Tài liệu Báo cáo khoa học: "Predicate Argument Structure Analysis using Transformation-based Learning" pdf

... containing semanticelements. This paper describes predi-cate argument structure analysis using transformation-based learning. An advan-tage of transformation-based learning isthe readability of learned ... Sweden, 11-16 July 2010.c2010 Association for Computational LinguisticsPredicate Argument Structure Analysis using Transformation-based LearningHirotoshi Taira Sanae Fujita Masaaki NagataNTT Communication ... to predicate argument structure analysis are statistical machine learning methodssuch as support vector machines (SVMs)(Pradhanet al., 2004). For predicate argument struc-ture analysis, we...

Tài liệu Báo cáo khoa học: Enzyme kinetics informatics: from instrument to browser pdf

... increasedconﬁdence in the data. A data analysis and submission tool, the kinetics-wizard, has been developed to allow the experimentalist to perform datacollection, analysis and submission to both ... notes and comments to the dataset.KINETICSWIZARD data analysis Following the data capture phase, the next step beforedata submission is data analysis [17], whereby kineticparameters are determined ... Ghaemmaghami S, Huh WK, Bower K, Howson RW,Belle A, Dephoure N, O’Shea EK & Weissman JS(2005) Global analysis of protein expression in yeast.Nature 425, 737–741.40 Malys N & McCarthy JEG (2006)...

Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc

... Cell numbers were counted using a Coulter counter (Multisizer 3, Beckman CoulterInc., Fullerton, CA, USA), using a 30 lm aperture.Experimental time-dependent regulation analysis K. van Eunen et ... workingon a quantitative analysis based on new metabolitemeasurements and a quantitative kinetic model [49].In conclusion, the quantitative approach of time-dependent regulation analysis applied in ... [45].In principle, regulation analysis allows us to dissectwhich part of the Vmaxregulation is caused by post-K. van Eunen et al. Experimental time-dependent regulation analysis FEBS Journal 276...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... using the program mosflm [38], and the ccp4 suiteof programs [39] was used for scaling. Structure deter-mination for hACMSD was performed by means ofthe molecular replacement technique, using ... A˚resolution were collected using the ID23-2 beamlineof the European Synchrotron Radiation Facility (Grenoble,France). An X-ray ﬂuorescence scan performed on the hA-CMSD crystals using the same beamline, ... investigated in the presence of 5 lm ACMSsubstrate.Crystallization and structure determinationCrystals of hACMSD were obtained using the vapour dif-fusion technique in hanging drop. A volume of...

Tài liệu Báo cáo khoa học: SREBPs: SREBP function in glia–neuron interactions pdf

... from the plasma to theCNS either in adults or during postnatal development[30]. Analysis of cholesterol synthesis using radioactivelabeling techniques has shown that almost all of thecholesterol ... 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGcR) and stearoyl-CoAdesaturase 2 (SCD2) [18].In summary, both expression analysis and molecularapproaches in vitro indicate a role for SREBP-2 in thecontrol of the cholesterol ... thesynthesis of MUFAs, PUFAs and cholesterol, which, after secre-tion, are bound by neuronal structures and affect neuriteoutgrowth, synaptogenesis and synaptic plasticity.SREBP function...

Tài liệu Báo cáo khoa học: Human Cdc45 is a proliferation-associated antigen pdf

... cytometry analysis of HeLa S3 cells after release from a double TdRblock. Asynchronously growing cells (log) served as a control for the classiﬁcation of cell-cycle phases. (B) Immunoblot analysis ... Immunoblot analysis of DNA polymerase d p125 and p50 subunits, PCNA and replication protein A p70 and p32 subunits inwhole-cell lysates of serum-starved T98G cells. (D) Immunoﬂuorescence analysis ... phase (S-phase fraction) and, on the otherhand, by detecting proliferation-associated proteins using immunohistochemistry. Proliferation markersare, in most cases, Ki-67 and PCNA [39]. Proliferationmarkers...

Tài liệu Báo cáo khoa học: Melatonin Nature’s most versatile biological signal? pdf

... restriction: meta -analysis. BMJ332, 385–393.170 Brzezinski A, Vangel MG, Wurtman RJ, Norrie G,Zhdanova I, Ben Shushan A & Ford I (2005) Effectsof exogenous melatonin on sleep: a meta -analysis. Sleep ... indicates that melatonin isuseful for ameliorating ‘jet-lag’ symptoms in air trave-lers (see the meta -analysis in the Cochrane database)[187].One of us examined the timely use of three factors(melatonin ... what would be expected in theabsence of any treatment [188]. More recently, a retro-spective analysis of the data obtained from 134 normalvolunteers ﬂying the Buenos Aires to Sydney trans-polar...

Tài liệu Báo cáo khoa học: Oral Presentations Integration of Metabolism and Survival pdf

... by using linkage analysis. Genomic DNA orcDNA of these patients were screened for the sequence variationsin the complete coding (43 exons) and flanking sequences of theFANCA gene by SSCP/HD analysis. ... correlated with survivalrates using Kaplan–Meier analysis and compared by means ofthe log-rank test. Significant risk factors were identified, and mul-tivariate Cox regression analysis was used to evaluate ... First, we have predicted 3-D structure ofcryptochrome using EsyPred, Robetta programs.Then, we haveshown that both cryptochrome and melanopsin may interact insilico using various computational...

Tài liệu Báo cáo khoa học: Solution NMR structure of an immunodominant epitope of myelin basic protein Conformational dependence on environment of an intrinsically unstructured protein doc

... wasused to convert the left structure to the right structure. The N-ter-minus is at the left for every structure. The best-ﬁt overlays of the10 lowest overall energy structures obtained with theARIA ... Ni+4< 3.5) did not improve the quality of the10 best structures, but reduced the occurrence ofNOE-violated structures over the ensemble of 100structures. Approximately 200 NOE distance res-traints ... spectra were processed using thexwinnmr package (Bruker BioSpin), and analyzed using sparky 3 (TD Goddard & DG Kneller, University ofCalifornia, San Francisco). Structure calculation and...

Tài liệu Báo cáo khoa học: Calcium-independent cytoskeleton disassembly induced by BAPTA pdf

... commercial sourceswere tested with similar results. The purity of D-BAPTAAM was routinely checked using MS. At least ﬁveindependent batches were tested i n t he course of this study.Nocodazole ... laser-scanning confocal microscopeevery 3 s for 10 min. Fluorescence intensities were quanti-ﬁed using Leica Confocal sofware.MicroinjectionCells grown on glass coverslips were injected u sing ... collected and stored at )80 °C. Cellextracts were used for quantitative d etermination of ATP, using an A TP determination kit (Molecular Probes). A ll ofthe reagents w ere prepared a ccording...

Xem thêm