... AB0915 12. (Received 12 February 20 04, revised 27 April 20 04,accepted 30 April 20 04)Eur. J. Biochem. 27 1, 26 70 26 81 (20 04) Ó FEBS 20 04 doi:10.1111/j.14 32- 1033 .20 04.04195.x from bacteria [8] and ... couple, as predicted from the crystal structure of E. coli PGDH (Arg240, Asp264, Glu269 and His2 92) [18] were almost identical in EhPGDH (Arg217, Asp241 and Lys263), but Glu269 was substituted ... aligned among 35 PGDH and eight GDH sequences were selected and used for phylogenetic analysis. These correspond to theresidues 70– 121 , 130–159, 174 24 4, 25 7 26 1 and 26 3 28 7 of the E. histolytica...