... suppliedwith the enzyme, and contained Aba, Abb and Abcat40 nm each, and the start and stop primers Abstart and Abstop at 600 nm each, and 200 lm each of dATP, dCTP,dGTP and dTTP. The product ... USA), and purified using reverse-phase HPLC. For both synthetic and recombinant Ab peptides, thecorrect mass was confirmed by MALDI-TOF MS and LC-MS.PCR and cloning procedureSynthetic genes for ... acarbon-coated formvar grid, left for 1 min, fixed with glu-taraldehye, wicked dry with filter paper, and 2% uranylacetate was added and the mixture was incubated for 2 min. The grid was wicked dry and...