... enhance anticancer drug activity.
Our recent results have shown that the activation of
JNK induced by EGFR-TKI AG1478 is critical for
the apoptotic action of AG1478 against the NSCLC
cell line PC-9 ... Members of the KIP
family can inhibit the catalytic activity of
CDK2, 4 and 6 Members of the INK4 family
are speci c inhibitors of the cyclin
D–CDK4 ⁄ 6 complex It is...
... analyzed
using cellquest software (Becton Dickinson).
Isolation of mitochondria
The isolation of mitochondria was performed according to
the instructions for the Mitochondria ⁄ Cytosol Isolation Kit
for Cultured ... to protons and causes dissi-
pation of the proton gradient across the IM. CCCP
also uncouples the transfer of electrons through the
electron transfer chain f...
... Diagnostics) and the
following PCR primer sets: 5¢-GGAGATCTCCAGTGA
TATCGACCA-3¢ and 5¢-ACGGCTTCTACGGATCGAA
ACT-3¢ for PPARc ,5¢-AAGACAGCTCCTCCTCGAAGG
TT-3¢ and 5¢-TGACCAAATCCCCATTTACGC-3¢ for ... aP2,
5¢-ATCCATGGATGGACGGTAACG-3¢ and 5¢-CTGGA
TCCCAATACTTCGACCA-3¢ for LPL, 5¢-TGGGTTGG
CTGCTTGTG-3¢ and 5¢-GCGTGGGCAGGATGAAG-3¢
for SCD, 5¢-GATGTGGAACCCATAACTGGATTCAC-3¢
and 5¢-GGTCCCAGTCTCATTTAGC...
... example of this phenomenon is offered
by an enzyme-catalyzed reaction where the function is
the conversion of one metabolite into another: in this
case, the formation and dispersion of the complex of
the ... The concentrations of the molecules depicted in boxes are the state variables in the model [8],
whereas the concentrations of the remaining molecules a...
... in the culture
medium, which corresponds to changes in intracellular
collagen-I protein content. In addition, quercetin
prevented an a-defensin-induced increase in collagen
content in the culture ... image of immunoblot against
active ⁄ dephosphorylated b-catenin from three experiments.
WO siRNA Control siRNA
β-catenin siRNA
Con D1 D2 Con D1 D2 Con D1 D2
Con D1 D2 Con
D1 D2
Con D1
D2...
... of
incubation at 37 C, the reaction was quenched by the
addition of 0.2 mL of a solution containing 11 m formic
acid and 5 m HCl, and heated to 90 C for 10 min. The
acidification of the reaction mixture ... nmolÆmin
)1
. (B) The dependence of hMS activity on
NADPH concentration, illustrating the optimal concentration of
NADPH required for reductive methylation (react...
... Special Pro-
ject Research on Cancer-Bioscience 17014020 from the
Ministry of Education, Culture, Sports, Science and
Technology of Japan, a grant from the program
‘Research for the Future’ of the ... show c- myc
activation, but the dominant active mutant form of
Ras is less frequent. Gene amplification and activation
of EGFR occur in more than one third of cases, the...
... iron accumulation-2
(NBIA-2) is characterized by the disruption of cellular
mechanisms leading to the accumulation of iron in the
basal ganglia. Mutations in the gene PLA2G6 were
recently described ... been cloned in which
mutations cause parkinsonism in a mendelian fashion
[ 3–8 ] (Table 1).
Classically, the approach taken to the study of
genetic forms of PD has relied...
... TRAIL protein for an additional
12 h under normoxic and hypoxic conditions. Hypoxic
conditions inhibited significantly the apoptosis of
chondrocytes induced by cotreatment of the cells with
the ... carti-
lage chondrocytes. Arthritis Rheum 27, 129 0–1 299.
29 Marcus RE (1973) The effect of low oxygen concentra-
tion on growth, glycolysis, and sulfate incorporation by
arti...