Tài liệu Báo cáo khoa học: Directed evolution of trimethoprim resistance inEscherichia coli doc

... indicated. Directed evolution in E. coli M. Watson et al.2666 FEBS Journal 274 (2007) 2661–2671 ª 2007 The Authors Journal compilation ª 2007 FEBS Directed evolution of trimethoprim resistance in ... [5].Because of its central role in one-carbon metabo-lism, dihydrofolate reductase (DHFR) has long been aKeywordsantibiotic resistance; dihydrofolatereductase; directed evolution; trimethoprim CorrespondenceM. ... in directed evolution also enable us to pro-duce multiple mutant forms of the enzyme, all of whichhave potentially used different methods to overcomethe obstacles present in the directed evolution...

Tài liệu Báo cáo khoa học: Molecular evolution of shark and other vertebrate DNases I pptx

... 3¢-UTR of vertebrate DNase I cDNA lengthened abouttwofold during the evolutionary stage between amphibiansand reptiles.Structural features of shark DNases IThe amino acid sequences of the ... rlysates of cells that had been transfected with expressionvectors containing the entire coding regions of the wild-typeshark DNases I, substitution of the signal sequence regions of each of the ... hydrophobicity proﬁles of the signal sequence regions of human, eel, frog, T. scyllia and H. japonicus DNases I.However, prediction of the secondary structure of thecorresponding part of the enzyme...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... several cases, mod-erate resistance is imparted by mutations of the targetenzyme (target-site mechanism of resistance) [60], butthere is, as yet, no documented case of a plant specieshaving ... molecular mode of action of glypho-sate and the molecular basis for glyphosate resistance. Structure and function of EPSPSEPSPS catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate ... herbicide resistance. A number of promisingenzymes were identiﬁed by selective evolution, site- directed mutagenesis, and microbial screens [21,22].L. Pollegioni et al. Mechanisms of glyphosate resistance FEBS...

Tài liệu Báo cáo khoa học: "The Nature of Affixing in Written English" docx

... problem of unscrambling English affixes. The role of affixes is crucial in the study of word-breaking practice. In the automatic determination of the parts of speech (a central feature of automatic ... determination of parts -of- speech assignments E. The determination of the number of phonetic syl- lables corresponding to a written English word In the first case, we have taken a random sample of 100 ... obvious algorithms needed for most of the words and put this together with a list of irregular forms for a working procedure, except for the presence of a number of verbs where it is necessary...

Tài liệu Báo cáo khoa học: "Structural Definition of Affixes from Multisyllable Words" docx

... classification of the weak suffixes by degree of weakness as a means of approaching the suffix-in- context problem. Table 2, which reviews the prefixes and suffixes de- fined by Resnikoff and Dolby, ... English," an algorithm for the structural definition of affixes was developed and applied to data consisting of all the words of the form CVCVC in the Shorter Oxford Dictionary. Fourteen ... two-vowel-string words. This paper summarizes the results of applying a modified form of the operational definition to data consisting of all the four-, five-, six-, and seven-vowel-string words...

Tài liệu Báo cáo khoa học: "A GENERALIZATION OF THE OFFLINE PARSABLE GRAMMARS" doc

... f'mite set of symbols, so there is only a finite number of strings of given length L, and it has a finite number of rules, so there is only a finite number of possible parse trees of given depth ... representation for the set of pairs of ground terms [A,B] such that (A > B) is a rule in the ground grammar of G'. Clearly this representation is just the set of pairs of terms [C,D] such ... set of ground instances of lists in D n is the set of chain derivations of length n in the ground grammar for G'. Once again, the proof is from basic properties of unification. The lists...

Tài liệu Báo cáo khoa học: Identiﬁcation of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis docx

... effect of chloride ions on enzyme activitywas investigated (Fig. 4). The hydrolysis of Mca-Fig. 3. Role of His505 and His345 in catalysis. Schematic of theproposed reaction intermediate of ACE2, ... study has highlighted the importance of Arg273in binding of the C-terminus of peptide substrates byACE2. The complete loss of activity observed as aresult of replacing this single residue could ... nitrogen) of both residues arewithin hydrogen-bonding distance of the carbonyloxygen of the amide group of residue P1¢ (tACE)(Fig. 1B) or equivalent terminal carboxylate oxygen(ACE2) (Fig. 1A) of...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... revealed tau bands of  64 kDa in the GST–Fyn-SH3-bound fraction from cells transfected with each of the tau constructs. Densitometric analysis of Fyn-SH3-bound tau as a proportion of that in correspondingcell ... this band was notapparent on western blots of lysates of Y394F tau(Fig. 2A). The absence of this larger species could indi-cate that phosphorylation of tau by Fyn is reducedand therefore, in ... tau revealeddecreased electrophoretic mobility of tau, with theappearance of an  68-kDa tau species in wild-type andall of the mutant forms of tau except for YallF andY394F tau (Fig. 3)....

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... surface of the molecule and is ratherfar away from the trinuclear site. Therefore, theC-terminal sequence of RlL might be more of an evolu-tionary relic than a functional feature of the enzyme.The ... molecules A and B(and C and D) of the asymmetric unit form a weakdimer (Fig . 6). On the basis of calculations performedFig. 6. (A) Cartoon representation of dimers of TaLcc1 (blue) and rMaL (green). ... buffer at pH 6.0 (25 °C). Structural formulas of the substrates are presented. Redox potentials (E°)ofT1coppers of the laccases and redox potentials of the substrates at pH 4.5 and pH 6.0 are provided,...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... reservoir consisted of 500 lL of 0.7 m sodium citrate,10 mm dithiothreitol and 70 mm Hepes at pH 7.4. Dropsconsisted of 0.75 l L of importin-a (70-529) at 18 mgÆmL)1,0.75 lL of CLIC4 NLS peptide ... Indicative of thetightness of the ﬁt, there are a large number (30) of atom-to-atom van der Waals’ contacts between theTyr205 side chain and importin-a (Table 2). The num-bers of contacts ... through asuite of programs in the ccp4 crystallography package [36].Scaling of intensities and inspection of data quality wereperformed in scala. In particular, the resolution cut-offwas determined...

Xem thêm

Từ khóa: tài liệu báo cáo khoa học bản chất của khủng hoảng kinh tế thế giới pdf tài liệu báo cáo nghiên cứu khoa học tài liệu về báo cáo khoa học báo cáo khoa học tài chính công báo cáo khoa học số loài quý hiếm tại vườn quốc gia ba bể tai lieu bao cao thuc tap khoa co khi Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018 Báo cáo quy trình mua hàng CT CP Công Nghệ NPV Nghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoại Một số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTP Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Phát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninh Nghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000 Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tin Thơ nôm tứ tuyệt trào phúng hồ xuân hương Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIX Chuong 2 nhận dạng rui ro Tổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vật chuong 1 tong quan quan tri rui ro Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật Chiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015