Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt
... [6,7,9].
The < /b> interaction between Nop53p and < /b> Nip7p in the
< /b> two-hybrid system confirms the < /b> finding of these two
proteins in the < /b> pre60S complex. The < /b> two-hybrid system
was also used to test the < /b> interaction between ... oligonucleotide probes. The < /b> relative positions of the < /b> probes on the < /b> 35S pre-rRNA are indicated in
(B) . Bands co...
... isolated and < /b> analyzed by northern blot using Fluc and < /b> Rluc riboprobes. The < /b> radioactive signals were quantified by
the < /b> STORM 820 equipment and
< /b> IMAGEQUANT software (Molecular Dynamics). The < /b> Rluc ... [41]. The < /b> MS2 -CP (pCMS2) plasmid
was described previously [58]. The < /b> plasmids encoding
TTP -MS2 -CP (accession number: NP_035886), ASF ⁄...
... contrast, the < /b> ECD of BMPR-II was
not bound by proBMP-2. Furthermore, the < /b> free pro-
peptide formed a non-covalent complex with < /b> mature
BMP-2 in vitro, thereby blocking binding of BMP-2 to
BMPR-II. The < /b> ... interfere with < /b> binding
of the < /b> mature part to BMPR-IA. As the < /b> BMPR-IA
binding site for BMP-2 partially overlaps with < /b> the < /b> area
bo...
... inhibitor epoxomicin stabi-
lized RNF13 (Fig. 4B, lane 5), as did MG132 (Fig. 4B,
lane 4). Both the < /b> heterogeneous bands at 80 kDa
and < /b> the < /b> group of bands at 65 kDa were recognized
by the < /b> anti-HA ... but few endosomal
E3s have been characterized, and < /b> none has been shown
to be regulated by domain-specific proteolysis.
PA-TM-RING family members are pred...
... found on the < /b> cell membrane and < /b> in the
< /b> secreted fraction, as they represent the < /b> ‘face’ shown by
the < /b> bacterium to its host, thereby leading to its identifi-
cation and < /b> elimination.
Although TB is mainly ... constants and
< /b> maximum number of sites per cell were obtained from these curves. Inset: the < /b> abscissa is log F in the < /b> Hill plot...
... article with < /b> title “Franz Kafka” and
< /b> to expect the < /b> answer in the < /b> text on that page. For
the < /b> question “Who invented the < /b> automobile?” on
the < /b> other hand, it is more reasonable to search the
< /b> information ... answer can-
didates. (The < /b> numbers behind the < /b> terms indicate
query weights.) As can be seen, we initially build
two separ...
... example, “john subj
buy”, the < /b> analysis returned by m inipar for
John buys, is stored as “john buy john<subj
26
subj <buy john<subj<buy”. All words, de-
pendencies and < /b> partial dependencies ... ensure compatibility of query
and < /b> index formats.) The < /b> Lucene queries were
run on the < /b> three indexes. LBD disambigua-
tion was then app lied based on th...
... proteins of Fyn-SH2 and < /b> Fyn-
SH3 to further investigate the < /b> mechanisms by which
tau and < /b> Fyn interact. We determined that tau binds to
both Fyn-SH2 and < /b> Fyn-SH3, and < /b> only the < /b> former of
these associations ... proteins pulled down
by GST–Fyn-SH2 beads on western blots labelled with < /b> antibodies
against tau (V5) or GST. Numbers on the < /b> left...
... m
M
0 m
M
AB
CD
Fig. 3. Thermostability of PhyH determined at 20, 35 and < /b> 45 °C after incubation at 35 °C (A) or 45 °C (B) , and < /b> thermostability of PhyH (C)
and < /b> PhyH-DII (D) at 60 °C in the < /b> presence ... Coo-
massie Brilliant Blue R250. Protein concentration was
determined using the < /b> Bradford assay with < /b> BSA as the
< /b> standard [28].
Phytase activity...