... respectively, in T. cruzigGAPDH) were selected for the following reasons. (a) Theyare involved in the two phosphate–anion binding sites:Thr225 in the Pi site (for inorganic phosphate-binding site)and ... GAPDH structuresavailable [16,18,19,22,24–29] and seems important to main-tain the correct positioning of the active residue Cys166 and the nicotinamide ring of the NAD+cofactor duringcatalysis. ... complex of T. bruceigGAPDH with an analogue of 1,3-BPGA, we chose toinvestigate the enzyme inhibitor interactions by studying the kinetics of enzymatic inactivation with the native proteinand...