Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx

... mam-mals cannot synthesize folates because of the lack of three enzymes in the middle of the folate pathway, and they therefore obtain folates from the diet. DHNA is the ﬁrst of the three enzymes that ... obtained bysubtracting the control titration data in the absence of the enzymes from the titration data in the presence of the enzymes. The results of the equilibrium binding studies showed that, ... aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes Yi Wang, Yue Li, Yan Wu and Honggao YanDepartment of Biochemistry and Molecular Biology,...

Tài liệu Báo cáo khoa học: Mechanism of protection of peroxidase activity by oscillatory dynamics docx

... termination of the degradation of the enzyme [12], because the inhibition of the reaction alsoblocks the formation of free radicals [28]. Thus, the inactivation of the enzyme can be ascribed to the ... role in the dynamics of the peroxidase–oxidase reaction [13], and therefore theydo not appear in most detailed models of the reaction. The pronounced dependence of the stability of the enzyme ... for inactiva-tion of the enzyme may come from measurement of the effect of the concentration of the aromatic cofactorresponsible for the onset of oscillations. Here we use the fact that melatonin...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... modiﬁcations of the MT1-MMP ICD have also been reported with the palmitoy-lation of the cysteine 574 (C574) residue [30] and the phosphorylation of the tyrosine 573 (Y573) [31] and threonine ... Interestingly,mutation of the LLY573motif led to the detection of pro-MT1-MMP in the immunoprecipitate, sugges-ting that the disruption of the interaction betweenMT1-MMP and GRASP55 could affect the activation of ... furin-dependent mechanism, with the bulk of activation being GRASP55-independent. The interaction observed between GRASP55 and PC5/6B or PC7 together with the potential role of theseproprotein-convertases...

Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt

... A. nicotinovorans is linkedto the presence of the megaplasmid, pAO1 [20]. The recentelucidation of the DNA sequence of pAO1 revealed the modular organization of the enzyme genes involved innicotine ... mutagenesis of the MABOgene The amino acid substitutions in the MABO protein weremade with the aid of the Quick Change site-directedmutagenesis kit (Stratagene), according to the instructions of the ... reduction o f the ﬂavincofactor.Site-directed mutagenesis of MABOAn amino acid alignment of the N-terminal sequence of pAO1 MABO, with the sequence of related enzymes, isshown in Fig. 5A. The alignment...

Tài liệu Báo cáo khoa học: Mammalian mitotic centromere-associated kinesin (MCAK) A new molecular target of sulfoquinovosylacylglycerols novel antitumor and immunosuppressive agents pptx

... functions such as vesicle trans-port, chromosome segregation, and organization of the mitotic spindle. Therefore, one of the other targets of the SQAGs is probably a MCAK. We show here thatSQAGs suppress ... binding. The tightness of binding maydetermine the degree of inhibition.SQAGs inhibit the depolymerization activity of MCAK in vivoTo elucidate the MT-depolymerizing effects of SQAGsin vivo, the ... MCAK184 alone, and with MCAK184 and AMPPNP. Depoly-merized microtubules were visualized in the lane of the supernatant(S), and polymerized in the lane of pellet (P). (B) SQAGs inhibited the depolymerization...

Tài liệu Báo cáo khoa học: "Inducing Gazetteers for Named Entity Recognition by Large-scale Clustering of Dependency Relations" ppt

... question is whether thesegazetteers improve the accuracy further when theyare used together. The accuracies of models thatuse the Wikipedia gazetteer and one of the clustergazetteers at the same ... this further.4.5 Effect of Combining the Cluster and the Wikipedia GazetteersWe have observed that using the cluster gazetteer and the Wikipedia one improves the accuracy of Japanese NER. The next ... 16% of the matches werelost as a result of using our left-most longest heuris-tics for the case of the c500k-2k gazetteer.A comparison of the effect of these gazetteers isshown in Table 5. The...

Tài liệu Báo cáo khoa học: "ModelTalker Voice Recorder – An Interface System for Recording a Corpus of Speech for Synthesis" ppt

... recorded, the user rece-ives feedback on the overall quality of the utter-ance. Specifically, the user receives feedback on the pitch, the overall amplitude, and the pronuncia-tion of the recording. ... smoother, more natural sounding synthesis, but requires many hours of recording, often by a trained professional. The recording process is usually supervised, and the recordings are often hand-polished. ... record a corpus of speech. The system provides the user with feedback on the quality of each utterance they record in terms of pronunciation accuracy, relative uniformity of pitch, and relative...

Tài liệu Báo cáo khoa học: "Word Alignment for Languages with Scarce Resources Using Bilingual Corpora of Other Language Pairs" pptx

... that the po-sition probability is independent of the position of the Chinese word and the length of the Chinese sentence. And we assume that the position probability is in-dependent of the ... (2)ml, are the lengths of the source sentence and the target sentence respectively. j is the position index of the target word. ja is the position of the source word aligned to the jth ... words represents the relative position of the head word of the i)(11 −−ijd ⊙th cept and the center of the (i-1)th cept. Let 1−−=Δijj ⊙ , then is independent of the absolute position....

Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc

... 30821065 and 30870559 from the China National Nature SciencesFoundation, 2006CB910703 from the Ministry of Sciences and Technology of China, and 07dz05907from the Committee of Sciences and Technology ... 15-deoxy-D12,14-prosta-glandin J2 (PGJ2), and reversed by GW9662, aPPARc antagonist. Further analysis suggests thatTZDs induce the downregulation of Akt-1 and lead to the activation of caspase-3-dependent ... decreased the protein level of prosurvival Bcl-2, but not of prodeath Bax(Fig. 4A). Consequently, the Bax ⁄ Bcl-2 ratio wasincreased, promoting apoptosis.In the Bcl-2 family, the disequilibrium of...

Tài liệu Báo cáo khoa học: It is all about resolution Meeting report based upon presentations at the 10th International Global BioMillennium 2006 symposium on molecular cell biology (Tbilisi, Georgia) docx

... In the absence of interferon, STAT2 perma-nently and rapidly shuttles between the cytoplasm and the nucleus. A region in the C-terminus of STAT2controls its specific export in the absence of ... synchronization of cell cycle events, with one goal being the develop-ment of specific microRNA and protein signatures of particular tumor cell types, and another being to fol-low the dynamics of neurite ... University of Jerusalem, Israel2 Department of Virology, The Hebrew University of Jerusalem, IsraelIntroductionImproved resolution in time and space is the hallmark of studies in diverse subfields of...

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