Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc

... alkyla-tion leading to a depletion of mtDNA in intact cells(Fig. 1). Here we report the synthesis and characterization of a novel mitochondria- targeted alkylating reagent and show that it alkylates ... should also alkylate mtDNA within mitochondria and cells.MitoDC-81 does not alkylate mtDNA in isolated mitochondria Having ascertained that mitoDC-81 was sequestered byisolated mitochondria and ... plasma membrane potential and thenbe further accumulated within the mitochondria due to themitochondrial membrane potential [11]. From the knownplasma and mitochondrial membrane potentials and...

Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf

... molecular mass value of HAD was measured; instead of an adduct increase, orno change, the value had unexpectedly decreased from22 417.0 Da to 22 413.2 Da, a loss of 4 Da. MS ⁄ MS of these molecular ... causes a 1 Da increase in any fragment ion containing that residue. The average mass gain of N-terminal and C-terminal fragment ions are plottedas positive and negative, respectively, mass increases, ... 4-amino-5-hydroxymethyl-2-methyl-pyrimidine kinase and pyridoxal kinase. J Bacteriol186, 1571–1573.22 Xu G, Zhai H, Narayan M, McLafferty FW & ScheragaHA (2004) Simultaneous characterization of theTop-down MS of proteins...

Tài liệu Báo cáo khoa học: Efﬁcient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt

... clipping of a fragment of desired length and sequence from a nat-ural RNA may be a useful application. For example,RNA fragments that involve modiﬁed nucleobases areeasily obtainable from naturally ... this informa-tion is available, rational design has contributed to thedevelopment of new functional RNA, for example, sig-nalling aptamers and aptazymes [8–10].Work in our laboratory has focused ... proceeds withsimilar activity at both individual sites; cleavage ratesare virtually identical, ligation rate constants vary by a factor of only about 2.5.Alteration of RNA sequence by the twin...

Tài liệu Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells doc

... Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cellsAnthoula Gaigneaux, Jean-Marie Ruysschaert and Erik GoormaghtighLaboratory of Structure and ... The data were analysed with a window of ﬁvewavenumbers, assuming that adjacent wavenumbers arehighly corr elated. A population o f 3 2 solutions was built ateach generation, and e valuated. ... greatest interclass variance and the smallest intraclass variance, and constructs a linearcombination of the variables to discriminate between theclasses. The rule i s constructed with training...

Tài liệu Báo cáo khoa học: Membrane targeting and pore formation by the type III secretion system translocon pdf

... 213–222.66 Hamaguchi M, Hamada D, Suzuki KN, Sakata I &Yanagihara I (2008) Molecular basis of actin reorgani-zation promoted by binding of enterohaemorrhagicEscherichia coli EspB to alpha-catenin. ... Y, Sagara H, Kabe Y, Azuma M, Kume K,Ogawa M, Nagai T, Gillespie PG, Sasakawa C &Handa H (2007) The enteropathogenic E. coli effectorEspB facilitates microvillus effacing and antiphago-cytosis ... theplasma membrane and increasing Salmonella invasionefﬁciency [62]. It is of note that both IpaC and SipCare essential for Shigella and Salmonella uptake bymacrophages in the early steps of...

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... Sepuri, Sanjay Yadav, Hindupur K. Anandatheerthavarada and Narayan G. AvadhaniDepartment of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USACytochrome ... 86–93.8 Bhagwat SV, Biswas G, Anandatheerthavarada HK,Addya S, Pandak W & Avadhani NG (1999) Dual targeting property of the N-terminal signal sequence of P450 1A1 . Targeting of heterologous ... Niranjan BG, Raza H, Shayiq RM, Jefcoate CR &Avadhani NG (1988) Hepatic mitochondrial cytochromeP-450 system. Identiﬁcation and characterization of a precursor form of mitochondrial cytochrome...

Tài liệu Báo cáo khoa học: The most C-terminal tri-glycine segment within the polyglycine stretch of the pea Toc75 transit peptide plays a critical role for targeting the protein to the chloroplast outer envelope membrane ppt

... *SA*AAAAA*AGG AAA*******GAG ****AAA***GGA *******AAAGAA ****AAAAAAAGA AAA****AAAAAG AAA*AAA***GGD *******DDDGGE *******EEEGGL *******LLLGGN *******NNNGGS *******SSSGGP *******PPP A. J. Baldwin ... GGPprecursor to the supernatant and the membrane frac-tions was similar to those of GGN, GGD, GGS, and AAG (Figs 2B and 4B). Interestingly, similar to thecase of GGN mutant, a small but signiﬁcant amount of ... http://rsb.info.nih.gov/ij/) and shown as a percentage of the total amount of pre-cursor subjected to the import reaction. The mean values and standard deviations represented by error bars were calculated based on atleast...

Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer ppt

... C-terminal regula-tory domain. These provide docking sites for downstream molecules and lead to the evasion of apoptosis, to proliferation, to invasion and to metas-tases, all of which are important ... for a 170kDa receptor tyrosine kinase. AllERBB proteins have four functional domains: anextracellular ligand-binding domain; a transmembranedomain; an intracellular tyrosine kinase domain; and ... transducer and activator of transcription(STAT)3 and STAT5 pathways (Fig. 2) [3]. These even-tually result in cell proliferation, migration and metas-tasis, evasion from apoptosis, or in angiogenesis,...

Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR-targeted anticancer therapy doc

... lung can-cer with epidermal growth factor receptor mutations. BrJ Cancer 95, 998–1004.16 Sutani A, Nagai Y, Udagawa K, Uchida Y, KoyamaN, Murayama Y, Tanaka T, Miyazawa H, Nagata M,Kanazawa ... 3340–3346.15 Asahina H, Yamazaki K, Kinoshita I, Sukoh N,Harada M, Yokouchi H, Ishida T, Ogura S, Kojima T,Okamoto Y et al. (2006) A phase II trial of geﬁtinib asﬁrst-line therapy for advanced non-small ... wereobtained in a retrospective analysis of a trial of pani-tumumab in patients with mCRC [5]. Panitumumab, a fully human mAb targeted to the extracellular domain of EGFR, is of the IgG2 isotype, and...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing docx

... BishakhaShrestha, Saoni Mandal and other Mandal lab mem-bers for useful discussion and critical comments. Thiswork was supported in parts by ARP (00365-0009-2006) and American Heart Association ... geneactivation A widely studied NR coactivator is activating signalcointegrator-2 (ASC2, also named AIB3, TRBP,TRAP250, NRC, NCOA6 and PRIP) [69]. ASC2 is a coactivator of multiple nuclear receptors ... a DNA-binding domain, a ligand-bindingdomain and a transactivation domain. The DNA-bind-ing domain is responsible for DNA binding speciﬁcity and dimerization, and the ligand-binding domain...

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