Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt

... 4681 Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r Daniel E. Alete1, Mark E. Weeks2, Ara G. Hovanession3, Muhamed ... that muscle cell surface- associated nucleolin represents at least part of the muscle bind-ing site for axonal receptor protein tyrosine phosphatase-r and that nucle-olin may also be a necessary ... binding affinityare unrealistic at this stage. Nucleolin localization in muscle is analogous toPTPr ligand localizationTo determine whether the nucleolin identified by affin-ity chromatography is potentially...

Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx

... CTCTTTGATGACAGAAGTGCCT-3¢Syndecan-4 5¢-85 GAGTCGATTCGAGAGACTGA-3¢ 3655¢-450 AAAAATGTTGCTGCCCTG-3¢Glypican-1 5¢-566 GAATGACTCGGAGCGTACACTG-3¢ 4885¢-1054 CCTTTGAGCACATTTCGGCAA-3¢P450 aromatase ... 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ 2895¢-1821CAAGGGTAAATTCATTGGGCTTGG-3¢b-actin 5¢-2350 ACAGACTACCTCATGAAGAT-3¢ 6655¢-3222 AGCCATGCCAAATGTCTCAT-3¢Fig. 1. Dose-related eect of bFGF on FSH-stimulated ... interassay coef®cients of variation were lessthan 10%. The analysis o f the radioimmunoassay data wasperformed using the SECURIAprogram from the PackardInstrument Company (Meriden, CT, USA).DNA...

Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx

... 5¢-AGGACUUCUUGAAAGGCAACAUUAAAG-3¢, antisense, 3¢-UAUCCUGAAGAACUUUCCGUUGUAAUU-5¢; scrambled HO-2 siRNA: sense,5¢-UAUAAGAGUCAGUACACAUCAUGGAAG-3¢,anti-sense, 3¢-UAAUAUUCUCAGUCAUGUGUAGUACCU-5¢.Another ... cDNAwere: forward, 5¢-AAGCTTCATGTCAGCGGAAGTGGAAAC-3¢; reverse, 5¢-CTGCAGTCACATGTAGTACCAGGCCAA-3¢. The sequence underlined is an artiﬁcialHindIII site. A full-length HO-2 cDNA fragment was iso-lated ... The intracellular concentration of heme is maintained by the rate of its synthesis and degrada-tion [1]. Many enzymes and their regulators areresponsible for heme synthesis [1,2]. On the otherhand,...

Tài liệu Báo cáo khoa học: Cell-free translation systems for protein engineering docx

... such as T7 and SP6RNA polymerases. Translation requires factors for initiation, elonga-tion and termination, as well as components for aminoacylation oftRNA, such as amino acids, tRNA and ATP. ... YokogawaT, Okuni T, Nakayama H, Takio K, Yabuki T, KigawaT, Kodama K, Yokogawa T, Nishikawa K &Yokoyama S (2002) An unnatural base pair for incor-porating amino acid analogs into proteins. ... synthetases [38,39] and ribozymes [40] thatcan catalyze aminoacylation of tRNA with speciﬁcunnatural amino acids. In addition to amber codons,other target codons have been utilized for the samepurpose....

Tài liệu Báo cáo khoa học: Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay docx

... Srinivasan A, Viswanathan K, Raman R, Chandraseka-ran A, Raguram S, Tumpey TM, Sasisekharan V &Sasisekharan R (2008) Quantitative biochemical ratio-nale for differences in transmissibility ... to and agglutinate redblood cells (RBCs) is measured.In the case of inﬂuenza, for example, the hemagglu-tination assay takes advantage of the fact that hemag-glutinin (HA) on the surface ... characterization of the N-linked sialylat-ed glycan receptors expressed on the cell surface ofhuman bronchial epithelial cells (HBE), a natural target for infection by human-adapted inﬂuenza A...

Tài liệu Báo cáo khoa học: An anthrax lethal factor mutant that is defective at causing pyroptosis retains proapoptotic activity pdf

... Bergsbaken T & Cookson BT (2008)Anthrax lethal toxin and Salmonella elicit the common cell death pathway of caspase-1-dependent pyroptosisvia distinct mechanisms. Proc Natl Acad Sci USA 105,4312–4317.16 ... phosphorylationin a melanoma cell line, but in contrast to what wasobserved in RAW 264.7 cells, the mutant was able toefﬁciently kill these cells. These data are consistentwith the notion that induction ... Medicine and Pathobiology, University of Toronto, CanadaIntroductionBacillus anthracis lethal toxin (LeTx) is a binary toxinthat is released by the bacterium during an infection.It consists of a...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf

... L, Anderson SA & Massague´J(2004) Integration of Smad and forkhead pathways in the control of neuroepithelial and glioblastoma cell pro-liferation. Cell 117, 211–223.50 Barreto G, Scha¨fer ... not easily available for RNA polymerase II. Only a small portion of P-TEFbkinase is already associated with BRD4, an activatorof P-TEFb kinase which is able to directly bind to his-tone proteins.Recently, ... functional analysis of the above-mentionedfusion partner proteins – AF4 (family members), AF9,ENL and AF10 – has shed light on the activation cycleof P-TEFb kinase. All assemble in a high-molecularmass...

Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

... 5¢-CCTTCA ATG ACA ACG ACA CCG-3¢ (forward) and 5¢-CCATGC GAC TTG TCA GGC T-3 ¢ (reverse); glyceraldehyde-3-phosphate dehydrogenase, 5¢-GAC ATC AAG AAGGTG GTG AAG C-3¢ (forward) and 5¢-GTC CAC CACCCT ... was performed at least three times, and the data were expressed as mean ± standard error of the mean, or representative data were shown. The statisticalanalysis was performed using a two-tailed ... 3780–3788 ª 2010 The Authors Journal compilation ª 2010 FEBSplays an important role in the activation of endothelialcells and macrophages, as well as the differentiationand proliferation of VSMCs....

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... suggests that calpain 7 also functions as a protease rather than as a structural protein in mamma-lian cells, and that MIT domains are involved in cal-pain 7 activation. This notion leads us to ... amino acids containing four tandem MP repeats,obtained from Kazusa DNA Research Institute (Chiba,Japan), using a pair of primers (forward, 5¢-CTAGAATTCAACAGCACAGCATGCTGG-3¢; reverse, 5¢-AGAGAATTCTGCCTGGTTTAAGAGACC-3¢; ... by WB analysis with mAb against GFP orpolyclonal antibody (pAb) against calpain 7 (raisedagainst recombinant MIT domains [28]). In the case ofWB analysis with mAb against GFP, 45 K and 30...

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... wereused as primary antisera (Table 3). Secondary antibodies(anti-rat IgG, anti-guinea pig IgG and anti-rabbit IgG; allraised in goat and conjugated to alkaline-phosphatase;Sigma, Saint Louis, ... 91–95.53 Morishita F, Nakanishi Y, Kaku S, Furukawa Y, OhtaS, Hirata T, Ohtani M, Fujisawa Y, Muneoka Y &Matsushima O (1997) A novel D-amino-acid-containingpeptide isolated from Aplysia heart. ... 4036–4039.Table 3. Primary antisera used in the present study, antigens for antisera production, animals immunized for primary antisera production,and secondary antisera used in immunohistochemistry...

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