Tài liệu Báo cáo khoa học: Analyzing changes of chromatin-bound replication proteins occurring in response to and after release from a hypoxic block of replicon initiation in T24 cells pptx

... 2003 Analyzing changes of chromatin-bound replication proteins occurring in response to and after release from a hypoxic block of replicon initiation in T24 cells Maria van Betteraey-Nikoleit, Karl-Heinz ... be induced to bear a maximalnumber of replicons arrested in the hypoxic preinitiationstate’ and as few as possible in other states of replication, and secondly the elaboration of a protocol ... soon as itsfunction in replication is required, after replicon initiation had taken place.Immunoﬂuorescence staining of total cellular and chromatin-bound PCNA T24 were grown and incubated...

Tài liệu Báo cáo khoa học: Animal models of amyloid-b-related pathologies in Alzheimer’s disease docx

... animal model in the same laboratory. Conven-tional tests are also time-consuming and greatly in uenced by individual handlers. IntelliCages areautomated learning cages where animals carryingtransponders ... understanding of molecular patho-genesis. Today a wide range of animal models areavailable for mechanistic, therapeutic and functionalstudies. They offer an appealing means to rapidlymove from ... DeMattos RB, Bales KR, Cummins DJ, Dodart JC,Paul SM & Holtzman DM (2001) Peripheral anti -A beta antibody alters CNS and plasma A beta clearance and decreases brain A beta burden in a mouse...

Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

... treatment of cells with thetransforming factor heregulin leads to increases in thelevels of HSF1 and MTA1 (a component of the NuRDcomplex containing HDAC1 and HDAC2) proteins and to binding of HSF1 ... of mam-malian HSP70 is hypersensitive to DNase I and contains paused polymerase [62–64]. Although GAGAfactor-like DNA-binding activity has been reported,the involvement of a mammalian GAGA ... transcription fac-tor IIA (TFIIA) [31], Kin28 (a C-terminal-domainkinase of TFIIH) [32], the Taf9 subunit of TFIID and Spt-Ada-Gcn5 acetyltransferase (SAGA) [33,34], and the Med17 and Med22 subunits of...

Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

... structural data of the R. capsulatus BchI and general functional aspects of AAA+ proteins [10]. In thismodel not only the BchI proteins are organized in an AAA+hexamer, but also BchD as the amino ... not suﬃcient to maintain the 70 kDa subunit in the cell. The ATPasedeﬁcient 40 kDa proteins fail to participate in chelation in a step after the association of the 40 and 70 kDa subunits.This ... mutant plants aresuggestedtolackexpressionoftheXAN-HproteinasnoXantha-h mRNA could be detected in these strains [24]. In contrast, the 70 kDa ChlD protein of Arabidopsis thalianaaccumulates to...

Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

... chromosome was ampliﬁed byPCR using chromosomal B. subtilis DNA as template and the oligonucleotides 5¢-TTGGTGGGATCCGTGACTCGAGCAGAACGAAAAAGAC-3¢ and 5¢-GGCTTTGTCGACTTATCGCACACTATAGCTTGATG-3¢ as primers(restriction ... currently avail-able antibiotics. Most notably, many strains are multidrugresistant, and the rapidly spreading resistance againstvancomycin and methicillin constitutes a life-threateningproblem in ... c-proteobacteria A. vinelandii and P. luminescens and to Idi-1 protein of the BacteroidC. hutchinsonii (expect values 3e-22, 5e-19 and 8e-20,respectively).Paralogs of Idi-2 proteins Database searches...

Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

... steel tray.The tray was then placed on top of a hot plate and thetemperature maintained at 9 0 °C for 5 min to aid thestaining of protein spots. The tray was then placed on a laboratory shaker ... models of neuromusculardiseases are an indispensable tool in modern myologyresearch. They are employed fo r studying the molecular and cellular factors leading to necrotic changes and in evaluatingnew ... gels.2D ‘Stains-All’ analysis of dystrophic muscleThe cationic carbocyanine dye ÔStains-AllÕ was u sed to determine potential changes in the expression of majorCa2+-binding proteins in dystrophic...

Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx

... 30041–30051.22 Murakami M, Yoshihara K, Shimbara S, Lambeau G,Gelb MH, Singer AG, Sawada M, Inagaki N, Nagai H,Ishihara M et al. (2002) Cellular arachidonate-releasingfunction and in ammation-associated ... Naiman BM,Wachtmann TS, Umland JP, Pandher K, Lapointe JM,Saha S, Roach ML et al. (2003) Impaired in ammatory and pain responses in mice lacking an inducible prosta-glandin E synthase. Proc Natl ... detected, in Fig. 2. Immunohistochemical localization of sPLA2-IIA in humanjoints affected by RA. Staining of sPLA2-IIA in an inactive RA tissuewas rare, and only a few synovial lining regions...

Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf

... Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, USASEA (sea urchin sperm protein, enterokinase and agrin) modules, minimally comprised of 120 aminoacids ... 5¢-GTTCAGGCCAGCATCTGTGGTGGTACAATTG-3¢ (sense), 5¢ -CAATTGTACCACCACAGATGCTGGCCTGAAC-3¢ (antisense),S ⁄ A substitution: 5¢-GTTCAGGCCAGGAGCTGTGGTGGTACAATTG-3¢ (sense), 5¢-CAATTGTACCACCACAGCTCCTGGCCTGAAC-3¢ (antisense), ... theWTF /A R /A UG /A V /A S /A 5075105160250UWTF /A R /A G /A S /A V /A 2505075105353025WTF /A R /A G /A S /A V /A WTF /A R /A G /A S /A V /A 751053025 A BCDFig. 2. Expression and release of wild-type and cleavage sitemutants of MUC1FDTR in...

Tài liệu Báo cáo khoa học: Identiﬁcation of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis docx

... mean ± SE from four independent determinations.Table 2. Subdomain boundaries of tACE and ACE2. The zinc prote-ase domain of both tACE and ACE2 is divided into two subdomains[20]. Subdomain ... chemiluminescence using an ECL kit. Fordensitometric analysis, data were captured using a FujiLAS-1000 Imaging System CCD camera (aida 2.11 soft-ware for analysis).ACE2 ⁄ ACE activity assaysFluorogenic ... subdomain II involved directly in substratebinding (Arg273 in ACE2 and Gln281, Lys511, Tyr520 in tACE, which bind the C-terminal carboxy group of the respective inhibitors) and catalysis (His505 in ACE2...

Tài liệu Báo cáo khoa học: "Automatic Evaluation of Machine Translation Quality Using Longest Common Subsequence and Skip-Bigram Statistics" doc

... large-scale machine translation evalua-tions. Recently, Turian et al. (2003) indicated that standard accuracy measures such as recall, preci-sion, and the F-measure can also be used in evalua-tion ... effectiveness as an auto-matic evaluation measure. To apply LCS in machine translation evaluation, we view a translation as a sequence of words. The intuition is that the longer the LCS of two transla-tions ... composite factors are LCS-based recall and precision in this case. Melamed et al. (2003) used unigram F-measure to estimate ma-chine translation quality and showed that unigram F-measure was as good...

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