... Institute of Advanced IndustrialScience and Technology (AIST) and JapanBiological Informatics Consortium (JBIC),Tokyo, JapanDatabaseDNA data bank of Japan accessionnumbers: olvas, AB063484; nanos3,AB306931; ... diffusion constants of 11.7, 12.9 and5.4 lm2Æs)1, respectively, in the part of the cytoplasmoutside of the granules. FRAP analysis in the gran-ules provided typical recovery curves of these ... FCS and FRAP analyses of Olvas–GFP, Nanos–GFP andTudor–GFP in HeLa cells. Diffusion of Olvas–GFP, Nanos–GFP andTudor–GFP was measured by FCS in the cytosol outside the region of the granule....