Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa Comparison of twelve xylanases in relation to their thermal stability pdf
Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf
... effector-binding unit composed of the ACT1 and ACT2Fig. 5. Comparison of a single effector-binding unit between TtAKb-Thr and TtAKb-free. (A) Superposition of the effector-binding units of TtAKb-Thr ... the ACT2 domain; and (c) two ACT domains,each from a different chain, interact to form an effec-tor-binding unit. The effector-binding unit of theACT domain in the b-subunit of CgAK (CgAKb)isorganized ... densities of the N-termi-nal (residues 1–3 in chain A, residues 1–4 in chains B and C, and residues 1–5 in chains E–G) and C-termi-nal (residues 158–161 in chains A–F, and residues159–161 in chain...
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Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of specificity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf
... 279NADP+-binding pocket and restricts the bindingdepth of cofactor in a similar fashion to that observedfor OaPDH [12]. In TbPDH, the alanine in this motifis replaced by glycine suggesting less ... the cofactor-binding domain of LlPDH are strictly conserved in TbPDH, 89 in OaPDH. There are 17 residues within 4 A˚ of thecofactor, of which 15 are identical in the bacterial,trypanosomal and ... protein with ligands in a 1 : 5 ratio. Complex III was prepared by mixing the pro-tein, NADP+ and inhibitor in a ratio of 1 : 5 : 10. Crystalswere grown in hanging drops constructed from 2 lLofprotein...
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Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx
... towards dsDNA, ssDNA and plasmidwere analyzed using linearized pBAD ⁄ gIII plasmid, linea-rized and denatured plasmid and intact plasmid. ThepBAD ⁄ gIII plasmid was linearized using SalI and ... dena-tured by incubation at 98 °C in a PCR machine for 3 min, and then kept on ice. Approximately 300 ng of the varioussubstrates was mixed with 30 ng enzyme in a total volume of 20 lL containing 1 ... chromato-graphy was estimated to 0.8 m for VsEndA and 0.65 mfor VcEndA.Fig. 1. Sequence alignment showing the amino acids of VsEndA and Vc EndA. Numbers indicate cysteines involved in disulfide...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... usingcrotonyl-CoA as a substrate [15]. The gene encodingthis bifunctional protein was named AUH (‘AU bind-ing homolog of enoyl-CoA hydratase’). The RNA-binding activity of the human protein ... exon 7 and an inser-tion mutation c.613–614insA. This insertion causes aframeshift that starts at Met205 and leads to the intro-duction of a stop codon after four amino acids. Theintra-assay ... a substratefor testing AUH activity in a routine setting. Indeed,we were able to show that AUH activity in fibro-blasts can be determined by monitoring the forma-tion of 3-hydroxyglutaryl-CoA....
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Tài liệu Báo cáo khoa học: "Semantic Classification of Noun Phrases Using Web Counts and Learning Algorithms" ppt
... se-mantic relation. 2.2 Machine Learning Algorithms We are also interested in comparing the perform-ance of machine learning algorithms on the task of mapping from n-gram frequencies of joining ... learning algo-rithms consisted of vectors of web hits obtained using the 14 most frequent joining terms found in the BNC. The next condition used a vector of web hits obtained using the joining ... modifier and a “joining term” that links them. Some algorithm can then be used to map from joining term frequencies to semantic relations and so find the correct relation for the compound in question....
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Tài liệu Báo cáo khoa học: "Automatic Collection of Related Terms from the Web" pptx
... Automatic Collection of Related Terms from the WebSatoshi Sato and Yasuhiro SasakiGraduate School of InformaticsKyoto UniversitySakyo, Kyoto, 606-8501Japansato@i.kyoto-u.ac.jp, sasaki@pine.kuee.kyoto-u.ac.jpAbstractThis ... configurationAutomatic acquisition of technical terms in a cer-tain domain has been studied as automatic termrecognition (Kageura and Umino, 1996; Kageura and Koyama, 2000), and the methods ... “s toha”, “s toiu”, “s ha”, and “s”, where toha, ha, and toiu are Japanesefunctional words that are often used for defin-ing or explaining a term. Then, the system col-lects the top K (= 100)...
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Tài liệu Báo cáo khoa học: "AUTOMATIC ACQUISITION OF SUBCATEGORIZATION FRAMES FROM UNTAGGED TEXT" doc
... English, Hornby and Covey (1973) (e.g., DeMarcken, 1990). In either case, such lists are expensive to build and to main- tain in the face of evolving usage. In addition, they tend not to include ... out in Section 1.2. 2.1 SF DETECTION The obvious approach to finding SFs like "V NP to V" and "V to V" is to look for occurrences of just those patterns in the training ... with an infiniti- val complement example (1) showed that being followed by an infinitive is not the same as taking an infinitival complement. It might be interesting to try building a verb...
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Tài liệu Báo cáo khoa học: Isochore structures in the chicken genome ppt
... domains in DNA sequences can provide much insight into the understanding of theorganization and biological functions of the chicken genome. A new seg-mentation algorithm, which is simple and ... and Tnare the cumulative numbers of the bases A, C, G and T, respectively, occurring in thesubsequence from the first base to the n-th base in theDNA sequence inspected. Here, znis one of ... (decreasing) linearfunction of n. To amplify the deviations of zn, the curve of zn$ n is fitted by a straight line using the leastsquares technique,Isochores in the chicken genome F. Gao and...
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Tài liệu Báo cáo khoa học: "Different Structures for Evaluating Answers to Complex Questions: Pyramids Won’t Topple, and Neither Will Human Assessors" docx
... of combiningjudgments of nugget importance from multi-ple assessors increases the stability and dis-criminative power of the evaluation while in- troducing only a small additional burden in terms ... nuggets” to assess an-swers to complex questions. As it has become thede facto standard for evaluating such systems, theresearch community stands to benefit from a betterunderstanding of the ... both stability and discriminativepower. In Section 4, we discuss an alternative forcombining multiple judgments that parallels the dis-tinction between micro- and macro-averaging oftenseen in...
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Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa Comparison of twelve xylanases in relation to their thermal stability pdf
... 2003 Three-dimensional structures of thermophilic b-1,4 -xylanases from Chaetomium thermophilum and Nonomuraea flexuosa Comparison of twelve xylanases in relation to their thermal stability Nina ... higher protein density and lower void volumesindicating better packing. In the comparison of PDB structures, Karshikoff and Ladenstein [51] have observed that proteins from thermo-philic and mesophilic ... structures of CTX and NFXallowed us to make detailed comparison of 12 xylanases, five from thermophilic organisms. This gives a more reliable comparison of the enzyme structures in relation to...
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