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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition Troels ... amino acid sequence of a-helix F (hF) and the connecting loop to s3A (hF/s3A-loop) for the rate of latency transition. We grafted regions of the hF/s3A-loop fromantithrombin III and a1-protease ... complex,cleavage of the P1–P1¢ peptide bond, linkage of the activesite Ser of the protease to the carboxyl group of P1by anester bond and insertion of the N-terminal part of the RCLas strand 4...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... this work for the activation of ONC has nodetrimental effect on the conformational stability of the enzyme.Cytotoxic activity The effect of (Met1) removal on the cytotoxic properties of ONC was ... of the hydrolysis wasconfirmed by the disappearance of the (Met1)-ONC(M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The pH was adjusted to 2.5, 8.0 and 11.5 and the ... solvent of the matrix, and 0.5 lL of the mixturewas immediately applied to a sample probe spot.Cyclization reaction as a function of pH and temperatureIn order to determine the effect of pH and...
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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... Lopiano4 and Mauro Fasano11 Department of Structural and Functional Biology, and Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy2 Department of Biotechnology, University of Verona, ... impairment of the antioxidant defense of the cell, as in the case of DJ-1 or PTEN-induced putativekinase 1 mutations. Mitochondrial and proteasomedysfunction and oxidative stress could account for the selective ... intensity was significantly dif-ferent in at least one of the four groups considered(Fig. 2). Two groups of spots showing remarkablechanges in the isoform pattern in the four conditionswere easily...
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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... regardless of the type of substrate (acetyl amide or acetyl ester).Results and DiscussionAction of reversible effectors on AAA activity of BuChE The investigation of the effects of the ligands(tyramine, ... could reflect differences in the conformational plasticity of the active site gorge of these enzymes.Effects of benzalkonium on o-NPA and o-NAChydrolysis The hydrolysis of both o-NPA and o-NAC by ... (MayoClinic, Jacksonville, FL, USA) for the gift of ATMA and Dr S. Darvesh (Dalhousie University, Halifax,Canada) for the gift of o-NTFNAC. The authors aregrateful to Ellen Duysen and Hasmik Grigoryan(UNMC,...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... demonstrating that the J–I riseis not directly related to the reduction of the PQ pool.A further analysis of the influence of PQ reductionon FI was performed after the addition of dPQ to the thylakoid ... raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of stepI, and to retard the rise to F m. With dPQ, the J stepwas lowered and the subsequent ... on the relative amplitude and rate constant of the J–Iphase compared to the amplitudes and rate constants of the two other phases. The above should be gov-erned by the balance between the rate...
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Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

... concentration of the porphyrin was 0.62 mM), to investigate the effect of porphyrin b inding on the protein conformation. For thesespectra, a spectrum of the buffer containing the sameconcentration of ... structures of MCL.To obtain more quantitative information on the secon-dary structure of MCL and the effect of ligand binding on it, the far-UV CD spectra of MCL in the native state as well asin the ... structure of MCL, and effect of porphyrin bindingCD spectra of MCL recorded in the far-UV region and near-UV region are given in Fig. 7A and 7B, respectively.Spectra obtained in the presence of a...
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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

... ÔStains-AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.As the full-length Dp427 isoform of dystrophin does notenter the second dimension of conventional 2D gels, the expression level of ... viscosity of t he extract as a result of D NA, 2 lLofDNase I (200 units) was added per 100 lL of buffer [30].Following filtration through two layers of miracloth a nd the addition of four volumes of ... quantification of comparative immunoblotting data occasionally difficult.As differential centrifugation is b ased upon the d iffer-ences in the sedimentation rate of biological particles of different...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for Ala ... expression vector and 50 ng of FLAG-BMAL1 expression vector resulted inadequate levels of expression. The transcriptional activity of FLAG-BMAL1, and the suppressive activity of FLAG-DEC1, were ... ng of each). Transfection of 0.1 ng of the DEC1 expression vector did not significantlydecrease the transcriptional activity of CLOCK/BMAL1(Fig. 1B). Transfection o f 1 ng of the expression vectorresulted...
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Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... combination of a hierarchical up-regulation[16] of the las operon at low PK activity and a polareffect of the pyk deletion on expression of ldh.Animportant question is whether this affects the conclu-sions ... higherthan the flux control coefficients for the individual lasenzymes at comparable levels. From the data for PFKgiven in Andersen et al. [9], the flux control coefficienton the glycolytic flux of PFK activity ... the activity of the PTS system and therebyresult in further increases in hexose phosphate pools. The decreased LDH activity may contribute further tothis effect by causing an accumulation of...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "Contextual Analysis" doc

... extension of this comparison to other parts of speech and to punctuation, we can pre- dict the correct equivalent of и in 90% of its occurrences. Other examples of structural clarification of this ... governs the genitive case. The task of deciding whether or not to retain the &apos ;of& apos; falls upon the reader. The problem results, of course, from the syntactical com- pactness of inflected ... helpful, for instance, to have available, in coded form, the following infor- mation for words in a Russian sentence: part of speech of all words; case, number, and gender of nouns; the infinitive...
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