Tài liệu Báo cáo Y học: Study of substrate specificity of human aromatase by site directed mutagenesis pdf
Tài liệu Báo cáo khoa học: Molecular and cellular specificity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx
... PeptideSynthesizer (Applied Biosystems, Foster City, CA, USA) asdescribed previously [51]. The homogeneity of the syntheticpeptides preparation was assessed by MALDI-TOF MSand analytical RP-HPLC.Decapeptides ... 481–485.10 Ollivaux C & Soyez D (2000) Dynamics of biosynthe-sis and release of crustacean hyperglycemic hormoneisoforms in the X-organ-sinus gland complex of thecrayfish Orconectes limosus. ... (2008) Substrate specificity of platypus venom L-to-D-peptideisomerase. J Biol Chem (2008) 283, 8969–8975.17 Soyez D (2003) Recent data on the crustacean hypergly-cemic hormone family. In Recent...
- 13
- 687
- 0
Tài liệu Báo cáo Y học: Study of substrate specificity of human aromatase by site directed mutagenesis pdf
... relationships of human aromatase (Eur. J. Biochem. 269) 1405 Study of substrate specificity of human aromatase by site directed mutagenesis P. Auvray1,*,†, C. Nativelle1,†, R. Bureau2, P. Dallemagne2, ... mechanism of aromatase cytochromeP450. A site- directed mutagenesis study. Eur. J. Biochem. 268,243–251.63. Gaillard, J.L. (1991) Equine test icular aromatase: substrates specificity and kinetic ... thiscase, by introducing a hydroxyl group, accessibility of the substrate to the active site is reduced, as observed with themutants K119T and K11 9Y. By keeping an ionic residue,the binding affinity...
- 13
- 506
- 0
Tài liệu Báo cáo Y học: The RuvABC resolvasome Quantitative analysis of RuvA and RuvC assembly on junction DNA doc
... Rafferty3, Robert G. Lloyd2,Jane A. Grasby4and David P. Hornby11Transgenomic Research Laboratory, Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield,UK;2Institute ... overonly a 10-fold increase in protein concentration, nearly100% of the binding sites are occupied by RuvA. Theconcentration at which approximately 50% of the DNAbinding sites are bound by RuvA ... involves twokey steps: branch migration, catalysed by the RuvB proteinthat is targeted to the Holliday junction by the structurespecific RuvA protein, and resolution, which is catalysed by the RuvC...
- 10
- 672
- 0
Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... the cystatin F produced by U937 cells isretained intracellularly or is taken up by the cells immedi-ately following secretion. Accordingly, the relatively lowamount of cystatin F produced by ... to detect cystatin F directlyin culture media or cell lysates by immunoblotting. How-ever, by absorption on Sepharose-bound Cm-papain fol-lowed by Western blot analysis, the cell lysate-derivedinhibitor ... inhibitor, cystatin C. Cystatin F purifiedfrom lysates of U937 cells showed three major forms car-rying two, one or no carbohydrate chains. Immunocyto-chemistry demonstrated a marked cytoplasmic cystatin...
- 10
- 536
- 0
Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... DISCUSSION By site- directed mutagenesis, a D153N mutant form of E. coli agmatinase was obtained. The mutant enzymeretained about 5% of wild-type activity and it was equallyactive when assayed in ... (Asp153), may be reason-ably expected. This expectation is supported by our presentfindings of a markedly decreased activity of a D153Nvariant of E. coli agmatinase. From the enzymic properties of D153N ... D153N variant.Keywords: agmatinase; Asp153; site- directed mutagenesis; homology-modelling; E. coli.Agmatinase (agmatine ureohydrolase, EC 3.5.3.11) cata-lyses the hydrolysis of agmatine to putrescine...
- 5
- 475
- 0
Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf
... Wajant11Institute of Cell Biology and Immunology and2Institute of Industrial Genetics, University of Stuttgart, Germany;3Institute of Immunology, Christian-Albrechts-University of Kiel, GermanyTo gain ... 8).Correspondingly, fluorescence of cytoplasmic T4(259–470)–YFP and full-length T4–GFP was already significantly reduced after 2 min of bleaching in a small area of thecytoplasm, whereas the fluorescence of ... indicated by gray boxes. (B) Theindicated cells were fractionated and lysates containing cytoplasmic(C) and nuclear proteins (N) were analyzed by Western blotting.TRAF4 was detected using an affinity-purified...
- 11
- 468
- 0
Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx
... a cyclic diphosphate by theconsecutive action of 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, 4-diphosphocytidyl-2C-methyl-D-erythritolkinase and 2C-methyl-D-erythritol 2,4-cyclodiphosphatesynthase ... that[1-3H]2C-methyl-D-erythritol 4-phosphate was diverted effici-ently to the 2C-methyl-D-erythritol 2,4-cyclodiphosphateFig. 3.13C NMR signals of 2C-methyl-D-erythritol 2,4-cyclodiphosphate obtained by ... partialscrambling of label with the known lack of fidelity of the isopentenyl diphosphate/dimethylethyl diphosphateisomerase.Keywords: nonmevalonate pathway; terpene; chromoplasts;2C-methyl-D-erythritol...
- 9
- 572
- 0
Tài liệu Báo cáo Y học: The solution structure and activation of visual arrestin studied by small-angle X-ray scattering pot
... phosphorylation of the C-terminus of R* and binding by arrestin. Phosphorylation somewhatdecreases the ability of R* to signal transducin. Rapid shut-off of R* signalling is then accomplished by ... membrane hyper-polarization and the sensation of light (reviewed in [1]). Thesensitivity of the system requires that the signal transmitted by R* be rapidly attenuated. This is accomplished by a ... asymmetric unit of bothcrystal structures [9,10] has a radius of gyration of almost4.3 nm, and would have a forward scattering approximatelytwice as large as that observed by us. Neither of...
- 9
- 492
- 0
Tài liệu Báo cáo khoa học: Structural and functional specificities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx
... expression of highly speci-fic proteases that proteolytically activate the PDGFswill also influence the availability and activity of the dif-ferent isoforms. This can be exemplified by the proteo-lytic ... interstitial cells in a diversity of renal diseases butnot in normal human kidneys. Most interestingly,PDGFR-a is extensively expressed by interstital cells of normal kidney and the expression is further ... coronary arterysmooth muscle cells and fibroblasts [25,26]. Addition-ally, the CUB domain of PDGF-C exhibits mitogenicactivity on human coronary artery cells independent of the presence of its...
- 19
- 557
- 0
Tài liệu Báo cáo khoa học: Changes in purine specificity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx
... withreplacement of this segment by the rPDE2 region,which comprised only 23% of the residues of thecyaB1 GAF B domain, was exclusively, yet weakly, sti-mulated by cGMP, whereas the activation by cAMPwas ... lm, similar to wild-type cyaB1, and, surpris-ingly, activation by cAMP was eliminated (Fig. 2A,Table 1). Obviously, exchange of this region switchedthe specificity of the cyaB1 tandem GAF domain ... switch in purine specificity, only 11% of the sequence of thecyanobacterial GAF B needs to be replaced by PDE2 sequences. We wereunable, however, to switch the purine specificity of the PDE2 tandem...
- 10
- 468
- 0
Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDENghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Thơ nôm tứ tuyệt trào phúng hồ xuân hươngChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ