Tài liệu Báo cáo khoa học: The Alzheimer b-peptide shows tem

Tài liệu Báo cáo khoa học: The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 31-helix, b-strand and random coil secondary structures doc

... 3949 The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 3 1 -helix, b-strand and random coil secondary structures Jens Danielsson, Ju ¨ ri Jarvet, Peter Damberg and ... residue. The bond lengths used were 1.33, 1.45 and 1.52 A ˚ and the ﬁxed angles between the bonds were: 58°,64° and 75°. The rotations around the...

Ngày tải lên : 20/02/2014, 01:20

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Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identiﬁcation of new components and synergies between complexes ppt

... enhancement was attributed to the physical prox- imity of the enzymes in the mini-cellulosomes and to cellulose targeting of the complexes via the CBM of the mini-scaffoldin [21]. The most active mini-cellulo- some ... con- trol sample and all the fractions, except for F1, which was found to be approximately ﬁvefold more active than the others. The cellulosomes present...

Ngày tải lên : 18/02/2014, 08:20

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Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt

... a-helical coiled -coil structures are known to mediate homo- and hetero-dimerization of proteins, and these sequences may facilitate the speciﬁc interactions between BAP31 and myosin heavy chain. Furthermore, ... in the BAP31 immunopre- cipitate, indicate that the association between BAP31 and c-actin was the result of speciﬁc interactions. Discussion We describe in thi...

Ngày tải lên : 20/02/2014, 23:20

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Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... pH (6.0–8.0) and at low temperatures (0–35 °C). We focus on the function and relationship of the two single domains. Additionally, the possibility that fusion of the PhyH N-terminal domain to other single-domain ... (Fig. S1). There are two pairs of cysteine residues, Cys142 and Cys193 in PhyH-DII and Cys445 and Cys495 in PhyH-DI. These residues are at the same positions a...

Ngày tải lên : 14/02/2014, 15:20

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Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... indicated time points. The mixes were then incubated with [c- 32 P]-ATP and MBP (10 l g) for 30 min. The top panel shows the kinase assay visualized by autoradiography and the bottom panel shows the Coo- massie ... and (B) the top panel shows the kinase assay visualized by autoradiography and the bottom panel shows the Coomassie Brilliant Blue-stained SDS ⁄ P...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... in the ChIP assays [60]. Similarly, ChIP experiments on the cyclin A, Cdc2 and cyclin B2 genes suggest that E2F4 and E2F6 bind to the promoters in G 0 and to some extent also in G 1 [55]. Furthermore, ... that the exact binding location is not identiﬁed in the experiments. Therefore, it is not clear whether the CDE ⁄ CHR is involved in binding these factors. Consistent wit...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

... Maintaining the balance between cell survival and apoptosis is critical in the maintenance of a healthy organism, and tipping the equilibrium in one or another direction results in either Keywords apoptosis; ... reagents and the recombinant human TRAIL ⁄ APO 2 ligand were purchased from Invitrogen and Feldan Bio (St-Laurent, QC, Canada), respectively. The caspase 3 substra...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf

... of the ARE I-AUUUA and ARE III-AUUUA sequences used primers SJS172 and SJS173, and SJS174 and SJS175, respectively. Mutation of the atypical AU-rich sequence used primers SJS259 and SJS260, and ... SJS260, and the creation of the ARE II ⁄ ARE IV double mutant used primers SJS261 and SJS262. The mutagenesis of ARE I and III introduced HindIII restriction sites and...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... isolated and analyzed by northern blot using Fluc and Rluc riboprobes. The radioactive signals were quantiﬁed by the STORM 820 equipment and IMAGEQUANT software (Molecular Dynamics). The Rluc ... [15]. On the other hand, other studies indicated that ASF ⁄ SF2 activates the translation of mRNAs bearing ASF ⁄ SF2 binding sites within the coding region in intact cells [16,54]....

Ngày tải lên : 16/02/2014, 15:20

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Tài liệu Báo cáo khoa học: The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium docx

... with thiol groups, and the cadmium speciation of the wild-type and the CeMT-2 knockout strain was distinctly different to the CeMT-1 and double knock- outs. Taken together, and supported by a simple ... bear the hallmarks of metallothioneins, i.e. they are small and cysteine-rich, and their expression is induced by metals [24]. More recently, RNA interference (RNAi) and...

Ngày tải lên : 16/02/2014, 15:20

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