Tài liệu Báo cáo khoa học: Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites doc
... concerning the kinetics and properties of the
enzyme. More strikingly, we show a novel role of the PPase
enzyme in the development and molting process of A. suum
larvae in vitro.
Materials and methods
Parasites
Adult ... important role in the development and
molting of the larval stage parasites. Furthermore, the
biochemical as well as...
... of Pi on the subtilisin digestion
indicates stabilization of the D-loop.
Monitoring the binding of cofilin to F-actin by
limited proteolysis
The effect of Pi on the binding of cofilin to F-actin
was ... according to our observations, the rate
of cofilin binding to F-actin in the presence of
2–30 mm Pi is the same at pH 6.5 and 8.0. The in u-
ence of Pi on...
... staining indicated that AtHELPS is mainly expressed in the young
seedlings and vascular tissues of leaves and roots. Using both helps mutants
and overexpression lines, we observed that, in the ... for the helps mutant and OE lines of AtHELPS. (A) The conserved
motifs of DExD ⁄ H-box RNA helicases in AtHELPS. Numbers represent the amino acid position of the At...
... suggesting that this peptide may also
affect the development of the wing disks at the prepu-
pal stages. It is interesting to note that bombyxin-II
also stimulated the growth of the wing disks in the
butterfly ... of the input and the eluate of the chromatography
were resolved by Tricine ⁄ SDS ⁄ PAGE and then analyzed by Coo-
massie Brilliant Blue staining (le...
... reaction with the cysteine ligands of zinc sites in proteins
and concomitant zinc release. Stoichiometric amounts of acrolein release
zinc from zinc–thiolate coordination sites in proteins such as ... phos-
phorylation signaling and gene expression.
Results
Aldehydes release zinc from zinc-binding proteins
The effect of aldehydes on the zinc-binding capacity of
zinc prot...
... human and several
other primates [32,34,36], pig [37] and guinea-pig [38],
and it has been lost in rat [39]. On the other hand,
the gene has an intact open reading frame in a distant
relative of the ... high stringency. Two BAC
clones were isolated, one of which was sequenced with
primers based on the original PCR clone and gave the
remaining part of the coding r...
... at its N-terminus
instead of a free amino group [32]. Therefore, the
asparagine in position 26 was labeled within the intact
protein at its a-amino group and represents the amino
terminal amino ... the amino terminus of the pro-
tein. Using this method, we showed that asparagine 26
represents the amino terminus of P40. Knowing, that
the first amino acids of the...
... models and bilingual lan-
guage models to identify high quality sentence pairs,
and use these sentence pairs in the SMT training. In
the remaining part of the paper, we introduce the ap-
proach and ... the validity of the actual
meaning of the sentence.
The set of SSR is a subset of the phrase table.
Therefore, the features in the phrase table can b...
... combining
assessor opinions, which yields a distinction
similar to micro- and macro-averaging in the
context of classification tasks. While the
two approaches differ in terms of underly-
ing assumptions, ... development and refinement of new
methodologies for evaluating and comparing sys-
tems. In the Text REtrieval Conference (TREC) QA
tracks organized by the U.S. N...
... biosyn-
thesis.
The process of binding and uptake of maternal lipopro-
teins is carried out by placental trophoblast [17], which lines
the chorionic villi and represents the epithelial layer
separating ... and 100 lgproteinÆmL
)1
) in the absence (total
binding) or presence of a 20-fold excess of unlabeled HDL
3
(nonspecific binding) [37]. Following this incubation t...