... the sequences of 1CEV and 1RLA and pasted into the
structural alignment. The alignment was then corrected by
hand. Since the sequences of the two arginases and
agmatinase greately differ in the ... in the case of E. coli
agmatinase, of the loop indicated by the letter a.
Fig. 5. Scheme of the binuclear manganese cluster and the localization...
... spectroscopy [17].
To compar e the mechanism of h ydrolysis of IPU and o ther
GH family 49 dextranases, the a nomer configuration of the
hydrolysate of IPU was determined. A polarimetric assay
was ... respectively, were used. The protein
concentration was measured by the method of Lowry
et al. with B SA as standard [37].
TLC
TLC was p erformed to analyse t he hydrolys...
... soybean [18], cowpea
[19], and poppy [20,21].
In the present study, the first insights into the secon-
dary and tertiary structure of PLD a2 from cabbage
are given. The surface structure of the ... effect by calcium ions.
Destabilization of PLDa2 by calcium ions was also
indicated by the proteolytic sensitivity of PLD a2 [34].
Calcium-induced decrease of sta...
... Insights into the interaction of human arginase II with
substrate and manganese ions by site-directed
mutagenesis and kinetic studies
Alteration of substrate specificity by replacement of Asn149
with ... molecule, by lowering the
pK
a
for proton ionization and generation of the
hydroxide that nucleophilically attacks the guanidino
carbon of the scissile...
... exchange of the H
A
-hydrogen of IPP. In
both cases, scrambling of the label takes place within the IPP
pool and the error is then transcribed into the DMAPP pool by
the normal action of the isomerase. ... exchange (2% of the
isomerization rate) of the methyl group of IPP and the
(Z)-methyl group of DMAPP, and an even slower exchange
(0.5% of t...
... fact, the bulk
of the proteolytic processing of the proforms of the
SVMPs occurs in advance of release of mature secre-
tory vesicle contents into the lumen of the venom gland.
In the case of the ... 3017
proteins are synthesized in the cytoplasm of secretory
cells in the gland and transferred to the rough endo-
plasmic reticulum, and then the Gol...
... at the rigidity of these non-projective
constructions and their best projective approxi-
mation possible by reordering. Rigidity here is
the reorderability of the constructions retaining
the ... in
HyDT, by definition, represents a set of adjacent
words which are in dependency relation with each
other, and are connected to the rest of the words
by a single incomin...
... . The
X-ray crystal structure 1 pi2 reveals that the /,w angles of
most of the residues of the Bowman±Birk inhibitor are
distributed fairly evenly over the b- and PPII-regions of the
Ramachandran ... strands, they would be expected to readily undergo this
type of aggregation w ith each other and a lso with the edges
of established bsheet. The m ore d ynamic typ...
... pathways from the synthesis of 3-ketoacyl
CoAs by a condensing enzyme to the incorporation of fatty
acids into lipids such as triacylglycerol or membrane
phospholipids. This in vivo system is therefore ... Elonga-
tion of [1-
14
C]18:1CoA to [3-
14
C]20:1CoA and
[5-
14
C]22:1CoA by the microsomes was measured by
incubation with malonyl CoA and required cofactors,
follo...
... hypothetical
second site have been made here for the bacterial enzyme by
stripping subunits III and IV off the native complex, and by
further destroying a large part of the acidic lobe(s) of the
docking ... complex of the
respiratory chains of mitochondria and many bacteria
[1–4]. It catalyzes the reduction of oxygen to water, coupling
the free energy of th...