... °Cfor1h.Thus treated proteins w ere extracted with SDS and then analyzed bySDS/PAGE, followed by CBB staining and autoradiography. Lanes 1 and 4 , GST; lanes 2 and 5, GST–NK; l anes 3 and 6, ... Primary structure analysis, and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane. J. Biol. Chem. 26 9, 11 306– 11 311 . 16 . Silve, S., Dupuy, P H., Ferrara, ... the activation of tryptophan 5-mono-oxygenase. Eur. J. Bioc hem. 1 32, 15 21 . 26 . Smythe, C. & Newport, J.W. (19 91) Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown...