Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... betterclarify the occurrence of LOXs and their inﬂuence on hazelnut seed quality, we carried out the biochemical and molecular characterization of the main LOX isoformsexpressed during seed development. ... Characterization of hazelnut seed lipoxygenases (Eur. J. Biochem. 270) 4375 Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases Angelo Santino1, Angelo ... primers CA4 and CA5 (matching,respectively, from base 9488 to base 9520 and from base8366 to base 8387 of the sequence reported in Fig. 1) and Ó FEBS 2003 Characterization of hazelnut seed lipoxygenases...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... standard molecular weight proteins. The ﬂowrate was set to 0.8 mL Æmin)1. The subunit molecular masswas also estimated by SDS–PAGE. Characterization and comparative analyses of HYDJs and ... performed usingT-COFFEE [44].Docking simulation of substrate on the active site of HYDJsThe substrate binding pockets of HYDJs and the mode of interaction between substrate and enzyme were ... FEBS 3579 characterization and evaluation of HYDJsfromJannaschia sp. CCS1. Characterization of HYDJsTo explore the possible cause of the higher speciﬁcactivity for conversion of d,l-p-HPH...

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... members of the MAPEG superfamilya set of representative members were selected as seeds. Theseeds were the human member proteins of MGST1-3 (Uni-prot-Swissprot identiﬁers P10620, Q99735 and O14880);FLAP ... DNA and RNA were performed as describedpreviously [57].Isolation and cloning of the SynMGST and E.coliMGSTThe coding sequence for the SynMGST, corresponding tothe complementary strand of the ... al. Characterization of MAPEG membersFEBS Journal 272 (2005) 1688–1703 ª 2005 FEBS 1699BamHI and EcoRI and then the reformatted BamHI-EcoRIfragment was subcloned into BamHI and EcoRI sites of...

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... localization and associationwith the development of fruiting bodies. J. Gen. Microbiol. 125,147–157.328 S. Chen et al. (Eur. J. Biochem. 271) Ó FEBS 2003 Biochemical and molecular characterization of ... Kmvalue of 0.03 mM and a Vmax of 16.4ÆUmgprotein)1. Corresponding values for syring-aldazine and 2,6-DMP under optimal conditions were0.01 mM and 4.9 UÆmg protein)1, and 0.57 mM and 5.6 ... we havepuriﬁed and characterized one of the laccase isoforms,cloned and sequenced the cDNA encoding the enzymeprotein, and examined the effect of copper and variousCorrespondence to J.A. Buswell,...

Báo cáo khoa học: Biochemical and molecular characterization of puriﬁed chicken pancreatic phospholipase A2 docx

... ª 2009 FEBS Biochemical and molecular characterization of purifiedchicken pancreatic phospholipase A2Aida Karray, Fakher Frikha, Abir Ben Bacha, Yassine Ben Ali, Youssef Gargouri and Sofiane ... non-glycosylated monomeric protein with a molecular mass of 14 kDa and aspecific activity of 400 UÆmg)1in the presence of 1 mm sodium taur-odeoxycholate and 4 mm CaCl2with phosphatidylcholine ... more biochemical and structural properties of other purified bird PLA2s to gain more insights intotheir mode of action on phospholipids. We describe inthis study the purification and some biochemical...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... Department of Biology and Biochemistry, University of Bath, UK2 Department of Biochemistry, University of Wisconsin–Madison, USA3 Department of Chemistry, University of Wisconsin–Madison, USAIntroductionUpon ... studies of the structure and binding sites of enzymes. 8. Inhibitor binding toribonucleases. J Mol Biol 45, 491–511.33 Ui N (1971) Isoelectric points and conformation of proteins. I. Effect of urea ... December2010)doi:10.1111/j.1742-4658.2010.07976.x Molecular probes are useful for both studying and controlling the functions of enzymes and other proteins. The most useful probes have high afﬁnityfor their target, along with small size and...

Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc

... structure of cN-IIshowing interfaces A and B and the Mg2+site. The inset showsthe tertiary structure of each subunit. Effector sites 1 and 2 and the active site are shown.Table 2. Effect of point ... transfer of phosphate esteriﬁed in the 5¢ position of 6-hydroxypu-rine monophosphate nucleosides [1]. The transfer of phosphate can lead to phosphorylation of inosine,guanosine and a number of their ... behav-iour of recombinant wild-type enzyme in the presence of ATP and Mg2+as an activator and Pias an inhibitor.Figure 5 shows that the enzyme exists and functions as atetramer irrespectively of...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... four times.(A) PRTFDC1 and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine.Table 2. Km and Vmaxvalues for Hx and G in the presence of 1 mM PRPP, determined ... activity [10].The structural characterization of numerous com-plexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [13–17]. Thestructure of human HPRT can be divided ... domain is mainly builtup by residues from the C-terminus and consists of atwo-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig. 1A). Thetwo subunits...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... anaccurate measure of the koffparameter in CaM-boundeNOS and nNOS [58], and thus prevented assessment of the relative importance of conformational changerates versus rates of FMNH2formation ... Model and simulations of cytochrome c reduction by NOSenzymes. (A) Scheme of cytochrome c reduction. The model usesfour kinetic rates: dissociation (k1) and association (k2) of the FMN and ... in the nNOS and eNOSﬂavoproteins (dissociation and association of the4-electron reduced FNR–FMN subunit complex, kon and koffin Fig. 5). Remarkably, the results suggestthat koffis the...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: probes of hydrogen tunnelling pptx

... & BuckmannAF (1989) Enzymatic-synthesis of 4R-H-2NAD(P)H and 4S-H-2NAD(P)H and determination of the stereo-specificity of 7-alpha-hydroxysteroid and 12-alpha-hy-droxysteroid dehydrogenase. ... isotope effects using PEG-modifiedvariants and glycoforms of glucose oxidase: therelationship of modification of the protein envelopeto C–H activation and tunneling. Biochemistry 41,8747–8758.72 ... 3941MINIREVIEWStructural and mechanistic aspects of flavoproteins:probes of hydrogen tunnellingSam Hay, Christopher R. Pudney and Nigel S. ScruttonManchester Interdisciplinary Biocentre and Faculty of Life...

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