... involvement of src and PDGFR
in tyrosine phosphorylation of S6K further, we studied
the effect of inhibitors on tyrosine phosphorylation of
S6Ks. As expected, genistein, a broad-range tyrosine
kinase ... (A) Deletion of the N-terminus leads to a loss of phos-
photyrosine in S6K. Hek293 cells were transiently transfected with WT and truncated mutants of S6K (S6K1, S...
... dis-
tribution of the energy between the overall tumbling
and the internal mobility, and is indicative of the
degree of internal restraint of each
15
N–
1
H vector
motion. In Fig. 4, most of the points ... graph of R
1
, R
2
and {
1
H}–
15
N NOE val-
ues along the sequence of the TTF1 HD at 11.7 T and
286 K.
Fig. S2. Plot of J(0.87x
N
)–J(0) correlation from
15
N
relax...
... Pro, Val or Lys, c-carboxylation
of Glu, glycosylation of Ser or Thr, bromination of
Trp, sulfation of Tyr, cyclization of N-terminal Gln,
and epimerization of several different residues. It ... structure of conomarphin
was similar to those of other conotoxin cDNAs. The
cDNA-encoded precursor of conomarphin consisted of
a conserved M-superfamily signal peptide of 25 re...
... and the CFU counted. The per-
centage of surviving CFU was calculated by the formula
N ⁄ N
control
· 100, where N and N
control
were the numbers of
CFU obtained after 3 h of incubation with and ... (2006)
Secretion of serine peptidase by a clinical strain of
Candida albicans: influence of growth conditions and
cleavage of human serum proteins and extracellular
matrix co...
... concentration of Rep245 pre-
pared in 10 mm sodium phosphate (pH 7.4) was 5 lm. CD
spectra were recorded at 60, 70 and 80 °C between 190 and
260 nm with a step increase of 0.2 nm, and a bandwidth of
1 ... presence of 0.2 mm
dNTPs and 0.5 U of Taq DNA polymerase (Promega).
To analyze the effect of temperature on primase activity,
Rep245 and Rep516 proteins were incubated...
... software swiss-pdbviewer and
visual molecular dynamics [49].
Acknowledgements
We thank G. Magni and S. Ruggieri for helpful com-
ments and suggestions and T. Begley (Department of
Chemistry and ... electrophoresis of the PCR product indicated the
presence of two distinct bands between 1000 and
1100 bp of roughly the same extent (not shown). Clo-
ning and nucleotide seque...
... catalytic proper-
ties of HpSDH and the effects of pH and temperature
on HpSDH. The results show that HpSDH has a k
cat
of 7.7 ± 0.9 s
)1
, K
m
of 0.148 ± 0.028 mm and
k
cat
⁄ K
m
of 5.2 · 10
4
m
)1
Æs
)1
toward ... shikimate, k
cat
of 7.1 s
)1
and K
m
of 0.182 mm toward NADP, k
cat
of 5.2 s
)1
and K
m
of 2.9 mm toward NAD. The optimum pH of the
enzyme activity i...
... bonds between NH of Thr5 and
CO of Arg2, as well as NH of Arg2 and CO of Thr5.
Structure of cyclic hexapeptides – cYT peptide. The
NMR data of the cYT peptide wer e indicative of its flexible
nature. ... structure of peptide cER
consists of two b-turns, located at the N- and C-termini. A
comparison of the b-turn structure of cER with the similar
region in the crysta...
... Fragments of HSQC and HMBC
spectra of compound 1. Labels illustrate
assignment of the amide linkage between the
amino group of Asp and the carboxyl group of
GalA residue E.
Scheme 1. The structures of ... min), dried and analyzed by GC-MS with the standards
prepared from
D
-GalN and (R)- and (S)-2-BuOH.
The absolute configuration of
L
-aspartic acid was deter-
mined by...
... Jingyue Yang
1,2
and Richard W. Gross
1,2,3,4
1
Division of Bioorganic Chemistry and Molecular Pharmacology, and Departments of
2
Medicine,
3
Chemistry and
4
Molecular Biology
and Pharmacology, ... tissue w hich will
further the understanding of the role of iPLA
2
sinenergy
storage and utilization disorders of lipid metabolism such as
diabetes, obesity and atherosclero...