Tài liệu Báo cáo khoa học: Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation doc
Tài liệu Báo cáo khoa học: Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation doc
... compilation ª 2006 FEBS Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation Bernard ... affin-ity than with Mst1 (Fig. 3D).Both phosphorylation of Sav and its increase inabundance were dependent of the ability of Sav and Mst to interact. Deletion of the coiled-coil domain, and thus ... domains of both proteins. hSav was alsoable to homodimerize, but this did not require its coiled-coil domain. Coex-pression of Mst and hSav led to phosphorylation of hSav and also increasedits...
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Tài liệu Báo cáo khoa học: Association of feather colour with constitutively active melanocortin 1 receptors in chicken docx
... that allows the receptors to fall into aconstitutively active state without binding to a ligand arenot automatically conserved within the MC receptors orwithin the MC1 receptors. Therefore, we ... region of each cMC1 receptor variant. To facilitatecloning and establishment of orientation of the PCR-amplified DNA fragments, recognition sites for HindIII and BamHI were introduced into 5¢ and ... Glu to Lys mutation in to thehuman MC1 and MC4 receptors to investigate the pharma-cological impact of these mutations.ExperimentalReceptor clonesOligonucleotide primers were designed to...
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Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf
... biphasic, with the best fit obtained with a two binding sitemodel, comprising a first site with binding stoichiome-try (n) of 0.3 ± 0.2 and a binding constant (Kd )of 3.4 ± 4 · 10)9m and a second with ... value of the protonableABFig. 3. Ensemble of the backbone traces of the 20 lowest energyconformers of the solution structure of (A) NarG(1–15) and (B)NarG(1–28).ABFig. 2. (A) Sequences of ... strong binding of NarG peptides to NarJ. To predict the interaction surface between NarJ and NarG, we performed a docking experiment in anab initio mode using haddock software. Six of the 10best...
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Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx
... progress in Lea Sistonen’s labora-tory in order to unravel the specific targets of HSF1 inspermatogenesis and to estimate the proportion of com-R. Abane and V. Mezger Role of the HSF family ... instead of decreasing at 6 weeks. Such a high expression inhibitsthe maturation of olfactory sensory neurons and leads to a reduction in the thickness of the olfactory epithe-lium via cell ... Lack of maternalHeat Shock Factor 1 results in multiple cellular and developmental defects, including mitochondrial damage and altered redox homeostasis, and leads to reducedsurvival of mammalian...
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Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efficacy pdf
... behavior of thissequence could be due to enhanced interactions with Dicer and a reduced need to interact with TRBP,relative to the other sequences.Effect of guide strand 5¢ end mismatch on TRBP and ... duplex and directs binding of Dicer-2 to the other, less stable, end, and hence theguide strand is selected through interaction of its 5¢ end with Dicer-2 [18,19]. While the functions of the ... homolog of R2D2, senses siRNAasymmetry [4]. To ensure maximal specific silencing of the intended target, loading of the appropriate guidestrand into the RISC is critical. Improved understand-ing of...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... conditions. All of the work with mice conformed to guidelines approved bythe Institutional Animal Care and Use Committee of theUniversity of Tokyo.Construction of the transgeneTotal RNA was ... mouseCorrespondenceH. Ichise, Laboratory of Gene Expression and Regulation, Center for ExperimentalMedicine, Institute of Medical Science,University of Tokyo, 4-6-1 Shirokanedai,Minato-ku, Tokyo 108-8639, JapanFax: ... Yoshida and Hirotake IchiseLaboratory of Gene Expression and Regulation, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, JapanAs an indispensable component of...
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx
... similar to that of Cg-Clp1.Expression of Cg-Clp2 transcripts duringdevelopment and in adult tissues To gain insights into possible physiological functions of Cg-Clp2, determination of its tissue ... Double-stranded cDNA from oyster mantle edges was ligated to adaptors, and 25 ng of this template was used to PCRamplify 5¢- and 3¢-RACE fragments using adaptor-specificprimers and gene-specific ... growthfactors [8,9,28]. As was observed for its murinehomologue (YM-1), which behaves as a chemotacticcytokine that recruits cells to sites of inflammation and promotes eosinophilia around larvae of...
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Tài liệu Báo cáo khoa học: Oligomerization of the Mg2+-transport proteins Alr1p and Alr2p in yeast plasma membrane pptx
... mm EDTA to removeextracellular bivalent cations and then with EriochromeBlue ⁄ buffer to remove EDTA. Cells were resuspended to an D600 of 0.9–1.0 and treated with 10 lgÆlL)1digitonin atroom ... treatment with lambda phosphatase(kPP; New England Biolabs, Ipswich, MA). With the use of 0, 200, and 400 units of kPP, the samples were incubatedfor 30 min at 30 °C. The reactions were stopped with the ... essential for uptake of Mg2+into yeast cells. Homologs of Alr1p are restricted to fungi and someprotozoa. Alr1-type proteins are distant relatives of the mitochondrial and bacterial Mg2+-transport...
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Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx
... Instituto Leloir, Buenos Aires, ArgentinaTrypanosoma cruzi, the etiological agent of Chagas’disease, is a parasitic protozoan with a digenetic lifecycle involving an insect vector and a mammalian host. ... 2006 FEBS to synthesize putrescine, as a result of the absence of ornithine and arginine decarboxylases (ODC and ADC) [7–10], which catalyse the first steps of bothpossible pathways of putrescine ... attributedneither to a deficient uptake of the amino acids bythe parasites [12] nor to the presence of ODC or ADCinhibitors in the protozoan internal medium [7,10].Studies carried out in order to correlate...
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Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt
... interactions with 14-3-3 and factor activating ExoS (FAS) protein cofactors[24–26].As this interaction is necessary for the ADP-ribosyla-tion activity of ExoS, and more intriguingly appears to be ... astrategy of single amino acid site-directed mutagenesis of the cofactor interaction domain within ExoS.Various single mutant ExoS proteins were tested fortheir capacity to interact with 14-3-3 and ... loose and semidetached cytotoxic cells were washed free frombacteria and transferred to a new Petri dish and incu-bated overnight with medium containing gentamicin.Bacterial growth of each...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM