Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf
... (C-terminal)-containing fragment ions. Thus, for the + 1.0 Da sam-ple (Fig. 8B), the N-terminal fragment ions show littleincrease in mass with increasing size until Asn67, with this increase of ... squaresrepresent the distribution 1 Da higher. To determine the mass increase with increasing time of deamidation(pH 9.6), the best fit of calculated intensity values(squares) was determined (Fig. 9B–E). The ... precision of locating such sequence errors or PTMs is depen-dent on obtaining fragment ion masses representingnearby dissociations on either side of the error; in the unusual Fig. 4 case of...
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Tài liệu Báo cáo khoa học: Oxidized elafin and trappin poorly inhibit the elastolytic activity of neutrophil elastase and proteinase 3 pdf
... that (a) the observed inhibition is lower than that with the theoretical inhibitor, regardless of the enzyme, the inhibitor and the state of oxidation of the latter, indi-cating that elastin does ... inhibition of Pr3 by the oxidized inhibitors.While the above data are in overall agreement with the results obtained using synthetic substrates, theyalso indicate that elastin hinders the inhibition ... yields. The two full-length recombinant inhibitors were foundto be virtually indistinguishable in their kinetic con-stants for the inhibition of NE and Pr3: both werefast-acting inhibitors with...
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Tài liệu Báo cáo khoa học: FGF-2, IL-1b and TGF-b regulate fibroblast expression of S100A8 doc
... was > 90–97%.To determine whether de novo protein synthesis wasrequired for induction of S100A8 mRNA, confluent 3T3cells were preincubated with the protein synthesis inhibitor,cycloheximide ... organization in vitro. J Cell Biochem88, 848–854.9 Ito M & Kizawa K (2001) Expression of calcium-bind-ing S100 proteins A4 and A6 in regions of the epithelialsac associated with the onset of hair ... are members of the S100 family of Ca2+-binding proteins and are now accepted as markers of in ammation. They areexpressed by keratinocytes and in ammatory cells in human ⁄ murine woundsand...
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Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
... binding play no (or a very minor)role in the hydrolysis of oligomeric substrates. The SBS is located too far from the active site to in uence the binding and catalysis of these substrates in ... engineering of the SBS of XBS, the resultsobtained in the previous screening experiment directlyTable 1. The effect of genetic engineering of the secondary bind-ing site of the B. subtilis and A. niger ... discrepancy could be the use of a His-tagged protein in their study. The C-ter-minal location of this His-tag suggests a likely interfer-ence with the functionality of the SBS because threeimportant...
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Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx
... attack of the L-hOrn3a-amino group onto l-haOrn4. Takinginto account that the synthetases involved in the bio-synthesis of the DKP-inheriting toxins thaxtominand fumitremorgin also contain ... characterization of these compounds is growing and may result in the synthesis of specificinhibitors based on the structure of the pathogensiderophore [16].A promising approach for the isolation of secondarymetabolites, ... derivatization-basedelucidation of the overall stereochemistry. Further-more, the functional properties of erythrochelin actingas an iron-chelating compound were investigated. On the basis of the analysis of the S....
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Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf
... interaction with the PDZproteins: Mint-3, AMPA binding protein and gluta-mate receptor interacting protein (GRIP). It wouldtherefore be interesting to determine whether this motif is also involved in ... identified the GRASP55 bind-ing site in the ICD of MT1-MMP, as well as the GRASP55 domains involved in the interaction with MT1-MMP ICD. We also describe the GRASP55interaction with the furin ICD, ... dem-onstrating that the mutation of the whole LLY573motif is needed to abolish the interaction of MT1-MMP ICD with GRASP55. The importance of the LLY573motif in the MT1-MMP ICD observed in the...
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Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf
... C-terminal cysteine of the CXXCmotif is proline (Fig. S6). In WhiB2 the absence of proline and therefore of the kink might not allow the protein to attain an optimal geometry, even thoughthere is a CXXC ... mechanisms forresistance to intracellular killing, the scavenging of freeradicals and the reactivation of degenerated proteinsduring infection by a family of proteins named ‘thio-redoxins’ is ... is located at the N-terminus of a-2 helix of thioredoxin fold and is separated by akink from rest of the helix due to presence of a proline[25,48–50]. The kink provides proper positioning of the...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... mutant forms of MCPIP, eitherentirely lacking the PIN domain or with point mutations in this domain,were used. The results of experiments with actinomycin D indicate that lowerlevels of IL-1b ... on the type of MCPIP expressed by the vector introduced into these cells. In control cells(empty vector) and cells overexpressing a mutant form of MCPIP (without the PIN domain, or MCPIP with two ... spanning the entire coding sequence of the MCPIP gene or the b-actin gene (control). In comparison with the b-actin transcript, the level of MCPIP mRNA was very low. From the analyzed tis-sues,...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... 5333–5346 ª 2006 The Authors Journal compilation ª 2006 FEBS Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines Yuanying Ding1, Yong ... and protein are expressed in the eight human cancer cell lines examined, and HO-1 expression is detectable in five of the cell lines, including HeLa cervical cancer and HepG2 hepatoma. Down-regulation ... mRNA expression, expression of HO-1 protein was alsoinduced by the treatment with HO-2 siRNA in HepG2cells. These results indicate that the down-regulation of HO-2 expression is associated with induction of...
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Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc
... all the genesinvolved in the pathway in the same operon; the cell can then respond quickly to changes in the environ-ment by changing the expression of only a few genesand using the protein-synthesizing ... show that the data fit well with the indi-vidual determination of flux control coefficients byPFK, PK and LDH. The role of the las operon is dis-cussed on the basis of the distribution of flux controlfor ... by replacing the native laspromoter with synthetic promoters in a single cross-over event [11]. From this library consisting of 50strains with altered expression of the las operon, the enzyme...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ