Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc
... FEBS 3171
Inhibitory effects of nontoxic protein volvatoxin A1
on pore-forming cardiotoxic protein volvatoxin A2
by interaction with amphipathic a-helix
Pei-Tzu Wu
1
, Su-Chang Lin
2
, Chyong-Ing ... demonstrated
that volvatoxin A consists of volvatoxin A2 and volvatoxin A1, and the
hemolytic activity of volvatoxin A2 is completely abolishe...
... evolutionary selection of protein sequences is the
catalytic competence of the enzyme active site. The pre-
cise orientation of the functional residues is maintained
by a network of interactions ... a linear gradient of 0–1 m NaCl. The fractions
containing the protein were pooled and precipitated by
addition of ammonium sulfate to a concentration of 75%.
The precipitated...
... attractive for further consi-
deration.
Potentiation of the antimicrobial activity of HN
and related compounds by ATP
Inflammation caused by infection or wounds triggers
the stimulation of neutrophils, ... Medicine, University of Ottawa, Canada
Histones are highly conserved proteins that play a key
function in the packaging of DNA within eukaryotic
cells during their division [1]...
... FLAG-Mxi1-SRb under the control of the TET ON
promoter. We monitored induction as well as expres-
sion levels of the isoforms by immunoprecipitation
with an FLAG antibody followed by anti-FLAG wes-
tern ... R, Thomson
CT, Sacchettini JC & DePinho RA (1994) Evolutionary
relationships and functional conservation among verte-
brate Max-associated proteins: the zebra fish homolog...
... as one of the main means of
regulation [8]. The best characterized are the cystatins,
which comprise a superfamily of evolutionarily related
proteins, each consisting of at least one domain of
100–120 ... dithiotreitol concentrations above
30 mm (Fig. 1B). In addition, the effect of increasing the
concentration of reductant on cystatin F was followed
by electrophoresis under...
... Parmeggiani,
A. (1978) Modification of elongation-factor-Tu–guanine-nucleo-
tide interaction by kirromycin. A comparison with the effect of
aminoacyl-tRNA and elongation factor Ts. Eur. J. Biochem. ... Parmeggiani, A. (1977) Effect of kirro-
mycin on elongation factor Tu. Location of the catalytic center for
ribosome-elongation-factor-Tu GTPase activity on the elongation
factor...
... Angiotensin-
converting enzyme 2 is an essential regulator of heart
function. Nature 417, 822–828.
6 Allred AJ, Donoghue M, Acton S & Coffman TM (2002)
Regulation of blood pressure by the angiotensin ... inhibition by the classical ACE inhibitors
[1,2]. After the elucidation of the crystal structure of
testicular ACE (tACE), [19] a model of the active site
of ACE2 was desc...
... that inhibition of LTB
4
and PAF biosynthesis by
AACOCF
3
occurs without concomitant inhibition of AA
release. Generation of the common precursor pool by
CoA-IT occurs by the transfer of AA from ... determined
in a portion of the isolated fractions by liquid scintilla-
tion counting using a Beckman liquid scintillation
counting system (Fullerton, CA, USA). Portions of
PtdC...
... consumption of excess fat. However, the combination
of persistent ANT inhibition by LCACs with constant
activation of AMPK may have some adverse effects,
because stimulation of b-oxidation ... cellular function by direct
effects on a variety of enzymes and induction of
apoptosis [4].
Tight regulation of intracellular concentrations of
free LCACs by acyl-CoA-bindi...
... genera-
tion of H
2
O
2
in spores [9,15–18].
In this work we have gained more information on
the cellular targets and the mechanism of action of vis-
cotoxins, by examining the interaction of VtA
3
with
fungal ... permeabilization
[3,16]. To further explore the interaction of the most
cytotoxic viscotoxin isoform, VtA
3
, with biological
membranes and obtain information...