Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf

... 4671 Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 Huma ... of histidine residues in PETN reductase inferred to be involved in ligand binding and counterpart residues in related enzymes and also the location of the tyrosine residueimplicated as proton donor in ... productive binding of steroid substrates: in the ﬁrst, a (small) sub population of enzyme bindsdirectly the steroid in the reactive conformation, with the remainder of the enzyme binding the unreactiveconformation....

Tài liệu Báo cáo khoa học: Electron transfer chain reaction of the extracellular ﬂavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium doc

... showed the pre-steady-state two-electron reduction of flavin, followedby interdomain one-electron transfer from flavin toheme, resulting in the formation of the flavin semi-quinone radical and reduced ... isosbesticpoint of heme (449.0 nm) decreased similarly in bothFig. 1. Stereo representation of the heme in CDH and the amino acids within 4 A˚ of the heme. Heme and Phe166 are indicated in color. The ... located in the cytochromedomain and approaches one of propionates of heme, was mutated to Tyr, and the thermodynamic and kinetic properties of the mutant (F166Y) werecompared with those of the...

Tài liệu Báo cáo khoa học: "Lexical transfer using a vector-space model" doc

... observing the environment including the context, world knowledge, and target words in the neighborhood. The most influential elements in the environment are of course the other words in the ... representations. II) The weight of each element for constraining the ambiguity of target words is determined automatically by following the term frequency and inverse document frequency in information ... sentence vector of an input sentence I and the sentence vector of an example sentence E from a bilingual corpus. We measure the similarity by computing the cosine of the angle between I and E. We...

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... and of the kanamycin insertion mutant nirF strain(SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom leftpanel and of SBN13 (DnirF) containingpEG276-nirF in the ... activity of NirFInterestingly, like NirN, NirF shares sequence similar-ity with the C-terminal d1heme-containing domain of cytochrome cd1. Strikingly, the axial ligand of iron in d1heme in ... in loss of in vivoNirF activity. We demonstrate here that the product of the nirF gene is aperiplasmic protein and, hence, must be involved in a late stage of the cofac-tor biosynthesis. In...

Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

... com-posed of an extracellular ligand-binding domain, atransmembrane domain and an intracellular tyrosinekinase domain. The binding of a ligand to the extracel-lular domain of the EGFR induces ... dimeriza-tion, activation of the intracellular kinase domain and autophosphorylation of tyrosine residues within the cytoplasmic domain of the receptor. The tyrosine-phosphorylated motifs of the EGFR recruit ... phos-phorylating the retinoblastoma (RB) protein. Activa-tion of cyclin–CDK complexes is counterbalanced byCDK inhibitors, including those of the kinase inhibitorproteins (KIP) family and the INK4...

Tài liệu Báo cáo khoa học: How disorder inﬂuences order and vice versa – mutual effects in fusion proteins containing an intrinsically disordered and a globular protein docx

... (2010) Solution structure of the C-terminalX domain of the measles virus phosphoprotein and interaction with the intrinsically disordered C-terminaldomain of the nucleoprotein. J Mol Recognit ... disordered C-terminaldomain of the measles virus nucleoprotein interacts with the C-terminal domain of the phosphoprotein via twodistinct sites and remains predominantly unfolded.Protein Sci 14, ... covalently linked. In view of obtaining further insight into the struc-ture of the fusion proteins, we estimated the content of a-helices, b-strands, b-turns and unordered regions by the cdsstr...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... decrease in PMP-BSA binding. It thusappears that tethering of the C-terminal end of the ectodomain is important for both IGF-II and PMP-BSA binding. In summary, the major ﬁndings obtained in the present ... binding must only be a localeffect because binding of PMP-BSA, which binds toother sites in the ectodomain of these heterodimers,resulted in the predicted amount of ligand binding.This loss of ... Seurin D, Segovia-Quinson B, Hardouin S& Binoux M (1986) Analysis of serum insulin-like growthfactor binding proteins using western blotting: use of the method for titration of the binding...

Tài liệu Báo cáo khoa học: Brain angiogenesis in developmental and pathological processes: regulation, molecular and cellular communication at the neurovascular interface pdf

... overexpression of SSeCKS reduced VEGFexpression and induced angiopoietin-1 (Ang-1), therebypromoting the expression of tight junction proteins and strengthening the bonds between brain endothelial cells[19]. ... and differences in the luminal–abluminal polarity of the endothelium. To bridge the gap between in vitro condi-tions and the actual environment, experiments shouldincorporate improved in vivo imaging techniques, ... to the actincytoskeleton via the binding of beta- and gamma-cate-nin to alpha-catenin [8]. Gap junctions have been iden-tiﬁed as BBB components [7]; however, their role in the function of the...

Tài liệu Báo cáo khoa học: Brain angiogenesis in developmental and pathological processes: therapeutic aspects of vascular endothelial growth factor doc

... organs,including the brain, is regulated by the VEGF system in combination with other signaling systems such as the angiopoietin–Tie, ephrin–Eph, Delta–Notch sys-tems, and the Wnt pathway.Furthermore, ... astrocytes, and the tight junctions of vascular endothelial cells (ECs) in the BBB are well organized with claudins and ZO-proteins through a decrease in angiogenesis signaling and an increase in the ... amongtissues in the body, and the risk of edema to brainfunctions should be carefully considered. Brain edemamay increase pressure in the cranial cavity and braintissue, resulting in suppression of...

Tài liệu Báo cáo khoa học: Brain angiogenesis in developmental and pathological processes: neurovascular injury and angiogenic recovery after stroke docx

... out-come and the number of circulating EPCs. In rodentmodels of focal cerebral ischemia, there was a strongcorrelation between the volume and severity of infarcts and the absolute number of circulating ... attractiveness of this approach lies in the factthat minocycline can be easily used in humans.Taken together, the accumulating experimental and clinical data suggest that MMPs (and perhaps otherextracellular ... ask whetherneurovascular responses after stroke can be reinter-preted in the context of angiogenesis in the brain. Is itpossible that some of the acute neurovascular events in the brain after...

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