... was then cloned into the corresponding sitesbehind the galactose-inducible GAL1 promoter of pYES2(Invitrogen) to yield the plasmids pYDESA, pYDESB, pY-DESI, pYDESO, and pYDESK. The fidelity of ... for enzymes involved in the synthesis of EPA and DHA [18]. However, rather surprisingly, the first of these genes to be functionally characterized wasfound to encode a cytochrome b5 fusion desaturaseexhibiting ... werecloned into the vector pYES2, under the control of aninducible galactose promoter, to produce the constructspYDESA, pYDESB, pYDESI, pYDESK and pYDES-O. They were first expressed in the Saccharomyces...