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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx

Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx

Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx

... Biochem. 269) Ó FEBS 2002 Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon -c through the upregulation of protein kinase C in rat ... Japan The effect of a-tocopheryl hemisuccinate (TS) on lipo-polysaccharide (LPS) /interferon -c (IFN) -induced nitric oxide production in rat vascular smooth muscle cells (VSMC) was examined. The ... a-tocopherol; TA, a-tocopheryl acetate; TN, a-tocopheryl nicotinate; TRAF, tumor necrosis factorreceptor; TS, a-tocopheryl hemisuccinate; VSMC, vascular smooth muscle cells. Enzymes: nitric oxide...
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Tài liệu Báo cáo khoa học: Mixed-type noncompetitive inhibition of anthrax lethal factor protease by aminoglycosides ppt

Tài liệu Báo cáo khoa học: Mixed-type noncompetitive inhibition of anthrax lethal factor protease by aminoglycosides ppt

... the specificity in inhibitorbinding using a similar method. The purpose of this study was twofold. First, wewished to elucidate the kinetic mechanism of inhibition by which neomycin B and other ... nonlinear fit of the reaction pro-gress curves, rather than relying on routinely used lin-ear fit of an arbitrarily chosen initial portion of eachkinetic trace. Applying linear regression of the ... screened a diverse library of natural and synthetic compounds in vitro and discovered thatpolycationic aminoglycosides, such as neomycin B, arevery potent inhibitors. In a follow-up study in...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx

Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx

... sequences: PhiC: 5¢-CGATACGCTCAAAGTCAAAATAATCAGCGTGACATTCAGAAGGGTAATAAGAACG-3¢;, PhiW: 5¢-CGTTCTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCG-3¢ and M1 3C: 5¢-CTACAACGCCTGTAGCATTCCACAGACAGCCCTCATAGTTAGCGTAACGAGATCG-3¢. ... preponderance of octameric rings [13]. Recentstudies showed that Ca2+enhances the strandexchange activity of human Dmc1 protein by increas-ing the affinity of Dmc1 protein to ATP, mediated by a conformational ... emission increase(indicative of lack of strand exchange) was detected in the absence of OsDmc1 protein (Fig. 4A, line 1). Inter-estingly, the rate as well as the steady state levels of strand exchange...
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Báo cáo Y học: Permeability transition-independent release of mitochondrial cytochrome c induced by valinomycin ppt

Báo cáo Y học: Permeability transition-independent release of mitochondrial cytochrome c induced by valinomycin ppt

... noteworthy that the amount of cytochrome c Fig. 4. Effects of CsA and BKA on the release of cytochrome c frommitochondria induced by Ca2+ and by valinomycin. During the meas-urement of respiration, ... CsA and BKA wereineffective in preventing accelerated respiration (Fig. 1B). In contrast, as shown in Fig. 1C, valinomycin accelerated the rate of oxygen consumption, and the acceleratedrespiration ... for each condition, typical images are shown.Ó FEBS 2002 Cytochrome c release induced by valinomycin (Eur. J. Biochem. 269) 5227released by valinomycin was larger than that induced by Ca2+....
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

... Molecular model of the P-pyridoxyl-E. coli PEP carboxykinaseadduct. Nefrom Lys288 of the open structure of E. coli PEP carb-oxykinase (1OEN) is covalently linked to the carbonyl carbon of the P-pyridoxyl ... afunction of protein size and dynamics. Quenching experi-ments by acrylamide in the presence of several combina-tions of substrates and divalent ions at saturatingconcentrations were carried out by ... modeledusing the crystalline coordinates of the free E. coli PEPcarboxykinase [2]. The deviation of the resulting modelstructure (P-pyridoxyl labeled protein) from the coordinates of the starting...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

... d(5¢-AACAACGCAGCTGGGCTCTGGAACCAT), ECF-A141Q d(5¢-TCAACCTCTAACCAGGCTACTCCGCTG) ECM-G77Q d(5¢-AACAACGCTGG C CAGCACGCTAACCAC) and ECM-Q146A d(5¢-TCTACTGCTAACGCGGATTCTCCGCTG) following the manufacturer’s ... the primers ECM-5¢ d(5¢-AGCTATACCCTGCCATCCCTG) and ECM-3¢ d(5¢-TTATTTTTTCGCCGCAAAACGTG) and E. coli genomic DNA as template. PCR was carried outusing Amplitaq enzyme according to the manufacturer’sinstructions ... 1 and 2). The differen-tial effects of hydrogen peroxide on the FeSOD mutantsmay be explained by an alteration of reactivity induced by the electronic configuration of active site residues, in...
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Tài liệu Báo cáo Y học: Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase ppt

Tài liệu Báo cáo Y học: Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase ppt

... stimulated the synthesis of poly(A)catalyzed by yeast poly(A) polymerase. The relativeactivity of diadenosine polyphosphates as effectors of the poly(A) synthesis was assayed as in Fig. 4, using0.05 ... could opennew views both on the catalytic properties of yeast poly(A)polymerase and on the intracellular role of dinucleosidepolyphosphates, a family of compounds of increasingmetabolic and ... novelproperty of E.colipoly(A) polymerase moved us to analyzewhether these compounds were also substrates of eukaryoticyeast poly(A) polymerase. The yeast enzyme is involved in the processing of the...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt

Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt

... overNi-nitrilotriacetic acid resin, according to the instructions of the manufacturer. A single protein band was detected by SDS/PAGE of purified enzymes.Enzyme activities were determined by measuring the formation ... 5¢-ATTAATGGCATGCTTTACCCGT-3¢.UsingthePCRproducts of agmatinase with the GfiAandCfiT substi-tutions in the coding and noncoding strands, respectively, and using the and 3¢ primers mentioned above, the ... de Concepcio´n, Casilla 160 -C, Concepcio´n,Chile;2Center for Genetic Engineering and Biotechnology, Habana, CubaUpon mutation of Asp153 by asparagine, the catalyticactivity of agmatinase...
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Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc

Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc

... bis-hydroxymethylated. The purity of these compounds (> 96%) was checked by 1HNMR and HPLC.Spectrophotometric determination of laccase activity The activity of laccase was determined by following the rate of ... evidence, and in contrast with the outcome of the laccase and laccase/HPI reactions, the only productobserved (by 1H-NMR), when substrate 4 is reacted with the laccase/TEMPO system in the mixed ... our laccase oxidations of substrates 1–5, and the effect of the cosolvent and of the mediators on the amount of substrate metabolized by the enzyme.(a) Laccase and laccase/HPI. In general, the...
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Tài liệu Báo cáo Y học: DNA and RNA damage by Cu(II)-amikacin complex docx

Tài liệu Báo cáo Y học: DNA and RNA damage by Cu(II)-amikacin complex docx

... caused by the insertion of mistranslated proteins into the cytoplasmic membrane of Escherichia coli and subsequent caging of the antibiotic inside the cells due to degradation of these proteins. ... Poland;3Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland The oxidation-promoting reactivity of copper(II) complex of aminoglycosidic antibiotic amikacin [Cu(II)-Ami] ... generation of hydroxylradicals by another aminoglycoside, gentamicin has recentlybeen implicated in the mechanism of its toxicity [18,19].Aminoglycosides themselves, including Ami, are redox-inactive...
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