Tài liệu Báo cáo khoa học: Comparison of the substrate specificity of two potyvirus proteases doc

Báo cáo khoa học: Val216 decides the substrate specificity of a-glucosidase in Saccharomyces cerevisiae doc

Báo cáo khoa học: Val216 decides the substrate specificity of a-glucosidase in Saccharomyces cerevisiae doc

... (a-mg) were used as substrates, the enzyme activity was determined as the rate of hydrolysis of the substrate by measuring the release of glucose according to the enzymatic method of NADP + reduction ... locates on the long loop region followed by Nb4, and the side chain of Val200 faces toward the inside of the c atalytic cleft [39]. Figure 4 shows the h ypot...
Ngày tải lên : 07/03/2014, 16:20
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Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc

Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc

... therefore ampli- fied the open reading frame from the genomic DNA. We called the genes gld1 and gld2, respectively. Comparison of the cDNA of gld1 with the genome sequence revealed that the genomic DNA ... affinities for the two substrates and sim- ilar maximal rates. The activity in the oxidizing reaction with glycerol as substrate was under our detection limit. Alt...
Ngày tải lên : 19/02/2014, 06:20
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Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx

Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx

... contribute to the patho- genesis of CRC include the accumulation of mutations and the deregulation of gene expression. Of particular interest is the fact that a significant number of the pro- teins ... examined the expression status of several of the identified proteins using western blotting. These repre- sentative proteins were selected based on changes of more t...
Ngày tải lên : 16/02/2014, 15:20
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Tài liệu Báo cáo khoa học: Comparison of a coq7 deletion mutant with other respiration-defective mutants in fission yeast doc

Tài liệu Báo cáo khoa học: Comparison of a coq7 deletion mutant with other respiration-defective mutants in fission yeast doc

... than, that of authentic ubiquinone-10, as the mix- ture of both authentic ubiquinone-10 and extracts from LN902(Dcoq7) yielded two separable peaks (Fig. 3A). The identification of the main quinone-like ... contributions of multiple types of quinones should be considered when attempting to account for the long life span of the C. elegans clk-1 mutant. However, because o...
Ngày tải lên : 18/02/2014, 14:20
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Tài liệu Báo cáo khoa học: Comparison of the substrate specificity of two potyvirus proteases doc

Tài liệu Báo cáo khoa học: Comparison of the substrate specificity of two potyvirus proteases doc

... 4673–4680. J. To ¨ zse ´ r et al. Comparison of two potyvirus proteases FEBS Journal 272 (2005) 514–523 ª 2004 FEBS 523 Comparison of the substrate specificity of two potyvirus proteases Jo ´ zsef To ¨ zse ´ r 1 , ... modeling, the Arg side chain of the substrate may partially compensate for the loss of the Lys side Table 1. Comparison of the sp...
Ngày tải lên : 19/02/2014, 16:20
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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

... that H. thermophilus POR functions toward pyruvate syn- thesis in vivo. To investigate the reaction mechanism of H. thermo- philus POR, we measured the EPR spectra of the enzyme in the presence of ... activity was defined as the reduction of 2 lmolÆ- min )1 of metronidazole (corresponding to the decarboxyl- ation of 1 lmolÆmin )1 of pyruvate on the assumption that...
Ngày tải lên : 16/02/2014, 09:20
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... angles occupy allowed regions of the Rama- chandran map [32,33]. Comparison of the monomer of the substrate- free form of E. coli PS [13] with the monomers of nPS in the pantoate-bound form at full occupancy ... of pantoate. The electron density for the two molecules of pantoate bound at the active site of nPS can be unambiguously distinguished in Fig. 4B....
Ngày tải lên : 16/02/2014, 09:20
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Tài liệu Báo cáo khoa học: Bacitracin is not a specific inhibitor of protein disulfide isomerase pptx

Tài liệu Báo cáo khoa học: Bacitracin is not a specific inhibitor of protein disulfide isomerase pptx

... However, neither the specificity of bacitracin for PDI nor the detailed mech- anisms of inhibition of PDI have been investigated. Furthermore, since the original reporting of the inhibi- tion of PDI ... neither showed any change in activity upon the addition of bacitracin (P > 0.5). For further study of the potential mechanism of action, the reaction conditio...
Ngày tải lên : 16/02/2014, 14:20
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Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf

Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf

... bc 1 sub-complex, but the presence of only one of these two subunits does not substitute for the other. After the addition of ISP, the binding of the last subunit (i.e. Qcr10p) finally occurs. These find- ings ... [12,29,32,41]. Of course, the possibility that other protein components of the respi- ratory chain, such as subunits of the cytochrome c oxidase, or o...
Ngày tải lên : 18/02/2014, 08:20
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Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt

Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt

... the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to the a3-helix [9,21]. Sharing domain B by rBATs and GH13 enzymes, and especially the sequence of ... [3,11–13]. Of the three GH families of the clan GH-H, it was the family GH13 that was originally established as the a-amylase family [14–17]. At present it be...
Ngày tải lên : 18/02/2014, 13:20
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