... the Uterals
The final step in assembling the proof net is to con-
nect together the literal nodes at the top of the
graph. It is at this stage that unification is applied
to the variables ... survive the hypothetical and so cannot affect the
meaning of the licenser in some other way. That is,
the licensing constructor (£ o (A ® l)) o B can
derive all of the...
... TTGAGGTGACAGACAATTGCCT
RPE65c GSP-Rev TCTTTGACTTCTCAAACTGATCG
RPE65a-His-Fwd
NM_200751 GCGGCCGCCACCATGCATCATCACCATCAC
CATGTCAGCCGTTTTGAACAC
RPE65c-His-Fwd
NM_001113653 GCGGCCGCCACCATGCATCATCACCATCAC
CATGTCAGCCGTCTTGAACAC
Y. ... retinol
(atROL) as the substrate for the conversion of
11cROL [24–27]; the substrate of RPE65 in the RPE is
atRE [12]. To verify the substrate specificity of...
... represent the flexible
residues within the protein domain. NMR data
provide evidence that the interaction site on the RNA
is the phosphate backbone. This is also in accordance
with the demonstrated ... assay, even at very low salt
concentrations. As the latter assay would require the
formation of a stable complex, the formation of only
transiently populated RNAÆ protein com...
... MAPK-mediated phosphor-
ylation of Bax at Thr167, leading to its activation in
HepG2 cells [122]. On the other hand, treatment with
OA increases Akt-mediated phosphorylation of Bax at
Ser184, ... intri-
cately interrelated; it was previously shown that either induction or inhibi-
tion of phosphorylation causes cell death. Determination of the
relationship between protein and phosphory...
... hypothesize that the proton-
ated state of the chaperone initiates this cycle, whereas
the deprotonated state occurs upon completion of the
maturation process of the partner. The nature of the
signal ... from the heat of the reaction to
obtain the effective heat of binding [27].
DSC
Heat denaturation measurements were carried out on a
MicroCal VP-DSC instrument (Microcal L...
... within the GBD part of the
protein. Another possible explanation for the observed
autoinhibitory activity is that the Hth domain mediates
some intramolecular interaction, or affects the confor-
mation ... indicates the base that correlates with alternative or constitutive splicing. (B) The presence of alternative splicing
around the 5¢-end of exon 6 of Meis2 and Meis3 was test...
... that the
cathepsin B mutations do not affect the geometry of
binding of chagasin). The wild-type sequences have
also been preserved in the related structural studies of
procathepsin B [22].
These ... [19]. The mini loop in carboxypeptidase
cathepsin X blocks the primed side of the active site,
restricting access to only one residue [20].
Although the structures of the mature...
... model
dipalmitoylphosphatidylcholine bilayer in the presence of the 40-residue Ab
peptide (Ab40). The simulated systems examine the effects of the insertion
depth of the peptide, temperature, the protonation state ... salt concentration. Simulation set B
examined the effects of both the protonation state of
the peptide and temperature on the behavior of the
system. Fin...
... investigated the effects of the mutation on
the enzymatic maturation of FVIIa and on the
response of FVIIa to TF.
Our measurements, in both the presence and absence
of TF, revealed that the amidolytic ... effects of the G372(223)A mutation is slightly
substrate-dependent. The fact that the relative reduc-
tion in activity was not greater in the presence of
cofactor strongl...
... that they comple-
ment each other.
Other classification techniques
Other classification techniques were investigated to
evaluate whether they could add improvements to the
new method. To further ... that look only at stability changes of the mutation
compared with the wild-type protein [22,31].
Matthews’ correlation coefficient
Matthews’ correlation coefficient (MCC) [37] was used to
esti...