Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf

... that of C. jejuni. In summary, we have presented the complete structures of the LOS and capsule polysaccharide of the genome sequenced strain C. jejuni NCTC 11168. The outer coreLOS of NCTC 11168 ... core and 6-O-Me-Hep in the capsule polysaccharide in this study. We are currentlycharacterizing the heptose biosynthetic pathways in NCTC 11168. The sugar composition of the NCTC 11168 polysaccha-rides ... C. jejuni serotype HS:3, and the poly(tetra-glycosylphosphates) from C. lari [6].Analysis of the genome sequence of C. jejuni NCTC 11168 revealed that the strain possessed a type II/III capsule locus...

Tài liệu Báo cáo khoa học: Isochore structures in the chicken genome ppt

... wereexcluded from the study. Our analysis of the distributions of G + C content, CpG islands, and genes was onlyrestricted to the remaining 24 chromosomes. The densities of CpG islands and genes were ... have its genome sequenced. Like most birdspecies, the chicken has a relatively small genome of $ 1200 million base pairs, or $ 39% of the size of the human genome [1]. The nuclear genomes of vertebrates ... obtained here are exactly the boundaries of the related regions. For example, thereis an abrupt increase (decrease) of the density of CpGislands at the ﬁrst (second) boundary of the shortGC-rich...

Tài liệu Báo cáo Y học: Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase ppt

... thesecompounds and the absence of synthesis of poly(A) by the E.colipoly(A) polymerase, we did not observed adenyly-lation of guanosine, GDP or Gp4G by the yeast enzyme.In the absence or presence of these ... as effectors of the synthesis of poly(A)Based on the above results, the effect of several diguanosinepolyphosphates on the synthesis of poly(A) was comparat-ively studied. The enzyme was incubated ... independent synthesis of poly(A)In order to understand why dinucleoside polyphosphatesactivated the primer independent synthesis of poly(A), the effect of 0.01 mMGp4GorAp4A on the synthesis of poly(A)...

Tài liệu Báo cáo Y học: CK2btes gene encodes a testis-speciﬁc isoform of the regulatory subunit of casein kinase 2 in Drosophila melanogaster potx

... phosphorylation activity of the CK2 a subunit[39]. The functional homology of the Stellate protein with the CK2 b subunit rises the f ormal possibility t hat the Stellate protein may take part in the ... mM).Fig. 3. Eﬀect of polylysine on the phosphorylation of calmodulin bycatalytic subunit or by holoenzyme reconstituted from CK2a and CK2btes proteins. Calmodulin was phosphorylated in the presence(lanes ... possesses catalytic activity and the regula toryb subunit was shown to enhance stability of the holoenzyme,activate CK2a and provide substrate speciﬁcity and CK2targeting in c ells. In spite of CK2b...

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... posi-tioned in the connecting loops. Approximately one-third of all mutations in the BTK gene are missense and some of these reduce the stability of the protein.This is exemplified by mutations in the ... extremely low and often undetectable in immunoblot analysis. The activation of CD4+cells, however, dramaticallyinduces transcription of the ITK gene and is key for the productive infection of HIV ... influences the behavior of the fusion proteins. Itis also possible that the differential expression of SRCfamily members in these two cell types may influence the phosphorylation levels of BTK–SYK....

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... complexity of the purification procedure. Moreover, as with the studies by Lee et al. [21] and Subramanian and Shree[42], there was no rigorous assessment of the purity of the product or the correctness ... affinity chromatography and subsequent throm-bin cleavage [41]. The use of thrombin significantlyincreases the cost of the purification, and the require-ment for HPLC increases the length and complexity ... the use of Ni-NTAaffinity chromatography for purification and subse-quent liberation of Ab by digestion using yeast ubiqu-itin hydrolase, but the authors did not provide data on the purity of...

Tài liệu Báo cáo Y học: Studies into factors contributing to substrate speciﬁcity of membrane-bound 3-ketoacyl-CoA synthases pot

... pathways from the synthesis of 3-ketoacylCoAs by a condensing enzyme to the incorporation of fattyacids into lipids such as triacylglycerol or membranephospholipids. This in vivo system is therefore ... we also noted the level of activity found inmany of the chimeric enzymes. Many of the changes wemade resulted in chimeric enzymes with substantiallylower activity than the wild-type parents. ... the desaturases to the corresponding hydroxylase residuesresulted in the conversion of desaturase activity to hydroxy-lase activity. The reciprocal experiment allowed the conver-sion of a hydroxylase to...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... they arehydrophobic and negatively charged. A comparison of rhodamine uptake, in the presence and the absence of uncouplers, allows the estimation of the effect of intracellular binding on the ... measures the total dye amount associatedwith the cells, including the dye adsorbed to the sur-face of the cells.To avoid complication of the uptake studies byactive pumping of the dyes into the ... avariety of ‘sensitive’ human cell lines exhibit MDRactivity capable of preventing the accumulation of rhodamine dyes in the cell cytoplasm. These cationic,relatively hydrophobic dyes, especially...

Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

... committed step, the phosphorylation of the c-carboxyl group of aspartate, of the biosyntheticpathway of the aspartic acid group amino acids Lys,Thr, Ile, and Met, in microorganisms and plants. ... domain of TtAK contributes not only tocatalytic regulation, but also the thermostability of TtAK. Comparison of the crystal structures of TtAKb and CgAKb was expected to elucidate the mechanism of ... mechanism of the elevated thermostability of TtAKb.In this article, we describe the crystal structures of TtAKb in two forms, Thr-bound and Thr-free, and discuss the regulatory mechanism of Thr and the...

Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc

... binding of the inﬂuenza virus tored blood cells via sialic acid [1], and later by work on the chemical basis of the antigenicity of polysaccharides and of the well-known ABO (H) blood-group system ... sequences and linkages of these oligosaccharides, were initially elucidated by high-sensitivity ES-MS/MS analysis, and then their full structureassignment was completed by methylation analysis and 1H-NMR. ... of diverse oligosaccha-rides, and more than 80 have been isolated and sequencesassigned [6]. Many of these structures are closely related to the carbohydrate chains of glycoproteins and glycolipids...

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