Tài liệu Báo cáo khoa học: Medium-chain dehydrogenases/reductases (MDR) Family characterizations including genome comparisons and active site modelling pdf

... characterizations including genome comparisons and active site modelling Erik Nordling1,2, Hans Jo¨ rnvall1 and Bengt Persson1,21Department of Medical Biochemistry and Biophysics and 2Stockholm ... TheLTD active sites all are deep and narrow (Table 2). The active site is polar in all cases but one, and for the majorityof the LTD members, several charged residues are present atthe active site. ... used in genome annotations.Keywords: medium-chain dehydrogenases/reductases; genome comparisons; polyol dehydrogenase; cinnamylalcohol dehydrogenase; quinone oxidoreductase. Medium-chain dehydrogenases/reductases...

Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... is the same in both proteins, including a shortsegment (N56, C57 and D58) forming a turn of 310helix (D75, C76 and D77 in a-sarcin).The active site The active site is composed of well-deﬁned ... acid and a base on each side of the hydrolyzed bond [16].E96 and H137 in a-sarcin and E58 and H92 in RNaseT1 act as the base and acid groups, respectively. Com-paring the 3D structures, E66 and ... structure of the HtA active site, and the interactions between side chains, we proposethat D40, H42, R95 and F126 also form part of it. Asin other ribotoxins, the active site of HtA is buried,with...

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... KIND and PTPN13 KIND(Fig. 6A). The amino acid sequences of the coreregions of mouse, human and Gallus v-KIND shared65.6% homology, and 17 identical and four function-ally similar residues, including ... dendrites and soma, although not to axons. The expression of threeother KIND2-containing constructs (DKIND1, DRasN and DGEF) was restricted to dendrites and soma(Fig. 1B). On the other hand, two ... structural and functional properties of the KIND domains remainlargely unknown.Within the KIND protein family, v-KIND is uniquebecause it possesses two tandem-repeated KINDdomains, KIND1 and KIND2,...

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... several phosphorylated sites, including Thr69 and Ser87, and its degradation is promoted throughdephosphorylation of these sites. Ser70 is the majorphysiological phosphorylation site for the survivalfunction ... phosphorylation of the MAPK family and mitochondrial proteins. Phosphorylated ERK preventsthe activation of caspases and the Bcl-2 family, whereas these are activated by phosphorylated JNK and p38 MAPK, leading ... activation and regulate executionercaspases [43].Caspases are initially synthesized as inactive zymo-gens, and their dimerization is crucial for stabilizingthe conformation of the active site, ...

Tài liệu Báo cáo khoa học: RMI1 deﬁciency in mice protects from diet and genetic-induced obesity pptx

... homeostasis disorders [6]. Withthis method, it is possible to mutate the mouse genome randomly on a large scale, and then isolate and identify the mutated gene. Several other genes havebeen identiﬁed ... this mutant strain as described below). Body and organ weights as well as plasma parameters(Tables S2 and S3) were measured, and learning, mem-ory and behavioral tests (Table S4) as well as histo-pathological ... glucose and a reduced body weight (normal diet) wereobserved in the mutant mice. When fed a high-fat diet, the mutant micewere resistant to obesity, and also showed improved glucose intoleranceand...

Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc

... Green PCR Core Kit (Bioke, Leiden, TheNetherlands), 3 pmol of each primer (Isogen, De Meern,The Netherlands or Biolegio, Nijmegen, The Netherlands) and 3 lL of cDNA template (equivalent to 1 ng ... work ofBM Bakker and HV Westerhoff is further supportedby STW, NGI-Kluyver Centre, NWO-SysMO, BBSRC (including SysMO), EPSRC, AstraZeneca, and EUgrants BioSim, NucSys, ECMOAN, and UniCellSys.The ... substrates, products and effectors (X), and by changes in the afﬁnities(1 ⁄ K) of enzyme e towards its substrates, products and effectors (K). As derived previously [6,7], geneexpression and metabolic...

Tài liệu Báo cáo khoa học: Hsp105b upregulates hsp70 gene expression through signal transducer and activator of transcription-3 pdf

... & LatchmanDS (1999) Signal transducer and activator of transcrip-tion-1 and heat shock factor-1 interact and activate thetranscription of the Hsp-70 and Hsp-90beta genepromoters. J Biol Chem ... the basis of their apparent molecular weight and function, such as Hsp105 ⁄ 110, Hsp90, Hsp70, Hsp60,Hsp40, and Hsp27. The Hsp70 family is the major and best-characterized group of heat shock ... 2009)doi:10.1111/j.1742-4658.2009.07311.xHsp105a and Hsp105b are mammalian members of the Hsp105 ⁄ 110 family, a divergent subgroup of the Hsp70 family. Hsp105a is expressed constitu-tively and induced by various forms...

Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

... a(encoded by CKA1) and a¢ (encoded by CKA2), and two regulatory b subunits, b and b¢ in Saccharomycescerevisiae (CKB1 and CKB2). The enzyme occurs in allpossible combinations of a and b subunits ... cycle and highly resistant to proteolytic degradation [40].Miscellaneous proceduresYeast were grown on standard media [41] and transformedas described by Gietz et al. [42]. Microscopy and cell ... Labou-esse M, Minvielle-Sebastia L & Lacroute F (1991) A family of low and high copy replicative integrative and single-stranded S. cerevisiae ⁄ E. coli shuttle vectors.Yeast 7, 609–615.39...

Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... stimula-tory and inhibitory G protein and predominant localiza-tion of the endogenous CT receptor ganglioside GM1[28,29]. This would facilitate interactions between CT,GM1 and Gsa and, potentially, ... variousother tissues and cells has established both a cytosolic and membrane-bound localization [38], and over-expressed ARF-6 has been localized to the plasmamembrane and endosomes [39]. In ... is rapidly and speciﬁcallyinternalized with its ligand to low-density endosomes.Experimental proceduresPeptides, antibodies, protein determination,enzyme assays and materialsCT-A and CT-B,...

Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc

... withmethylglyoxal and diacetyl. In the oxidative reaction,the enzyme was active with glycerol and to a lowerTable 1. The specificities and kinetic properties of the histidine-tagged and purified GLD1 and GLD2. ... and gld2, were cloned and expressed ina heterologous host. The encoded proteins were purified and their kineticproperties characterized. GLD1 catalyses the conversion of d-glyceralde-hyde and ... afternucleotides 327, 510 and 916 of the open readingframe and contained 70, 69 and 62 nucleotides,respectively. The sequence of the open reading framecodes for a protein with 331 amino acids and a calcu-lated...

Xem thêm