Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of

Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf

... oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level Yongzhao Zhang 1 , Kazumichi Furuyama 1 , Kiriko Kaneko 1 , Yuanying Ding 1 , Kazuhiro ... regula- tion of HO-2 expression in human cells under hypoxia. In the present study, we have analyzed the effect of hypoxia on the...

Ngày tải lên : 19/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Pyruvate reduces DNA damage during hypoxia and after reoxygenation in hepatocellular carcinoma cells pptx

... protective agent against DNA damage under conditions of tumor hypoxia. Many strategies for cancer therapy are based on increases in the number of DNA strand breaks (e.g. radiotherapy and alkylat- ing agents). ... extracellular content and the increase of its uptake induced by hypoxia show that alteration of pyruvate metabolism takes place. The greater uptake might be due...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Hypoxia downregulates farnesoid X receptor via a hypoxia-inducible factor-independent but p38 mitogen-activated protein kinase-dependent pathway doc

... expression, we used the following validated siRNAs: Hs_MAPK14_6_HP validated siRNA against human p3 8a; Hs_MAPK3_7_HP validated siRNA against human ERK1; and Hs_MAPK1_10_HP validated siRNA against ... denatured at 90 °C, cooled for annealing, and used to knock down HIF- 1a. Similarly, the siRNA against HIF- 2a was prepared using the sense (5¢-CAGCAUCUUUGAUAGCAGUdTdT- 3¢) and ant...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Hypoxia-inducible factor-1a blocks differentiation of malignant gliomas pdf

... corresponding to the targeting siRNAs is: HIF- 1a: 5¢-TCGACAAGCTTAAGAAAGA-3¢; VHL: 5¢-CCAAGACACCTCGAGAAT-3¢ (Ribobio, CN). The scrambled siRNA sequence used as negative control is 5¢-GAGUAGAAGAUUCAAGCUU-3¢ ... Statistical evaluation revealed that the amount of HIF- 1a was signiﬁcantly increased in parallel with increasing glioma grade (Fig. 7B). The percentage of HIF- 1a- posit...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding afﬁnity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx

... al. [34]. The protein conce- ntration was calculated based on the values obtained for alanine, threonine, glycine, valine, phenylalanine and aspar- tate. The analysis, starting from protein hydrolysis, ... surface of the structural unit that contains the catalytic site, rather than on an auxiliary domain [9]. These substrate binding sites are located at a certain distance fr...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: histone H3 lysine 4 methyltransferases from yeast to human pptx

... overexpressed in prostate cancer. The implication of HMTs and demethylases in cancer and human diseases has led to the idea of utiliz- ing them as therapeutic targets. In fact, biguanide and bisguanidine ... and bisguanidine polyamine analogs inhibit the demethy- lase, LSD1, in human colon carcinoma cells. Inhibition of LSD1 facilitates expression of the aberra...

Ngày tải lên : 16/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... protein seems to have several functions, both intracellularly and extracellularly. By reducing the S10 0A4 level in a human osteosarcoma cell line, and implementing these in mice, the capacity to ... I, Iwata K, Yamashita K & Hayakawa T (1992) Matrix metalloproteinase 9 (92-kDa gelatinase ⁄ type IV collage- nase) from HT1080 human ﬁbrosarcoma cells. Puriﬁca- tion and act...

Ngày tải lên : 18/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

... polyclonal antibodies raised against each of the WhiB proteins. The absorbance obtained in the positive control (where the antibody used was raised against that particular antigen) was set to ... pH and redox conditions [1]. Among several mechanisms for resistance to intracellular killing, the scavenging of free radicals and the reactivation of degenerated proteins d...

Ngày tải lên : 18/02/2014, 12:20

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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

... and functional properties of RNase and participates in degradation of IL-1b mRNA Danuta Mizgalska 1 , Paulina We˛ grzyn 1 , Krzysztof Murzyn 2 , Aneta Kasza 1 , Aleksander Koj 1 , Jacek Jura 3 , Barbara ... following antibodies were used: polyclonal antibodies against lamin C (Abcam, Cambridge, MA, USA) for the nuclear fraction, polyclonal antibodies against GAPDH D. Mizgalska et al...

Ngày tải lên : 18/02/2014, 14:20

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Tài liệu Báo cáo khoa học: PC1⁄3, PC2 and PC5⁄6A are targeted to dense core secretory granules by a common mechanism doc

... triad of amino acids aspartic acid, histidine and serine, and a stabilizing P-domain involved in the binding of Ca 2+ [1]. The C-terminal domains of the PC enzymes exhibit the least amount of homology ... extent of intracellular protein retention and forskolin-stimulated secretion of the Fc protein thereby constitute the base- line for analyzing potential granu...

Ngày tải lên : 18/02/2014, 16:20

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