Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt
... A novel c-
N
-methylaminobutyrate demethylating oxidase involved
in catabolism of the tobacco alkaloid nicotine by
Arthrobacter
nicotinovorans
pAO1
Calin B. Chiribau
1
, Cristinel Sandu
1
, ... role in the
biodegradation of a n a lmost unlimited spectrum of natural
and man-made organic compounds, among them the
tobacco alkaloid nicotine. Perh...
... 5¢-GTTCACCACCAGAAGCT
GGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAG
GGTGCT-3¢;Abc, 5¢-CACAACGCCACCAACCATCAGA
CCGATGATAGCACCCTTGTTAGAACCCAC-3¢;Ab-
start, 5¢-GCGTAGGGTCGACATATGGACGCTGAATT
CCGTCACG-3¢;Abstop, ... 5¢-CCTGCCGAGCTCCTATTA
CACAACGCCACCAACCATCAG-3¢.
The PCR solution was prepared in the buffer supplied
with the enzyme, and contained Aba, Abb and Abcat
40 nm each, and the start and st...
... the synthesis of thermozeaxanthins and
thermobiszeaxanthins, which are the main carotenoids
of T. thermophilus [15]. The insertion of thermozeax-
anthins and thermobiszeaxanthins into the cell ... TSIPGVYACGDIVTYPGKLPLIVLGFGEAAIAANHAAAYAN-PALKVNPGHSSEKAAPGT 335
E.
coli 277 TSIPGVFAAGDVMDHI YRQAITSAGTGCMAALDAERYLD GLADAK 321
A.
pernix 283 TSIPGIFAAGDCTSMWPGFRQVVTAAAMGAVAAYSAY...
... 5¢-AAGTAGGCGGAGTATCGAAC-3¢ (sense) and
5¢-GAAAAAACGCGATCCTACTT-3¢ (antisense). Primers
for unmethylated DNA were: 5¢-GAAGTAGGTGGAGT
ATTGAAT-3¢ (sense) and 5¢-CAAAAAAACACAATCCT
ACTT-3¢ (antisense).
Caspase 3 activity
Cells ... Relative
fluorescence intensity was determined using imagemaster
2d elite software 4.01 (Amersham Bioscience, Uppsala,
Sweden).
Statistical analysis
Data in bar graphs...
... evolutionary aspects of invertebrate tachykinin
and tachykinin-related peptide
Atsuhiro Kanda, Kyoko Takuwa-Kuroda, Masato Aoyama and Honoo Satake
Suntory Institute for Bioorganic Research, Osaka, Japan
Tachykinins ... into tachykinin-related peptides, their receptors,
and invertebrate tachykinins: a review. Zool Sci 5,
533–549.
7 Satake H, Ogasawara M, Kawada T, Masuda K, Aoy-
ama M, Min...
... NADPH
were also obtained from Sigma. Sephadex G-25 was pur-
chased from Pharmacia (Uppsala, Sweden). All the other
materials were of analytical grade and obtained from
Beijing Chemical Plant (Beijing, ... First,
the substrate-binding site should match the size and
shape of the substrates, and second, incorporation of
an imido-group increases the stability of transition
state...
... Thomas-Oates, J.E. & Brade, H. (1994) Preparation
and structural analysis of oligosaccharide monophosphates
obtained from the lipopolysaccharide of recombinant strains of
Salmonella minnesota ... KOH. The
lipo-oligosaccharide was also analyzed after acid treatment,
attained by mild hydrolysis with acetic acid, to obtain
information on the nature of the phosphate and acyl...
... [6,8,27] or to
any other sequences available in FASTA and BLAST
database programs at the DNA Data Bank of Jap an.
Recently, we reported the cloning and s equencing of the
gene encoding 4-amino-3-hydroxybenzoate ... resting
cells of a mutant, strain Y-2, of the aniline-assimilating
Pseudomonas sp. strain AW-2 [20].
Results
Spectral changes during metabolism of 4-amino-3-...
... that pattern-based
inference is also necessary. The relations of adjacent
structure can infer the relations of separated structure
if there are certain linguistic indicators in the local
context. ... training data (with true entities) where 6
types and 18 subtypes of relations are annotated. We
use 75% of it to train SVM classifiers and the
remaining to evaluate resul...
... VI)
showed a linear rate up to 30 min (Fig. 4A) . After further
incubation it deviated from the linearity and became
saturated at 60 min. Titration of helicase activity with
increasing amounts of the ... which catalyse
the DNA unwinding in an ATP-dependent manner and
thereby act as an essential molecular tool for cellular
machinery [2–6]. All helicases exhibit intrinsic DNA-
d...