Tài liệu Báo cáo Y học: The Ikaros family protein Eos associates with C-terminal-binding protein corepressors pptx
Tài liệu Báo cáo Y học: The Ikaros family protein Eos associates with C-terminal-binding protein corepressors pptx
... The Ikaros family protein Eos associates with C-terminal-binding protein corepressors Jose´Perdomo and Merlin CrossleyDepartment of Biochemistry, G08, University of Sydney, NSW, Australia Eos ... early stages of differentiation and to the lymphoid compartment in the adult [2,3]. The Ikaros genecodes for a protein with six zinc fingers that comply with the Kru¨ppel C2-H2 consensus. The ... birth [2]. The severity of the DN–/–mutation suggeststhat this aberrant Ikaros protein, which cannot bind DNA,is still able to dimerize with other Ikaros family proteinsand, most likely, interfere...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... efficient stereoselective synthesis of enantiomericallypure mono- and di-O-hexadecyl-b-D-glucosylglycerol ethers byepoxidation of an allyl b-D-glucopyranoside asymmetricallyinduced by the glucide ... inhibitors such as the epoxide 1d wouldinteract preferentially with the glucose low-affinity site. To the con-trary, the C-1 epoxypropyl analogue 3a might react in the high-affinitysite. The inactivation ... The oxidation of primary trimethylsilyl ethers toaldehydes: a selective conversion of a primary hydroxy group intoan aldehyde group in the presence of a secondary hydroxy group.J. Prakt. Chem....
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... Based on their derivedamino acid sequence, the corresponding enzymes have beenassigned to family 53 of the glycosyl hydrolases [16]. Theseenzymes have a retaining mechanism and for the Pseudo-monas ... performed with a Voyager-DE RP Biospectrometry Workstation(PerSeptive Biosystems, Framingham, VS) in the positivemode. The laser intensity was 2300, the pulsed delay timewas 200 nsec, the accelerating ... previously described [24]. Sequenceanalysis was performed on both strands of DNA by using the Cy5 AutoCycle Sequencing kit (Pharmacia Biotech,Uppsala, Sweden). The reactions were analyzed with...
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Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
... specific enzymes thatare involved in the biosynthesis of these structures and maylead to the discovery of potential therapeutic targets. Inaddition, the correlation of carbohydrate structure with ... conclusively demonstrate the linkage of the capsule polysaccharide to its membrane anchor and arecurrently trying to determine the nature of the membranelinkage.It is currently unknown whether the ... A.M., Wu, Y. , Young, N.M. & Wakarchuk, W.W.(2000) Biosynthesis of ganglioside mimics in Campylobacter jejuniOH4384. Identification of the glycosyltransferase genes, enzymaticsynthesis of...
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Tài liệu Báo cáo Y học: The RuvABC resolvasome Quantitative analysis of RuvA and RuvC assembly on junction DNA doc
... involves twokey steps: branch migration, catalysed by the RuvB protein that is targeted to the Holliday junction by the structurespecific RuvA protein, and resolution, which is catalysed by the RuvC ... efficiency. Also,E. coli RuvA has the ability to bind to the duplex arms of the Holliday junctions after specifically binding to the Holliday junction at the crossover point.From the analysis of the ... stoichiometry of the interaction. Thismethod has been used previously to study protein DNAinteractions [22,23].To calculate the stoichiometry of the interaction of RuvA with the Holliday junction, the...
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Tài liệu Báo cáo Y học: The effects of ring-size analogs of the antimicrobial peptide gramicidin S on phospholipid bilayer model membranes and on the growth of Acholeplasma laidlawii B ppt
... provide further support for the hypothesisthat the primary target of these antimicrobial peptides is the lipid bilayer of the bacterial membrane. The very high anti-microbial activity of GS14 ... rmcelhan@gpu.srv.ualberta.caAbbreviations: GS, gramicidin S; Myr2Gro-PCho, dimyristoylglycerophosphocholine; Myr2Gro-PEtn, dimyristoylglycerophosphoethanolamine;Myr2Gro-PGro, dimyristoylglycerophosphoglycerol; DSC, differential ... the thermotropic phase behavior of LMVs composed ofdimyristoylglycerophosphocholine (Myr2Gro-PCho), dimy-ristoylglycerophosphoethanolamine (Myr2Gro-PEtn) anddimyristoylglycerophosphoglycerol...
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Tài liệu Báo cáo Y học: The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex potx
... to the proteasome [14]. The self-compart-mentalization would thus protect the cell from uncontrolledproteolysis. This agrees with the observation that the enzyme is only fully active in the ... to know whether the human enzymenever associates or whether it transiently associates andthen dissociates. In general, the total amount of immuno -reactive protein obtained from the human clone ... a glycine in the murinesequence. As Gly252 is conserved in all sequenced variants ofTPP II, the recombinant enzyme with Arg252 is atypical.Nevertheless, as Arg252 evidently interferes with...
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
... envelopes changes verydynamically d epending on the stage o f the cell cycle. T oensure the p recise assembly/disassembly of nuclear envel-opes in t he cell c ycle, the binding of proteins on nuclearenvelope ... through the RS region, not the NM- andSK-regions. The directions of the changes in the affinityof NK and chromatin on treatment with the twocytosol fractions in vitro, i.e., increasing with a syntheticphase ... mixture. The nu mber was determined bycounting with a hemacytometer.Preparation of a synthetic phaseXenopusegg cytosolXenopus eggs were collected, dejelled, and then lysed toprepare a synthetic...
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Tài liệu Báo cáo Y học: The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays) pot
... pHmedia is favoured by ATP. They also found the blue shift in the absorption maximum of the photoconverted enzymeoccurring under these conditions to be inhibited by ATPand by the protein phosphatase ... Deyashiki, Y. , Hara, A.& Mitsui, Y. (1998) Crystal structure of the ternary complex ofmouse lung carbonyl reductase at 1.8 A˚resolution: the structuralorigin of coenzyme specificity in ... respect-ively, of the same enzyme [1,6–8] and Ryberg, Sundqvist andtheir coworkers [9–11] have recently reported resultssuggesting that this aggregation may be favoured by PORphosphorylation. The...
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Tài liệu Báo cáo Y học: The Fc receptor c-chain is necessary and sufficient to initiate signalling through glycoprotein VI in transfected cells by the snake C-type lectin, convulxin ppt
... recognitionof the receptor by the snake venom. This illustrates the dualmechanism of the complex, with one subunit, namelyGPVI, responsible for binding to the ligand, and the othersubunit, the FcR ... investigate whether GPVI was functional, we measuredtyrosine phosphorylation of the FcR c-chain and the tyrosine kinase Syk, which is tyrosine phosphorylated andactivated early after GPVI engagement. ... to the tyrosine kinase.Further, K562 cells stably transfected with only GPVI, andtherefore lacking FcR c-chain (K562/F1), did not show adetectable increase in tyrosine phosphorylation of Syk...
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