Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx

... (2005) 655–672 ª 2005 FEBS Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells Seiko Masuda1, Makoto ... distributed in various cells, such as synovial lining and sublining cells and vascular cells, as well as in extracellular matrix ﬁbers [46]. In ourstudy, synovial membranes from a patient with inac-tive ... moderate staining in the interstitium (purple arrows). (C–E) Staining of sPLA2-V in three active RA tissues. In all cases, intense staining of the granulation tissue in the sublining interstitium...

Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane afﬁnity and hyper-anticoagulant activity in mouse plasma pptx

... the kinetic bind-ing data describing the sum of these kinetic eventscould not be adequately simulated by simple bindingmodels commonly used in the kinetic ﬁtting of pro-tein–protein interaction ... containing over 20 grouped PS molecules).We have shown that doubling the PS concentration in the membrane results in an increase in the number ofbinding sites (compare Fig. 3D and 3E) and also in ... silver staining. Protein C vari-ants and molecular weight markers (MWM) ran in each lane are indicated. The location of heavy chain (HC), light chain (LC) and thrombin (IIa) is also indicated.Anticoagulant...

Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx

... signalling proteins, and to theexpression of pro- and in ammatory cytokines. Thereis evidence that the heteromeric guanine nucleotide-binding regulatory protein (G protein) is also involved in ... change. Ligands include hormones,biogenic amines, histamine, serotonin and lipid deri-vates, but also immunological and in ammatory medi-ators such as chemokines. Heterotrimeric G proteins are localized ... activation of JNK.Other MAP kinase inhibitors as well as G-proteininhibitors should be investigated to ﬁnd out the partici-pating proteins in RGS1 and RGS2 modulation. In conclusion, our results...

Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx

... the cells with BEL induced a signiﬁcant accu-mulation of cells in G2⁄ M and S, and a concomitantdecrease of cells in G1, with respect to untreated cells. In contrast, pyrrophenone induced ... phospholipase A2 in fatty acid incorporation, phospholipid remodeling,lysophosphatidylcholine generation, and secretagogue-induced arachidonic acid release in pancreatic islets and insulinoma cells. ... signaling.We also show that U937 cell iPLA2-VIA activity isregulated in a cell-cycle-dependent manner, with max-imal activity at G2⁄ M, steadily declining during G1, and increasing again in...

Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc

... validated in vitro andusedtoexaminethe branch-point kinetics in detail and to obtain insights intothe kinetic controls of methionine and threonine synthesis in plants.The branch-point between ... methionine and threonine biosynthesis pathways in Arabidopsis thefollowing procedure was used.Firstly, the kinetic links inside the branch-point and between the branch-point system and the ... to cysteinevia a ping-pong mechanism. Reactions are irreversible and inhibited by inorganic phosphate. The modelling procedureincluded an examination of the kinetic links, the determin-ation...

Tài liệu Báo cáo khoa học: Degradation of tropoelastin by matrix metalloproteinases – cleavage site speciﬁcities and release of matrikines pptx

... collagens,gelatin, and laminin, and also non-ECM proteins,including pro-tumor necrosis factor-a and a2-macro-globulin [2]. MMP-9 (gelatinase B) is secreted by neu-trophils and macrophages and has ... 150 mm; Dionex) and elution using a linear binarygradient: 5–65% ACN in 0.1% formic acid in 35 min,maintenance at 65% ACN for 10 min, and 65–5% ACN in 10 min.The typical operating conditions ... sol-vent B in 2 min, maintenance at 100% solvent B for 6 min, and linear gradient of 100–5% solvent B in 2 min. The col-umn was maintained at 30 °C and the ﬂow rate was 300 nLÆmin)1. Detection...

Tài liệu Báo cáo khoa học: parDtoxin–antitoxin system of plasmid R1 – basic contributions, biotechnological applications and relationships with closely-related toxin–antitoxin systems ppt

... point (+1) and the extended )10 and )35 are indicated (blue letters). The ribosome-binding site (RBS) and translation initiation codon(Met) of kis are underlined and shown in red. The N-terminal ... encoding a toxin and the other an anti-toxin, which are expressed at a low level. The toxin isneutralized in cells containing the plasmid by continu-ous production of the antitoxin. However, ... FEBSmonomers are involved in RNA binding, and disrup-tion of the orientation of the amino terminal hairpinby the C-terminal tail of the antitoxin inactivates thetoxicity of the protein.The crystal...

Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

... DBD–DBD interaction is important in theHSF–HSF and HSF–HSE interactions. In HSF DBD–HSE co-crystals, the protein–protein interface consistsof the helix 2 amino-terminus, the turn and the wing[15]. ... DNA-binding domain (DBD)at the amino-terminus and the oligomerization domain in the central region of the protein [1,4]. The HSFDBD belongs to the ‘winged’ helix-turn-helix family ofDNA-binding ... constitutively in cells and binds to and regulates the expression of genes without anyapparent stress intervention [1,3].HSF2 and HSF4 may not be directly involved in thestress response, but rather in...

Tài liệu Báo cáo khoa học: The miRNA-192 ⁄194 cluster regulates the Period gene family and the circadian clock doc

... miRNAs are single-stranded, nonprotein-codingRNA molecules, approximately 19–25 nucleotides in length. By binding to the complementary sites in the3¢UTRs of their target genes, they can induce ... cell line and NIH3T3+ indicates the cells overexpressing miR-192 ⁄ 194.(C) Graph showing the periodicity of Bmal1 mRNA levels in NIH3T3 cells with high levels of miR-192 ⁄ 194 and control cells, ... SW480,WiDr and EcoPack cells were cultured in Dulbecco’s modi-ﬁed Eagle’s medium supplemented with 10% fetal bovineserum and antibiotics. The serum shock to induce circadiancycling of NIH3T3 cells...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... thebinding of RNA-binding proteins to 3¢ UTR tran-scripts has been poorly described in plants.PUF proteins are a large family of RNA-bindingproteins found in all eukaryotes. These proteins ... redundantly in sperm–oocyte switch and germ stem cell maintenance [7,15]. However, these twoproteins show distinct patterning functions in the distalgerm line, independently affecting the number of cells in ... of neoblasts and impaired tissue regeneration [16]. In mammals,PUM2 is expressed in human germline stem cells [17],whereas the mouse homologs Pum1 and Pum2 are expressed in fetal and adult hematopoietic...

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