Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... mediator of lethal sepsis, triggering the production of proinﬂammatory cytokines and liverinjury [13].Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... observed in the absence of changes in protein expression in rabbit he-patocytes [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing phosphorylation of the isoforms ... expressed in the liver, is primarily involved in the oxidative metabolism of xenobiotics and is capable of the metabolic activation of numerous procarcino-gens, including aﬂatoxin B1 [21]. In the...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... control the amount of intracellu-lar CagAPY. Cortactin, ezrin and vinculin are all Srcsubstrates, and Src inactivation causes dephosphoryla-tion of these proteins and is crucial for triggering ... distribution,making them unsuitable for the investigation of b-cate-nin signalling [29]. In support of this aspect, usingMadin–Darby canine kidney cells that expressE-cadherin and b-catenin without ... (11). In this manner, the bacteria could access integrins and inject CagA (12). Injected CagA can then induce the massive disruption of cell junctions(13) and a loss of cell polarity (14). The induction...

Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

... were obtained in ChIP DNA, indicating that the binding of putative CERK-PPRE to PPARb isspeciﬁc. Furthermore, the interaction between PPARb and CERK was conﬁrmed by an EMSA using biotin-labeled ... culturedhuman keratinocytes [14]. In this study, we examined the interaction betweenCerK and PPARb, and its role in regulating keratino-cyte survival. We report that in a mouse keratinocytecell line upregulation ... Ceris incorporated, hydrolyzed to sphingosine and thenrecycled into intracellular Cer itself, and that accumu-lation of Cer contributes to the induction of apoptosis[20]. However, the signaling...

Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... amounts of proteinfrom 3Y1-Ad12 cell lysates confirmed the absence of cathepsin D in nontransfected cells, and the presence of both the 31 kDa mature cathepsin D and the 45 kDa procathepsin D in cathepsin ... bafilomycin and the cathepsin D inhibitor pepstatin Apartially inhibited, both in vivo and in vitro, the cAMP response to choleratoxin. This cathepsin D-dependent action of cholera toxin under the con-trol ... con-trol of endosomal acidity was confirmed using cellular systems in whichmodification of the expression levels of cathepsin D, either by transfection of the cathepsin D gene or small interfering...

Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

... AP1 DNA binding activity increasedwithin 1 h following EGF treatment and peaked at 3 hfollowing the treatment (Fig. 5A). We also examined the effect of PD98059 on this binding activity and, asexpected, ... transcription, were increased in response toEGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinasekinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNA binding, Bcl-XLexpression, ... each binding reaction, 35 fmol of the 32P-labeled oligonucleotide(50,000–100 000 c.p.m.) was incubated with 5 lg of the nuclear protein extract at room temperature for 20 min in the binding...

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase Alison ... with the K22V and K22E mutantproteins. This was interpreted as a lack of fosfomycin binding to these mutant proteins [12]. Binding of fosfomycin to either wild-type MurA or the K22 mutant proteins ... doi:10.1111/j.1432-1033.2004.04196.x the positively charged side chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins.However,...

Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S conﬁguration of isostere in binding properties of HIV-1 protease inhibitors docx

... large in uence onconformation of inhibitor main chain. Thus, differentinhibitors differ not only in t he binding afﬁnity, bu t a lso in the degree o f freedom of the inhibitor inside the binding tunnel. ... 2.0 A˚. The overall layout of the inhibitor in the pr otease binding site is very similar for all the compared inhibitors. The sidechains of all the inhibitors are placed similarly in theirpockets, ... 271) 4453Inhibitor binding. The inhibitor OE b inds into the p rotease binding tunnel in an e xtended c onformation in two oppositedirections (Fig. 4). The ﬂaps are locked over the inhibitor...

Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafﬁcking pdf

... measuring the binding of [125I]Streptavidin. Specific binding at each point was nor-malized with reference to specific binding before the incubation at37 °C (0-min point) to derive the fraction of ... biotinylated Ig remainingon the cell surface was then quantified by measuring the binding of [125I]Streptavidin. In the absence of bTSH, a veryweak internalization of the TSHR is detected ... region of the TSHR. The enzyme involved in the maturation of the TSHR sharessimilarities with the ADAM (A Disintegrin and Metallo-protease) family of metalloproteases [12,18]. The cleavage and...

Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

... opening and cell swelling, an event thatis probably reduced in extent by CsA. CsA speciﬁcallyinhibits PTP opening by binding to cyclophilin D in the matrix and on the inner surface of the IM ... decreasing the activity of caspase-3 in the three cell lines.Effect of HSS knockdown on CCCP-inducedapoptosisTo further elucidate the functional role of HSS in CCCP-induced apoptosis, the expression ... cloning the PCR fragments into the His-tag vector, pET-15b. The recombinant proteins were generated in the E. coli strainOrigami (DE3), and the N-terminal His-tagged proteinswere puriﬁed using...

Tài liệu Báo cáo khoa học: Simpliﬁed yet highly accurate enzyme kinetics for cases of low substrate concentrations ppt

... that the former is closer to a SIM than the latter. Using these plots and having measured the concentration of EIIAÆP, the investigator can read the values of the remaining eight concentrations off the ... ½HPrtot¼~z2þ~z3þ~z8þ~z9;½EIIAtot¼~z3þ~z4þ~z10þ~z11;½EIICBtot¼~z4þ~z5þ~z12þ~z13ð22ÞUsing these, we can express~z6,~z8,~z10 and ~z12 in terms of the remaining state variables, substitute these expressions in the original model, and obtain the nine-dimensionalODE ... approaches aiming at understanding the complexinteractions in living cells is increasing.Materials and methods The PTS modelHere, we present the PTS model [8] in detail, list the linearconservation...

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