Tài liệu Báo cáo khoa học: Isoprenoid biosynthesis via the methylerythritol phosphate pathway Mechanistic investigations of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase ppt
Tài liệu Báo cáo khoa học: Isoprenoid biosynthesis via the methylerythritol phosphate pathway Mechanistic investigations of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase ppt
... 4457 Isoprenoid biosynthesis via the methylerythritol phosphate pathway Mechanistic investigations of the 1-deoxy-D-xylulose 5 -phosphate reductoisomerase Jean-Franc¸ois Hoeffler, Denis Tritsch, Catherine ... this hypothesis, the influence of the concentrations of NADP+and MEP 5 ,the substrates of the reverse reaction, and of NADPH and DXP3, the products of the reaction, on the total amount of NADPH ... 2-C-methyl-D-erythrose-4 -phosphate 4: the role of NADPH The conversion of DXP 3 into MEP 5 by the DXR requires the presence of NADPH as cofactor. The first step of thisconversion, i.e. the rearrangement of DXP...
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Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf
... several molecular pathways. The best characterized and the most prominent path-ways are the extrinsic and intrinsic pathways. In the intrinsic pathway (also known as the ‘mitochondrial pathway ), apoptosis ... decreasing the activity of caspase-3 in the three cell lines.Effect of HSS knockdown on CCCP-inducedapoptosisTo further elucidate the functional role of HSS inCCCP-induced apoptosis, the expression ... opening of the nonselective, highly conductive PTP in the mitochon-drial IM [39]. The exact molecular composition of the PTP remains unclear. When MPT occurs, MPcollapses, leading to the failure of...
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Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt
... in the central cylinder of the root, but not in the lower parts of the hypocotyl (a). Rosette leaves werestained in the vascular tissue of the leaf blade, whereas the petiole and older parts of ... staining within the vascular tissue at the end of the leaf blade, whereas the basal parts close to the petiole remained almost unstained. We observed clearGUS expression in the primary root of 7-day-old ... leaves and in the anthers of dif-ferentiated flowers. We also detected meagre expres-sion along the root, with most obvious stainingpresent at the lateral root primordia and the base of the lateral...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... 1. The 18 kDa proteinpresent in the elution pattern was identified as the PHIcomponent on the basis of its ability to complement the Fig. 1. SDS/PAGE of PHI at different steps of purification. The numbers ... equilibrium. As shown in the inset, the progressive decrease of the molecular ellipticity atk ¼ 200 nm (the absorption region of the peptide bond)reflects the occurrence of a transition between ... PHO (abc) protomer. The hypothesis of adirect PHI–phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter the emission spectrum of PHI. Moreover, PHI...
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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx
... signaling pathways, such as the ERK1 ⁄ 2 pathway and the Akt pathway, as a conse-quence of inactivation of the EGFR [12]. With refer-ence to Fig. 1, which presents an overview of the intracellular ... inhibi-tion of Akt- and ERK1 ⁄ 2-dependent pathways eventu-ally change the expression level of one or more of these Bcl-2 family members.BadBad is one of the ‘death-promoting’ members of the Bcl-2 ... and the phos-phorylation state of their proteins, thereby changing the amount and localization of Bcl-2 family members.However, each type of cancer has its own way of disrupting the balance of...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... that argued against the existence of the complex and channeling of metabolites [10,11]. Thus, the existence of the com-plex has not been universally accepted. In addition to the cytoplasmic compartment, ... reducedby the coexpression of citrine–GAPDH, but coexpres-sion of PGK did not affect the lifetimes. Coexpression of citrine–GAPDH and PGK weakly decreased the lifetimes of cerulean–GAPDH in the cytoplasm, ... insert(B). The fluorescence decay curves of cerulean (blue) and citrine (green) representan average of fluorescence decay dataobtained from the cytoplasmic area of the observed cells. The decay curve of separately...
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Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx
... obesity.Experimental proceduresGeneration of H-PGDS TG mice The coding region of human H-PGDS was cloned into the downstream sites of the chicken b-actin promoter and the CMV enhancer of the pCAGGS expression vector ... analysisTo further assess the effect of H-PGDS overexpressionon the increase in adipose tissues, the weights of subcutaneous and visceral adipose tissues, as well as of muscle, of WT and TG (S55) ... amounts of PGD2in WAT after6 weeks of the HF diet. WAT of TG (S55) mice con-tained significantly more PGD2(approximately seven-fold) than that of WT mice (Fig. 3A). To examine the effects of the...
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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc
... just the result of the larger ionic radius of Cd(II)over Zn(II) and that the difference in amino acidsequence plays a role.One of the most important aspects is the greaterdynamics of the Cd(II)3-CysS9cluster ... in the pro-tein. The modification of the surface-accessible sulfur of cysteine ligands is thought to be the key thatunlocks the metals from the protein [38]. Thus, in gen-eral, reactions of the ... is a soft ligand andtherefore shows a preference for soft metals. More-over the structures of metallothioneins are not rigidand hence there is little selectivity concerning the size of the ion....
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Tài liệu Báo cáo khoa học: Simplified yet highly accurate enzyme kinetics for cases of low substrate concentrations ppt
... describe the function of the entire pathway [13]. This practice allows the investiga-tor to omit inessential complexity and to focus on the elements underlying the emergence of function of the pathway. ... phenomenon is offeredby an enzyme-catalyzed reaction where the function is the conversion of one metabolite into another: in thiscase, the formation and dispersion of the complex of the enzyme ... fastertimescale than the overall reaction of the metabolites,and thus the dynamics of the overall reaction can besummarized by the simpler enzyme kinetics. Indeed, at the level of a metabolic pathway such...
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Tài liệu Báo cáo khoa học: Brain angiogenesis in developmental and pathological processes: therapeutic aspects of vascular endothelial growth factor doc
... from the Ministry of Education, Culture, Sports, Science andTechnology of Japan, a grant from the program‘Research for the Future’ of the Japan Society for the Promotion of Science, and the ... vessels andtheir transfer to distant areas in the brain via bloodflow; (b) the rapid migration of tumor cells outsideblood vessels; and (c) the migration of tumor cellsindependent of the vascular ... rescue the ischemic and stillviable region of the brain called the ‘penumbra’ [14].Therefore, degree of angiogenesis appears to correlatewith rate of recovery from stroke.A variety of angiogenic...
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