... (a-mg) were
used as substrates, the enzyme activity was determined as
the rate of hydrolysis of the substrate by measuring the
release of glucose according to the enzymatic method of
NADP
+
reduction ... locates on the long loop region
followed by Nb4, and the side chain of Val200 faces
toward the inside of the c atalytic cleft [39]. Figure 4 shows
the h ypot...
... therefore ampli-
fied the open reading frame from the genomic DNA.
We called the genes gld1 and gld2, respectively.
Comparison of the cDNA of gld1 with the genome
sequence revealed that the genomic DNA ... affinities for the two substrates and sim-
ilar maximal rates. The activity in the oxidizing reaction with glycerol as
substrate was under our detection limit. Alt...
... contribute to the patho-
genesis of CRC include the accumulation of mutations
and the deregulation of gene expression. Of particular
interest is the fact that a significant number of the pro-
teins ... examined the expression status of several of the
identified proteins using western blotting. These repre-
sentative proteins were selected based on changes of
more t...
... than, that of authentic ubiquinone-10, as the mix-
ture of both authentic ubiquinone-10 and extracts from
LN902(Dcoq7) yielded two separable peaks (Fig. 3A).
The identification of the main quinone-like ... contributions of
multiple types of quinones should be considered when
attempting to account for the long life span of the
C. elegans clk-1 mutant. However, because o...
... 4673–4680.
J. To
¨
zse
´
r et al. Comparison of two potyvirus proteases
FEBS Journal 272 (2005) 514–523 ª 2004 FEBS 523
Comparison of the substrate specificity of two potyvirus
proteases
Jo
´
zsef To
¨
zse
´
r
1
, ... modeling, the Arg side chain of the substrate
may partially compensate for the loss of the Lys side
Table 1. Comparison of the sp...
... that
H. thermophilus POR functions toward pyruvate syn-
thesis in vivo.
To investigate the reaction mechanism of H. thermo-
philus POR, we measured the EPR spectra of the
enzyme in the presence of ... activity was defined as the reduction of 2 lmolÆ-
min
)1
of metronidazole (corresponding to the decarboxyl-
ation of 1 lmolÆmin
)1
of pyruvate on the assumption that...
... angles occupy allowed regions of the Rama-
chandran map [32,33]. Comparison of the monomer of
the substrate- free form of E. coli PS [13] with the
monomers of nPS in the pantoate-bound form at full
occupancy ... of pantoate. The electron density for
the two molecules of pantoate bound at the active site
of nPS can be unambiguously distinguished in Fig. 4B....
... However, neither the
specificity of bacitracin for PDI nor the detailed mech-
anisms of inhibition of PDI have been investigated.
Furthermore, since the original reporting of the inhibi-
tion of PDI ... neither showed any change in activity upon
the addition of bacitracin (P > 0.5).
For further study of the potential mechanism of
action, the reaction conditio...
... bc
1
sub-complex, but the presence
of only one of these two subunits does not substitute
for the other. After the addition of ISP, the binding of
the last subunit (i.e. Qcr10p) finally occurs. These find-
ings ... [12,29,32,41]. Of course, the
possibility that other protein components of the respi-
ratory chain, such as subunits of the cytochrome c
oxidase, or o...
... the
lack of domain B protruding out of the TIM barrel in
the place of loop 3 connecting the b3-strand to the
a3-helix [9,21]. Sharing domain B by rBATs and
GH13 enzymes, and especially the sequence of ... [3,11–13]. Of the three GH
families of the clan GH-H, it was the family GH13
that was originally established as the a-amylase family
[14–17]. At present it be...