Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... pGL3e:Prm 3a AP)1*,pGL3b:Prm3abAP)1*, pGL3e: Prm3abAP)1*, pGL3b:Prm3aaAP)1*, pGL3e:Prm3aaAP)1*, pGL3b:Prm3aaaAP)1* and pGL3e:Prm3aaaAP)1*.Mutation of the Oct-1 element with the ... sequence aaATGCa to aaTTCCa (core bases shown in uppercase letters)centered at )1 23 within Prm3 was performed using the mutator primers Kin175 (5¢-CACCAGAGCTACTTACACTGAATTCCAGAATAATCACAAGCAAATC -3 ; ... )94).(7) Prm3aab; pGL3b:Prm3aab & pGL3e:Prm3aab. (PrimerKin160; 5¢-dGAGAGGTACCGCAAATCTTCTCTCGCCTCC -3 , corresponding to NTs )106 to )86).(8) Prm3aaa; pGL3b:Prm3aaa & pGL3e:Prm3aaa. (PrimerKin161,...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... from human adenocarcinomasAnna Maria Luciani1, Sveva Grande1, Alessandra Palma1, Antonella Rosi1, Claudio Giovannini2,Orazio Sapora 3 , Vincenza Viti1 and Laura Guidoni11 Dipartimento ... irradiated: black) and A ⁄ (Lys + Ala) (controls: dotted white;irradiated: dotted black) as obtained from 1D and 2D COSY spectra of HeLa and MCF-7cell samples. Data are the mean ± SDvalues of ... absent(Fig. 1A, B). The same considerations hold for the peaks at 1.22 and 1.40 p.p.m. (Table 1 and Figs 2 and 3) . These signals most likely arise from the aggregation of large molecules because...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... (5¢-GCATAGCGATGTGGACGA -3 ) and QaCgClp2 (5¢-GAGGACCGAGACCGTGAA -3 ). The abbreviations ‘Qs’ and ‘Qa’ refer, respectively, to sense and antisense primers. Accurate ampliﬁcation of the targetamplicon was checked by obtaining ... and is characterizedby the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues toproduce adult tissues (i.e. the development of the gills and ... sequencingchemistry.Real-time quantitative PCRQuantitative RT-PCR analysis was performed using the iCycler apparatus (Bio-Rad, Hercules, CA, USA). TotalRNA was isolated from oocytes, embryos, larvae and adulttissues...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... Delineation of the molecular basis for our observations is essential toevaluate the elevated DNase I activity in the sera of patients with AMI and to validate the use of serumDNase I activity as ... cells and a primer set of the forward pri-mer DN-5R and the reverse primer DN+854X (sequences5¢-CGGAATTCTCAGGATGAGGGGCATGAAG -3 and 5¢-CGCTCGAGGCTGCTCACTTCAGCATCAC -3 , res-pectively) and directionally ... lucif-erase activity of the ) 138 6M construct was at leasteightfold higher than that of pGL3–basic vector and was half that of pGL3 promoter vector. These ﬁndingsdemonstrate the promoter activity of the...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... Characterization of the interaction between the plasmamembrane H+-ATPase of Arabidopsis thaliana and a novelinteractor (PPI1)Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida ... primers: gatggatcccatATGGGTGTTGAAGTTGTA annealing around the start codon of the Ppi1 ORF and gactcgagATTAGTCGACTTCTTACGCannealing just before the putative transmembrane domain(capital letter in the ... Michelis and Spanswick [33 ]. The PM H+-ATPase activity was evaluated as the difference between total activ-ity and that measured in the presence of 100 lm vanadate(less than 10% of total activity...

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... 5¢-GACACCGTAGGTTGAGCCGCCAATCGTCCC -3 ; NP5, 5¢-CTTTAACTTGTTGGGCACTGGCATTG -3 ; NP6, 5¢-TTGATCGATTCTGTCTATGCCCCA -3 along with the adaptor primers: AP1 (5¢-GTAATACGACTCACTATAGGGC -3 ) and AP2 ... endo- and exo- peptidases are widespread innature and found in viruses, archaea, bacteria and euk-aryotes. The biological importance of peptidases are clearly indicated by the fact that 2% of all ... PCR was performed in 50 lL containing 1 ng of genomic DNA as template, 0.2 mm dATP, dCTP, dGTP and dTTP, 0.2 lm of upstream primer (OP17: 5¢-GAAAAACCATGGTGAATGAATACCAAGCGACT -3 ) and downstream...

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... 5¢-TATGGATCCTTAATTAGAAACAAACTGTCTGATAAACP2OH 5¢-CTCGAGATTAGAAACAAACTGTCTGATAAACCP1Z 5¢-ATACCATGGCTGGAGAAGACTTTAAGATCP1ZH 5¢-CATATGACTGGAGACTTTAAGATCP2Z 5¢-TATGGATCCTCACCACCCAATTTCGGAAAGP2ZH ... SymbolsO, Z and H stand for Sno1p, Snz1p and His-tag, respectively.Underlined are the restriction enzyme sites.Primer SequenceP1O 5¢-ATACCATGGACAAAACCCACAGTACAATGP1OH 5¢-CATATGCACAAAACCCACAGTACP2O ... mMAPAD and 7 units of glutamate dehydrogenase at 37 °C for 90 min. After centrifugation for 1 min, the absorbance of the supernatant was read at 36 3 nm for APADH. The curve drawn is a theoretical...

Tài liệu Báo cáo khóa học: Non-speciﬁc depolymerization of chitosan by pronase and characterization of the resultant products pptx

... depolymerization of chitosan by pronase and characterization of the resultant productsAcharya B. Vishu Kumar1, Lalitha R. Gowda2 and Rudrapatnam N. Tharanathan11Department of Biochemistry and ... 15 13 1518.41. Watanabe,T.,Kobori,K.,Kiyotaka,Miyashita,Fujii,T.,Sakai,H. & Makot, Uchida and Tanaka, H. (19 93) Identiﬁcation of glutamic acid 204 and aspartic acid 200 in chitinase A1 of ... internal reference for comparison of band absorbance. Absence of a sharp and convolutedspectral band around 36 00 30 00 cm)1in the spectra of bothchitosan and LMWC indicated the absence of free...

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... Àðk A =k0Þ= A ð1Þwhere k A and k0 are the inactivation rate constantseither in the presence or in the absence of variousconcentrations of a ligand A, k2 and k1 are the rate constants of ... legitimate to apply the Scrutton and Utter equation [40] to the inhibition data.Data analysesData were analysed by means of sigmaplot 2001 (SPSSScience Software Gmbh, Erkrath, Germany). Data for ... 6 73 751. 3 Hrazdina G & Jensen RA (1992) Spatial organization of enzymes in plant metabolic pathways. Annu RevPlant Physiol Plant Mol Biol 43, 241–267.4 Fujiwara H, Tanaka Y, Yonekura-Sakakibara...

Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc

... the parallel b-helixFig. 1. Schematic organization of the pectinase gene cluster in T. maritima (TM0 433 -04 43) . pelA and pelB are shown as grey arrows. Adja-cent genes are a- glucuronidase (agu), ... Erwinia carotovora (CAA35998.1), PehX Erwinia chrysanthemi (AAA24842.1), Ralstonia solanacearum K60PehC (AAL24 033 .1), PG Thermoanaerobacterium thermosulfurigenes (AAB08040.1), Pgx Aspergillus ... tubingensis (CAA68128.1), Pgx2 Arabi-dopsis thaliana (AAF21195.1). The mode of action (endo or exo) and the amount of GalpA cleaved off, respectively, are annotated in paren-theses. A question mark indicates...

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