Tài liệu Báo cáo khoa học: Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases pptx

... belonging t o class I of the superfamily of bacterial, fungal, and plant heme peroxidases. In Fig. 5 the simplified inferredFITCHtree, is presented, and in Fig. 6 the same tree in a simplified ... feature in this region. The iron of the prosthetic heme group is invariantlyco-ordinated by the a bove essential proximal h istidine(Fig. 4A,C,D). However, in all C-domains of catalase– peroxidases, ... vent in the g ene f or catalase–peroxid aseoccurred in the later phase of evolution, in which the indi-vidual specificities of the peroxidase families distinguishedwere already formed. Evidence...

Tài liệu Báo cáo khoa học: Fermentative lifestyle in yeasts belonging to the Saccharomyces complex ppt

... A.Clade StrainAntimycin A concentration (lM)Richmedium:5Syntheticminimalmedium:0.5–25Syntheticminimalmediumplus lysine and glutamic acid:0.5–25Syntheticminimalmediumplusacetoin:0.5–25Saccharomyces ... on synthetic minimalTable 2. (Continued).Clade StrainAntimycin A concentration (lM)Richmedium:5Syntheticminimalmedium:0.5–25Syntheticminimalmediumplus lysine and glutamic acid:0.5–25Syntheticminimalmediumplusacetoin:0.5–25Klo. ... strict anaerobic condi-tions: yeast species in red grow both on rich and on synthetic minimal medium within7 days; species in blue grow on rich med-ium within 7 days and on synthetic minimalmedium...

Tài liệu Báo cáo khóa học: TbPDE1, a novel class I phosphodiesterase of Trypanosoma brucei pdf

... moiety of the catalytic domain, but still containing its corepart (Lys321–Thr620) was inactive in yeast, and wasexpressed in E. coli as an inactive polypeptide in the form of inclusion bodies ... or with the trypanosomal TbPDE2 family.Outside of the catalytic domain, sequence similarity decrea-ses, within 10–40 amino acids at the N-terminal side of the domain, and within 15 amino acids ... amino acid identity, including all functionallyimportant residues, with the catalytic domains of humanPDEs. A fragment of TbPDE1 containing the catalyticdomaincouldbeexpressedinactiveforminEscherichia...

Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf

... using the main chain of residues Thr23fiAsn90 in i soform 337 and residuesThr54fiMet121 in CfAFP-501. (B) Overlap of isoforms 337 and 501using the main chain of r esidues 4–33 in both proteins.Ó ... This isprobably due to the difficulty in folding the protein, ratherthan suggesting that this face of T mAFP interacts with the ice surface.It is important to distinguish whether the m utationsdisrupt ... proteins [47]. Additional rigidity i n T mAFP arisesfrom the eight disulphide bridges in the core of the protein.Two studies by Daley & Sykes examined the conforma-tion of the Thr side-chains...

Tài liệu Báo cáo khoa học: An engineered disulﬁde bridge mimics the effect of calcium to protect neutral protease against local unfolding docx

... binding-site III(Fig. 1). To assign the contribution of bound calciumions at calcium binding-site III, the variant W55F, car-rying a mutation in the respective binding site, wasincluded in ... disulﬁde bridge in G8C/N60C mimics the occupation of calcium binding-site IIISimilarly to the occupation of calcium binding-site III, the introduction of the disulﬁde bridge G8C ⁄ N60Cinto the enzyme ... attention ispaid to the role of calcium ions. For this reason, anenzyme variant with a mutation in the vicinity of cal-cium binding-site III (W55F) is included in the studies. The results indicate...

Tài liệu Báo cáo khoa học: "Exploiting Readymades in Linguistic Creativity: A System Demonstration of the Jigsaw Bard" docx

... proposes has the ring of linguisticauthenticity; because this authenticity is rooted in another, more literal context, the Bard also exhibitsits own Duchamp-like (if Duchamp-lite) creativity.We ... Duchamp’sFountain being considered one of the most influ-ential artworks of the 20th century – we shall showthat the notion of a linguistic readymade has sig-nificant practical merit in the realms of ... responsibility to be “scrupu-lous” in their use of language by “simply throwing[their] mind open and letting the ready-madephrases come crowding in . For Orwell, words justget in the way, and...

Tài liệu Báo cáo khoa học: "Language Resources Factory: case study on the acquisition of Translation Memories" potx

... outthis process: acquisition of the data, its alignment and its conversion into the desired format. Theseare provided by WSs available in the registry.First, we use a domain-focused bilingualcrawler13 in ... can easily ﬁndexamples that will help them during the designphase, being able to reuse workﬂows (or parts of them) and thus avoiding reinveinting the wheel.4 Using the tools to work with NLPAll ... InteroperabilityInteroperability plays a crucial role in a platform of distributed WSs. Soaplab deploys SOAP10WSs and handles automatically most of the issuesinvolved in this process, while...

Tài liệu Báo cáo khoa học: Evolutionary relationships of the prolyl oligopeptidase family enzymes docx

... P1 subsite of the inhibitor. The most interesting amino acid at the S3 subsite isCys255, because it is responsible for pig POP inhibitionby bulky thiol reagents. F. meningosepticum, which has ... antitrypanosomaldrugs in widespread use are OB inhibitors [10]. In addition,inhibition of DPP IV has been proposed as a therapeuticapproach to the treatment of type 2 diabetes as this enzymeis involved in the ... hydrolyzes peptides at the carboxyl side of the proline residue, DPP IV liberates dipeptides where the penultimate amino acid is proline, OB cleaves peptides atlysine and arginine residues and ACPH...

Tài liệu Báo cáo khoa học: "Seeing stars: Exploiting class relationships for sentiment categorization with respect to rating scales" doc

... (respecting the orig-inal labels of the training instances) that minimizestestwhere is monotonically increasing (we choseunless otherwise specified) and is atrade-off and/ or scaling parameter. (The ... standard classifier?A: Our focus is on investigating the utility of simi-larity information. In our particular rating-inferencesetting, it so happens that the basis for our pair-wise similarity measure ... explicit item and label similarityinformation to increasingly penalize the initial clas-sifier as it assigns more divergent labels to similaritems. In this paper, we only report supervised-learningexperiments...

Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf

... solid line), 60 min (thin long-dashed line),120 min (thin short-dashed line), 240 min (thin dotted line) and 360 min (thick dotted line) of incubation, the spectrum of enzyme-bound cobalamin ... lacking the ade-nine ring in the upper axial ligand; a model of damagedcofactors) for free adeninylpentylcobalamin (AdePeCbl)(an inactive coenzyme analog containing the adeninering in the ... resulting in the irreversible inactivation of the enzyme.This apparent inconsistency was resolved by ourﬁnding of the rapid reactivation of glycerol-inactivatedenzymes in permeabilized Klebsiella...

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