... mixed with an equal volume of SDS buffer(0.2 m Tris, pH 7.5, 0.3 m NaCl, 25 mm EDTA, 2% SDS)and twice extracted with phenol ⁄ chloroform, once extracted with chloroform, and precipitated with ... anti-flagWB anti-HAHA-ASF/SF2RNaseARNaseAw/o w/o with ++––+–+++++++–+–+–w/o w/o with ++––+–+++++++–+–+–w/o w/o with w/o w/o with WB anti-flagWB anti-HATIAR-flagBOIP-flagHA-hnRNP ... migrate into SGs in cells trea-ted with arsenite in combination with puromycin (datanot shown). Furthermore, ASF ⁄ SF2 co-localized exclu-sively with TIAR and not with Dcp1-positive Pbs [21],thereby...