... pyruvate, and meth-ylamine are bound to the enzyme in this order, andN-methyl-l-alanine and then NADP+are released from the enzyme. Therefore, the kinetic mechanism of the enzyme is the same ... substrate and was distinctfrom alanine dehydrogenase [18] and N-methylalaninedehydrogenase found by Lin & Wagner [17]. Lysineand ornithine were inert as an amine substrate; the enzyme ... HgCl2, and 160 lm forCoCl2. Carbonyl reagents, including hydroxylamine,phenylhydrazine, semicarbazide, and aminoguanidine,did not inhibit the enzyme. Chelating agents, such aso-phenanthroline...