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Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx

Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx

Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx

... Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin Tianbao Chen1,2, David F. Orr1, Anthony J. Bjourson1, Stephen ... Previously reported bradykinins from other Bombina species were not detected. Respective precursor cDNAs, designated BVK-1 and BVK-2, respect-ively, were cloned from a skin library by 3¢ -and5 ¢-RACEreactions. ... This sensitivity is well within the capabilityof the ES-MS system employed.The primary structural diversity displayed withinamphibian skin bradykinins may not simply be due tophylogenetic differences...
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Tài liệu Báo cáo Y học: Novel complexes of mammalian translation elongation factor eEF1AÆGDP with uncharged tRNA and aminoacyl-tRNA synthetase potx

Tài liệu Báo cáo Y học: Novel complexes of mammalian translation elongation factor eEF1AÆGDP with uncharged tRNA and aminoacyl-tRNA synthetase potx

... spectroscopy in peptide and protein analysis. In Encyclopedia of Analytical Chemistry: Instru-mentation and Applications (Meyers, R.A., ed.), pp. 5762–5779.John Wiley and Sons, New York.23. ... eEF1Badeparts from eEF1A being ousted by newly synthesizedaminoacyl-tRNA (E) [32]. The finding that the complex ofeEF1A, eEF1Ba and nonhydrolyzable analog of GTPcould be dissociated by aminoacyl-tRNA ... supported in part by theWellcome Trust Research Travel Grant and FEBS Short-termFellowship.REFERENCES1. Mirande, M. (1991) Aminoacyl-tRNA synthetase family from prokaryotes and eukaryotes: structural...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

... phosphoryl and pyridoxyl moietiesof the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solventexposed area of the P-pyridoxyl group is 0.39 and 0.082 for the open and ... 2002conformational changes caused by the ligand are easilysensed by the P-pyridoxyl chromophore. Figure 4 shows themodeled structure of the P-pyridoxyl-PEP carboxykinase inthe free and substrate bound conformations. ... the P-pyridoxyl-E. coli PEP carboxykinaseadduct. Ne from Lys288 of the open structure of E. coli PEP carb-oxykinase (1OEN) is covalently linked to the carbonyl carbon of theP-pyridoxyl group...
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

... obtained from Promega, and LysC ofAchromobacter lytii from Wako. The O-phosphorylatedphosphopeptides EQTRsLDGR-NH2, DHTGFLtEY-VATRWC-NH2, DADEyL-NH2, EGDNDyIIPL-NH2,KKPRyEIRW-NH2, and ... GRRPsLFG and APDyTTPEMY-NH2were obtained from Dr A˚keEngstro¨m at the Peptide Synthesis and Analysis Laboratoryof the Department of Medical Biochemistry and Microbio-logy, Uppsala University. The ... pre-ferentially in heart and skeletal muscle. These resultsprovide a new tool for studying eukaryotic histidinephosphorylation/dephosphorylation.Keywords: dephosphorylation; N-phosphorylation;...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

... exists in these two glycosylatedforms, but also as one most likely unglycosylated species(lane 3, band C), with mobility exactly as recombinantcystatin F after deglycosylation with PNGase F ... inhibitor, cystatin C. Cystatin F purified from lysates of U937 cells showed three major forms car-rying two, one or no carbohydrate chains. Immunocyto-chemistry demonstrated a marked cytoplasmic cystatin ... additional band (lane 3,band a)withMrapproximately 3200 Da higher than thediglycosylated cystatin F species. The nature of this band ispresently unknown and is discussed below.In order to study the...
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Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx

Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx

... identity). Shinmyo et al. [64] have studied the promoteractivity and wound-induction of HRPC and -E types, and of AtPCa and AtPEa. Most remarkably, C2, and only C2,responded strongly to wounding. ... flowers and siliques to represent whole healthy plantsat all ages [12]. Whole plants were analyzed at day 3, 6, 15, 35 and 59 after sowing. Roots were analyzed at day 15, 35 and 59, rosettes at day ... At4g37530 from acDNA library of Erysiphe cichoracearum infected plants(kindly provided by S. Somerville and I. Wilson, StandfordUniversity, USA). The gene for AtP37 follows the consti-tutively expressed...
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot

Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot

... and activation by onlytwo peptides from type II collagen (data not shown) and only by peptide a1(I) CB3 from t ype I collagen, as alreadyknown from the literature [49].Peptide CB10 from type ... thecharge.MATERIALS AND METHODSMaterialsType I collagen from bovine skin and its CNBr peptideswere already available and characterized by our laboratory[27–30].Sulfosuccinimidyl acetate, p-nitrophenyl phosphate,avidin ... iscrepancy could lie in the fact thattype II collagen is more glycosylated than type I collagen. Itseems to us probable that glycosylation of hydroxylysinewill block the binding. However, the glycosylation...
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Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf

Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf

... Henkler2,Ottmar Janssen3 and Harald Wajant11Institute of Cell Biology and Immunology and 2Institute of Industrial Genetics, University of Stuttgart, Germany;3Institute ofImmunology, Christian-Albrechts-University ... exons 7–10.Purification and stimulation of primary humanT-lymphocytesMononuclear cells (PBMNC) were isolated from periph-eral blood of healthy donors by Ficoll density centrifuga-tion. The ... by gray boxes. (B) Theindicated cells were fractionated and lysates containing cytoplasmic(C) and nuclear proteins (N) were analyzed by Western blotting.TRAF4 was detected using an affinity-purified...
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Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx

Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx

... phosphatidylinositol; PtdOH, phosphatidicacid; CDP-DAG, CDP-diacylglycerol; CMP, cytidinemonophosphate; CTP, cytidine triphosphate.Enzymes: phosphatidylinositol synthase or CDP-diacylglycerol/myo-inositol ... Phosphatidylinositol synthesis and exchange of the inositol headare catalysed by the single phosphatidylinositol synthase 1 from ArabidopsisAnne-Marie Justin, Jean-Claude Kader and Sylvie CollinUniversite´Pierre ... CMP-dependent PtdIns hydrolysis. (B)De novo synthesis (light grey), exchange activities (–CMP, striped bar;+CMP, medium grey) and CMP-dependent PtdIns hydrolysis (darkgrey) normalized to their respective...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

... DeBruyne, C.K. (1988) D-xylose isomerase from Streptomycesviolaceoniger: structural and catalytic roles of bivalent metal ions.Enzyme Microb. Technol. 10, 695–700.41. van Bastelaere, P., Vangrysperre, ... finally dialyzedtwice against 50 mM Mops (pH 7.0), this time withoutEDTA. The apoenzyme was divided into aliquots and storedat 2 70 8C until use.Enzyme assaysBLXI activity was assayed routinely ... 0.03 mM (activity onxylose). Activity on xylose was determined using0.024 mg·mL21apo-BLXI and 635 mM xylose. Activityon glucose was determined using 0.06 mg·mL21apo-BLXI and 1M glucose....
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