of the ... mMsodiumhydroxide for 40 minD-galactose,D-galactobiose and D-galactotetraose were used as standards to identify the D-galactose and D-galacto-oligosaccharides. The calculatedareas for D-galactose and D-galacto-oligosaccharides...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... above.Assay for superoxide dismutase activity The specific activity of SOD was measured spectrophoto-metrically by its inhibitory action on the superoxide-dependent reduction of cytochrome c by xanthine-xanthineoxidase ... conclusiveresult.Effect of hydrogen peroxide on enzyme activityHydrogen peroxide, the product of the SOD reaction, isan inhibitor of SODs and an inactivator of FeSOD. It isoften used to distinguish between the ... represents either iron or manganese.Selectivity of the proteins for their metal cofactor has beendemonstrated in vivo [3] and although apoenzymes of eachtype of SOD may be reconstituted by the addition...
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Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
... wasrestricted to the )60° conformer for the DLform of residueDandto+60° for the LDform. For the trisaccharide, 5 · 103macro moves were used with a step length of 10° for the glycosidic linkage and ... Subsequent1D-NOESY-TOCSY (H-8D, 200 ms; H-6D,60 ms) for detection of the H-7D and H-7¢Dresonances. (d) 1D-TOCSY-NOESY (H-4DH-8D, 30 ms; H-5D, 200 ms) for the detection of NOEs for H-5D. (e) 1D-TOCSY-NOESY(H-5D, ... thispossibility. Alternatively, this enzyme may be involved in the epimerization reaction necessary for the biosynthesis of the capsular heptopyranose as alditol acetate analysis(results not shown) and...
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