Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

... dimerization of cystatin F could be a processregulating its inhibitory properties. Screening of proteases for their inhibition showedthat cystatin F is different from other cystatins, both in terms of ... weight stand-ards. (B) Inhibitory activity of cystatin F against papain. Cystatin F (500 nM) was incubated 15 min at 37 °C in phosphate bufferpH 6.0 with different concentrations of dithiotreitol. ... aim of our study was to identify potential tar-gets of cystatin F among endogenous lysosomal cys-teine proteases. First we found that dimers of cystatin F are inactive as inhibitors of cysteine...

Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

... thank Prof. Steve C.Fry, Institute of Cell and Molecular Biology, Univer-sity of Edinburgh, Edinburgh, UK, and Dr AlbertSchulte, Ruhr University of Bochum, Germany, forcritical reading of the ... chitooligosaccharides, and chitin. The ini-tial velocity of the enzyme for concentrations of thesubstrates ranging from 0 to 2.0 mm was determinedafter 5 min of reaction. Given the fact that chitinase Aproduced ... endo property of chitinase A is further veriﬁedby the formation of reaction intermediates of varyinglength (Fig. 2).The role of two catalytic amino acid residues(Glu315 and Asp392) in the enzyme...

Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... Schematicrepresentation of the different Mxi1 isoforms tested for suppres-sive potential in the REF transformation assay. All of the isoformscarried Myc tags on their COOH termini. SID, Sin3 interactingdomain; ... absence of doxycycline. (B) Silver staining of an anti-Flag IP per-formed on lysates from HeLa Tet ON cells expressing either empty vector, FLAG-Mxi1-SRa or FLAG-Mxi1-SRb after 24 h of induction ... of foci forma-tion, with the level of foci formation obtained for the empty vectorcontrol point set to 100%. In the SRblow point, one-ﬁfth the usualamount of SRb expression construct was introduced....

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... v) fetal bovine serum (Biochrom KG, Berlin, Ger-many). Paired cultures of cells growing in normal media or in iron-rich media were set up and maintained for differentperiods of time as indicated. ... the other hand, a recent in vitro study examining the effect of iron loading ongene expression in HepG2 cells by differential displayrevealed that iron can affect mRNA levels of proteinsunrelated ... Forphosphatidylserine determination, cells were washed withbinding buffer and incubated with Annexin V-FITC for15 min at room temperature. Cells were deposited immedi-ately in poly(l-lysine)19coated...

Tài liệu Báo cáo khoa học: Solution properties of full-length integrin aIIbb3 reﬁned models suggest environment-dependent induction of alternative bent ⁄extended resting states doc

... available:Fig. S1. Alignment of the long loop region in the calf-2 module of integrin’s a subunits.Fig. S2. A gallery of structures predicted by Robetta[20] for the long loop in the calf-2 region of ... shown in Fig. 1.Apart from studies demonstrating the necessity of thiscleavage for the proper function of avb5[47], little isknown of its role in integrins lacking an a-I ⁄ A domain(see ... bTD module at the main chain of D606 to dock itagainst the calf-2 domain with its C-terminus in prox-imity of the N-terminus of the b3‘open’ TM helix.Overall evaluation of the modelsThe ﬁve...

Tài liệu Báo cáo khoa học: Electrical properties of plasma membrane modulate subcellular distribution of K-Ras ppt

... Membrane binding properties of K-Ras. (A) Membrane fractions of CHO-K1 cells expressing YFP-K-RasC14or YFP-K-Rasfullor CFP-H-RasC20were obtained as described in Fig. 1B and then incubated for ... and cytosolicfractions from nontransfected cells or cells expressing YFP, YFP-K-RasC14or YFP-K-Rasfullwere obtained as described in Fig. 1. Membranefractions of transfected cells were incubated ... per-formed Triton X-114 partitioning assay on particulatefractions of cells transiently expressing the fusionproteins [34,35] (Fig. 1C). Fifty percent and 44% of YFP-K-RasC14 and YFP-K-Rasfull,...

Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

... aminoacid residues of the heparin-binding site (HBS) of VVA2 (166–194). (B) Inhibitory effects of VVA1 on the binding of VVA2 to liposomes. Afterincubation of VVA1, VVA2 or the mixture of VVA2 ... to bind to themembrane of cells. Inhibitory effects of VVA1 on the hemolytic and cytotoxic activity of VVA2The effects of VVA1 on the hemolytic activity of VVA2 were studied by incubation of ... N-terminal and theC-terminal pair of a-helices of VVA1 can bind toVVA2 independently of each other and thus enable thedirect binding by one molecule of VVA1 of two mole-cules of VVA2. This further...

Tài liệu Báo cáo khoa học: Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner pptx

... lanes2, 4 and 6 in Fig. 1A but the use of a higher exposure allowsthe effects of the mutants to be more evident. Again shiftedMyb-bands were observed in addition to the main 75 kDaband (Fig. 1B, ... expressing lamin (pLam) and full-length human c-Myb(pDBT-hcM-FL) were transformed into the a-mating type of the samestrain. Mating was performed to create the diploid combinationsindicated in ... one of a few major Myb-interacting proteins. Themoderate basal level of sumoylation was greatly enhancedby cotransfection of PIASy, an E3 ligase for SUMO-1.The functional consequence of abolishing...

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... shown).Role of PKA in the mitochondrial targeting of CYP2E1The role of PKA in mitochondrial targeting of CYP2E1 was investigated using two approaches. Theﬁrst approach involved measuring the level of ... mitochondrial ferredoxin (FDX) + ferredoxinreductase (FDXR), homologs of mammalian ferredoxin and ferrodoxin reductase [27,28].ResultsExpression of intact and truncated rat CYPs in Saccharomyces ... determine the preciseintramitochondrial localization of CYP2E1 in trans-formed yeast cells. In the ﬁrst approach, we assessed theeffects of treatment of mitochondria and mitoplastswith trypsin....

Tài liệu Báo cáo khoa học: Down-regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism docx

... of folic acid, i.e. the smallintestine.Expression of the RFC protein in BBM of theintestineThe effect of chronic alcoholism on the level of expres-sion of the RFC protein at the BBM surface ... sec/mg protein)Fig. 2. [3H]Folic acid transport in intestinal BBMVs at differentintervals during the course of ethanol administration. An incubationbuffer of pH 5.5 and a [3H]folic acid ... and mid-villus could be seenduring immunohistochemical staining. Thus, chronicethanol feeding imparts its effect more strongly in pro-liferating and differentiating cells in the context of RFC...

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