... 3¢-end of Oligo II could be ligated to the BstEII site. Similarly, the5¢-ends of Oligos III, V, VII and IX were designed to ligateto the BstEII, BglII, EagI and NcoI sites, respectively, and their ... form of rat HO-1 [37], H64L mutant of myoglobin [38], maize ferredoxin type III [39], maizeFNR [40], a truncated form of human CPR [41], and ratBVR [42].Heme binding and preparation of heme–GmHO-1To ... the 5¢-ends of Oligo IV, VI, VIII and X. The 3¢-end of Oligo X had asequence designed to ligate to the HindIII site. To completethe GmHO-1 expression vector pMWGmHO-1, double-stranded Oligo...