Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... (2002) Crystal structure of afour-copper laccase complexed with an arylamine:insights into substrate recognition and correlation with kinetics. Biochemistry 41, 7325–7333.12 Piontek K, Antorini ... addi-tion, the crystal structure of TaLcc1 does not have a chloride attached tothe T2 copper, as observed in the crystal structure of MaL. The unique fea-ture of TaLcc1 and MaL as compared with other ... from the analogous structures of the ascomycete laccase (asco-laccase) MaL, thus giv-ing a comprehensive view of the structure and function of asco-laccases.Results and DiscussionOverall structure The...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... was transformed into BL21 (DE3) cells and grown at37 °C overnight in Luria–Bertani medium containing30 lgÆmL)1 of kanamycin. Flasks of 400 mL of Luria–Bertani medium (30 lgÆmL)1kanamycin) ... SJ, Brown LJ, Pank-hurst GJ, Luciani P, Aguilar MI, Mazzanti M, Berry-man MA, Breit SN et al. (2005) Crystal structure of thesoluble form of the redox-regulated chloride ion channelprotein ... auto-inhibitory regulation of NLS binding. J Biol Chem 275, 21218–21223.11 Kobe B (1999) Autoinhibition by an internal nuclearlocalization signal revealed by the crystal structure of mammalian importin alpha....

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... (Fig. 3A). Three of the ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, ... Suzuki5and Yasushi Kawata3,41 National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan2 Department of Food Science and Nutrition, Faculty of Human Life and Science, ... coordi-nated with six ligands: the ﬁve same ligands in theMn-reconstituted enzyme and an additional water oxy-gen, which, together with the OD2 of Asp165, theNE2 of His79 and the NE2 of His169,...

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... the structure analysis server stan [32]. This model has an Rwork of 0.180and Rfree of 0.206. As suggested by the electron density, theScatoms of all six cysteine side chains were modeled with ... L (2003) Crystal structures of human prostatic acid phosphatase in com-plex with a phosphate ion and a-benzylaminobenzyl-phosphonic acid update the mechanistic picture andoffer new insights ... the crystal structures of the ligand-free PhyK at 1.7 A˚resolution and of a cata-lytically incompetent PhyK mutant that suggest amodel for substrate binding. Comparison with thestructures of...

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... crystal structure of the complex of this protease with benzamidine.Results and DiscussionOverall structure The catalytic domain of DESC1 resembles an oblateellipsoid with diameters of 38 and ... showsexcellent stereochemistry, with 98.5% of all residues in themost favored and favored regions of the Ramachandranplot and r.m.s.d. values for bond and angle of 0.005 A˚and1.37 ° as shown in Table ... Lu DS, Futterer K, Korolev S, Zheng XL, Tan K,Waksman G & Sadler JE (1999) Crystal structure of enteropeptidase light chain complexed with an analog of the trypsinogen activation peptide....

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... hydrogen-bon-ded antiparallel b-strands (b6–b7), an a-helical hairpin(helices a5, a6) and a C-terminal strand (b8). Helix a5and strand b7 partially cover one side of the Ross-mann fold structure, thereby ... three-stranded b-sheets, each one consisting of the antiparallel b-strands b6, b7 of one monomer andstrand-b8 of the other. Solvent-accessible surface [15]calculations show that a substantial ... and has threehomologues in B. anthracis (str. A2012) (i.e.bant_01002171, bant_01004539 and bant_01004184) [4].All of them code for uncharacterized proteins thatshare sequence identities of...

Tài liệu Báo cáo khoa học: Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation docx

... Arg and Lys) within a dis-tance of 4 A˚. The meso- and psychrophilic structureshave the same number of salt-bridges and only twofewer than the thermophilic structure (Table 3). An important ... domain of Vibrio proteinase encompasses amino acids 140–420 of the 530 amino acid prepro-enzyme [34]. The modelwas reﬁned at a resolution of 1.84 A˚ with an R-factor of 14.1% and an Rfreevalue of ... of six a helices, one3 ⁄ 10 helix, a b sheet made of seven parallel strandsand two b sheets made of two antiparallel strands(Fig. 1B). Determination of the structure conﬁrms thepresence of...

Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx

... thebehaviourofbothproteinswithregardtothesesubstrateanalogues. Inhibition and site-directed mutagenesis of T. bruceigGAPDHIn the absence of a 3D structure of a complex of T. bruceigGAPDH with an analogue ... ﬁrst crystal structure of a stable isosteric analogue of 1,3-bisphospho-D-glyceric acid (1,3-BPGA)bound to the catalytic domain of Trypanosoma cruzi glycosomal glyceraldehyde-3-phosphate dehydrogenase (gGAPDH) ... F.S., Van Voorhis,W.C., Kuntz, I.D., Hol, W.G.J. & Gelb, M.H. (2001) Adenosineanalogues as selective inhibitors of glyceraldehyde-3-phosphate dehydrogenase of Trypanosomatidae via structure- based...

Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc

... biosynthesis of the plant hormone indole-3-acetic acid Anja Schu¨tz1, Tatyana Sandalova2, Stefano Ricagno2, Gerhard Hu¨bner1, Stephan Ko¨ nig1and Gunter Schneider21Institute of Biochemistry, ... fromL-trypto-phan, and consists of three steps: (a) the conversion of tryptophan to indole-3-pyruvic acid; (b) the formation of indole-3-acetaldehyde; and (c) the production of IAA(Fig. 1). The ﬁrst step of ... secondary structure elements of IPDC are shown inFig. 4, together with the aligned amino acid sequences of IPDC and ZmPDC. The topology of the IPDC monomer issimilar to that of ScPDC and ZmPDC with...

Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... determined the structure of HtA by NMR methods. The structure consists of one a-helix, a helical turn and seven b-strands thatform an N-terminal hairpin and an anti-parallel b-sheet, with a characteris-tic ... 2385involvement of a catalytic pair constituted by an acid and a base on each side of the hydrolyzed bond [16].E96 and H137 in a-sarcin and E58 and H92 in RNaseT1 act as the base and acid groups, ... agrees with the arrangement present inother ribotoxins, and is fully compatible with thedistance restraints discussed below.After seven cycles of NOE assignment and structure calculation by cyana⁄...

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