... 1B shows the patternseen after the development of the blot. With increasein time of incubation, the intensity of the band for the monomeric protein decreased, whereas the bands for the higher ... Figure 4A shows the kinetics of aggregation of a-synuclein in the presence of 100 lm MPTP and the effect of 50 lm dopamine on the aggregation process. Dopamine delayed the lagphase of aggregation ... are of a-synuclein incubated alone (A), in the presence of 100 lM MPTP (B), in the presence of 200 lM MPTP (C), in the presence of 100 lM MPP+(D), in the presence of 200 lM MPP+(E), in the...