... nm,comparable to the inhibition of the aminopeptidasecathepsin H (Ki¼ 30 nm ). In comparison with othercystatins, cystatin F is a rather slow binding inhibitor of the cathepsins, characterized ... activation of cystatin F wehave studied the in vitro kinetics of the interactionbetween cystatin F and several cathepsins, as wellas their intracellular localization in promonocyteU937 cells, using ... Cystatins are generally rather non-selective inhibitors. An interesting feature of cystatinF is the 100-fold stronger inhibition of cathepsins F,L, and V than of cathepsin S. Cathepsin S is...