Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc

Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane afﬁnity and hyper-anticoagulant activity in mouse plasma pptx

... regions containing over 20 grouped PS molecules).We have shown that doubling the PS concentration in the membrane results in an increase in the number ofbinding sites (compare Fig. 3D and 3E) and ... membrane-binding domain [9,10]. ThisCa2+binding requires the presence of Gla residues. The Gla domains within the protein C family comprise44 amino acids and contain between nine and 11 Glaresidues, ... utilizinga higher number of binding sites, mutants II and IIIgained their modest (approximately ten-fold) mem-brane binding afﬁnity enhancement by minor increases in the overall on-rate and ⁄...

Tài liệu Báo cáo khoa học: A novel nuclear DNA helicase with high speciﬁc activity from Pisum sativum catalytically translocates in the 3¢ﬁ5¢ direction docx

... preincubating PDH120 with the inhibitory concentration of all these inhibitors prior todilution in the unwinding reaction. Under these condi-tions, there was no inhibition of the unwinding ... polypeptide containing the properties of an antibiotic, intercalates into dsDNA and thereby inhibits nucleic acid synthesis [23]. Nogalamycin and daunorubicin are anthracycline antibiotics and areconsidered ... not they contain a fork. It translocates unidirectionallyalong the bound strand in the 3¢ﬁ5¢ direction. The enzymealso exhibits intrinsic single-stranded DNA- and Mg2+-dependent ATPase activity. ...

Tài liệu Báo cáo khoa học: Changes in purine speciﬁcity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx

... turnedinwards and interact with each other and with the a-helical linkers between the GAF A and B subdomains. In both structures, the cAMP-binding and cGMP-binding pockets are similar. A neighbouring ... GAF A and B bind cAMP (Fig. 1A,B,D,E). Again, the b1–b3region is the major interaction site for the adeninemoiety. In b1, an arginine residue (Ile417 in rPDE2)binds to N1 and a threonine (Ser419 ... Asp434 in rPDE2) that interacts with the pyrimidine ring of the purine, either individu-ally or in various combinations in 12 different con-structs, enhanced cAMP binding but had little effecton...

Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc

... mitochondrial proteins and ensures the elimination of damaged proteins [28]. This continuousFig. 3. Determination of CML-protein content in liver mitochondrial matrix with aging. The CML content ... polyclonal antibodyagainst Lon protease was raised in rabbit against a syntheticpeptide corresponding to amino acids 208–221 of rat Lon. This antibody mainly recognized one protein band with anestimated ... 10-month-old matrix proteins (lane 1)contained 14 bands intensely stained over an apparentrange of 10–170 kDa, with the most prominent band at60 kDa (lane 1). In the 27-month-old preparations...

Tài liệu Báo cáo khóa học: A multi-protein complex containing cold shock domain (Y-box) and polypyrimidine tract binding proteins forms on the vascular endothelial growth factor mRNA Potential role in mRNA stabilization pptx

... stabilization in normoxic conditions [62]. Interestingly, we observed that the insulin mRNA PTB binding site has an overlapping consen-sus CSD binding site. PTB, to date, has only been implicated in stability ... reporthere the identiﬁcation of complexes, binding to the VEGFmRNA 5¢ -and3 ¢-UTR, that contain cold shock domain(CSD) and polypyrimidine tract binding (PTB) RNAbinding proteins. Analysis of the CSD/PTB ... CSD/PTB binding sitesrevealed a potential role in VEGF mRNA stability, in bothnoninduced and induced conditions, demonstrating a gen-eral stabilizing function. Such a stabilizing mechanism hadnot...

Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

... permeabilized and stained for F-actin with rhodamine-conju-gated phalloidin. Th e eﬀects of toxin B and latrunculin B on the actin ﬁlaments wasexamined by preincubating the cells with either 10 ngÆmL)1toxin ... response to aniso-mycin, suggesting that an intact actin cytoskeleton is alsonecessary for anisomycin signaling. Surprisingly, treatmentof the cells with latrunculin B alone induced an increase ... p38 signaling minimally affects the transcriptionalcontrol of the CTGF/CCN2 gene.Next, we examined the CTGF/CCN2 mRNA turnoverby inhibiting new mRNA transcription with actinomycinD upon stimulation...

Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows speciﬁc detection of antigen in mammalian cells pdf

... seemsthat the scFvs fused to d1-EGFP are maintained in the cells by binding to antigen.Generation of soluble binding-defective and cysteine-deleted mutantsTo demonstrate that the observed intracellular ... redu-cing conditions existing in cell compartments, whichmay prevent the formation of intradomain disulﬁdebonds within the VH and VL domains resulting in lessstable molecules. Nevertheless, a number ... targets. In case intrabodies interfere with antigen function,they can mediate cell killing following binding(recently reviewed in [1]). Whilst a variety of cell typesincluding plant cells, fungal...

Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx

... beneﬁcial effects of glutamine; (a) bydetermining the relative uptake of glutamine; (b) bysearching for changes in the intestinal proteome; and (c) by examining glutamine incorporation into ... Research Institute Maastricht (NUTRIM), Department of Human Biology,Maastricht University, the NetherlandsGlutamine has an important function in the smallintestine with respect to maintaining the ... proteins, which are indicated in bold. NQ, No glutamine-containing peptides in spectrumpeaks. C-term, C-terminal part of protein; N- term, N- terminal part of protein.SpotAccessionnumber Protein...

Tài liệu Báo cáo khoa học: A possible role of mitochondria in the apoptotic-like programmed nuclear death of Tetrahymena thermophila Takashi Kobayashi and Hiroshi Endoh docx

... different in distribution and in num-ber from that of the green ﬂuorescent signals of the DePsipher dye seen in Fig. 1C and D, indicating thatthere is no interaction between DePsipher monomers and ... nuclearcondensation and oligonucleosomal DNA laddering[19]. The hypothesis that mitochondria are associated with nuclear condensation and ⁄ or DNA degradation in PND is linked with the notion of mitochondrialnuclease ... even in 1 mm of Mg2+(comparelane 2 and 3 in Fig. 4C), indicating a different naturefrom most other DNases. On the other hand, nicking activity was unaffected by Mg2+, as shown by the increased...

Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx

... by insulin via IRS-1). ILK combines with an adaptor protein Nck2 and a ﬁve LIM domain-containing protein named PINCH (a particularly interestingnew cysteine histidine protein) to form a ternary ... slightly.Cell adhesion to laminin was increased with the concen-trations of the coated laminin, and enhanced after insulintreatment at different concentrations of laminin. By con-trast, it was ... receptor binding protein2-son of sevenless protein/Ras/Raf/mitogen activatedprotein kinase kinase/mitogen activated protein kinasepathway [13].Another function of the PKB signalling pathway is the promotion...

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